Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Antidiabetic Activities Analysis by Oral Glucose Tolerance Test in Rats

  • Received : 2010.11.29
  • Accepted : 2010.12.03
  • Published : 2010.12.31
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Abstract

In this study, we conducted an oral glucose tolerance test (OGTT) so as to compare antidiabetic activities of general potatoes, purple-flesh potatoes, and potato pigments in rats at various concentration levels. After allowing the rats to abstain from food for 12 hours, 10%/20% general potato, purple-flesh potato, and potato extract was orally administered to rats at 100 and 500 mg/kg concentrations. The blood glucose level was measured after an hour. Then, immediately, 1.5 g/kg of sucrose was administered through the abdominal cavity and the blood glucose measured after 30, 60, 120, and 180 minutes. 20% purple-flesh potato group and 10% general potato group, both 100 and 500 mg/kg, showed a significant concentration-dependent decrease in blood glucose levels after 30 minutes. The 100 mg/kg potato pigment group also showed a statistically significant decrease after 30 minutes. In conclusion, administration of 10% general potato, 20% purple-flesh potato, and potato pigment can reduce blood glucose level in an OGTT using rats.

Keywords

References

  1. Acquaviva R, Russo A, Galvano F (2003): Cyanidin and cyaniding 3-O-beta-D-glucoside as DNA cleavage protectors and antioxidants. Cell Biol Toxicol 19: 243-252. https://doi.org/10.1023/B:CBTO.0000003974.27349.4e
  2. Ahn KJ (2010): Westernization of Korean diabetes. Korean Clinical Diabetes 91-94.
  3. Baynes JW (1991): Role of oxidative stress in development of complications in diabetes. Diabetes 40(4): 405-412. https://doi.org/10.2337/diabetes.40.4.405
  4. Baynes JW, Thorpe SR (1996): The role of oxidative stress in diabetic complications. Current Opinionin Endocrine 3:277-284. https://doi.org/10.1097/00060793-199608000-00001
  5. Brown CR (2005): Antioxidants in potato. Am J Potato Res 82:163-172. https://doi.org/10.1007/BF02853654
  6. Hyon SH, Kim DH (2001): Long-term preservation of rat pancreatic islets under physiological conditions. J Biotechnol 85:241-246. https://doi.org/10.1016/S0168-1656(00)00336-9
  7. Kahn CR (1985): The molecular mechanism of insulin action. Annu Rev Med 36:429-451. https://doi.org/10.1146/annurev.me.36.020185.002241
  8. Lazze M, Pizzala R, Savio M, Stivala L, Prosperi E, Bianchi L (2003): Anthocyanins protect against DNA damage induced by tert-butyl-hydroperoxide in rat smooth muscle and hepatoma cells. Mutat Res 53:103-115.
  9. Lefevre M, Howard L, Most M, Ju Z, Delany J (2004): Microarray analysis of the effects of grape anthocyanins on hepatic gene expression in mice. FASEB J 18: 851.
  10. Matsui T, Ebuchi S, Kobayashi M, Fukui K, Sugita K, Terahara N, Matsumoto K (2002): Anti-hyperglycemic effect of diacylated anthocyanin derived from Ipomoea batatas cultivar Ayamurasaki can be achieved through the $\alpha$-glucosidase inhibitory action. J Agric Food Chem 50:7244-7248. https://doi.org/10.1021/jf025913m
  11. Park YE, Cho JH, Cho HM, Yi JY, Seo HW, Chung MG (2009): A new potato cultivar "Hongyoung", with red skin and flesh color, and high concentrations of anthocyanins. Korean J Breed Sci 41:502-506.
  12. Reeves PG, Nielsen FH, Fahey Jr GC (1993): AIN-93 purified diets for laboratory rodents: final report of the American institute of nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nut 123:1939-1951.
  13. Wolff SP (1993): Diabetes mellitus and free radicals. Br Med Bull 49:642-652.