Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Effects of Plant Extract containing Creams on UVB Radiation-induced Inflammatory Responses in Mice

쥐에서 자외선 B가 유도한 염증 반응에 대한 식물 추출물 함유 크림의 영향

  • An, Sang-Mi (Department of Molecular Medicine and Cell and Matrix Research Institute, Kyungpook National University School of Medicine) ;
  • Lee, Seung-Jin (Department of Molecular Medicine and Cell and Matrix Research Institute, Kyungpook National University School of Medicine) ;
  • Park, Kwon-Moo (Department of Anatomy, Kyungpook National University School of Medicine) ;
  • Koh, Jae-Sook (Dermapro Skin Research Center, Dermapro Ltd.) ;
  • Boo, Yong-Chool (Department of Molecular Medicine and Cell and Matrix Research Institute, Kyungpook National University School of Medicine)
  • 안상미 (경북대학교 의학전문대학원 분자의학교실 세포기질연구소) ;
  • 이승진 (경북대학교 의학전문대학원 분자의학교실 세포기질연구소) ;
  • 박권무 (경북대학교 의학전문대학원 해부학교실) ;
  • 고재숙 ((주)더마프로 피부과학연구소) ;
  • 부용출 (경북대학교 의학전문대학원 분자의학교실 세포기질연구소)
  • Received : 2010.10.12
  • Accepted : 2010.12.02
  • Published : 2010.12.30
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Abstract

Ultraviolet radiation (UV) is a major cause of photodamages to human skin and the immediate responses of the skin to UV include the erythema and edema. In an attempt to find effective UV-protecting agents to be used in cosmetics, a number of plant extracts were screened in the cell-based assays. Among the total of 38 plant extracts tested, 3 plant extracts derived from Sasa quelpaertensis, Althaea rosea, and Dryopteris crassirhizoma attenuated the UVB-induced cytotoxicity as well as melanin synthesis in cultured human epidermal melanocytes. The anti-inflammatory effects of these plant extracts were further examined in animal models. A control or test cream containing 1% of a plant extract was topically applied to ears of a C57BL/6 mouse or the dorsal skin of a SKH-1 hafirless mouse before and after the exposure to UVB. The change in ear thickness or dorsal skin redness due to UVB exposure was determined to monitor edema and erythema, respectively. All three test creams exhibited anti-inflammatory effects in both experiments. The creams containing Sasa quelpaertensis, Althaea rosea or Dryopteris crassirhizoma extract alleviated the UVB-induced edema response on day 4 by 53.8 %, 56.4 % and 31.1 %, respectively. They also inhibited the erythema formation on day 2 by 45.7 %, 34.1 % and 20.5 %, respectively. This study suggests that the selected plant extracts formulated in cosmetics may attenuate skin inflammation caused by overexposure to UV.

자외선(UV)은 인체 피부 손상의 주된 요인이고 UV에 대한 피부의 초기 반응들은 홍반과 부종을 포함한다. 화장품에 사용할 효과적인 자외선 방어제를 발굴하기 위하여 세포수준의 효능평가법으로 다양한 식물 추출물들을 검색하였다. 시험된 총 38종의 식물 추출물들 중 제주조릿대(Sasa quelpaertensis), 접시꽃(Althaea rosea), 관중(Dryopteris crassirhizoma)에서 유래한 3종의 식물 추출물이 배양된 인체 표피 멜라닌세포에서 멜라닌 생성뿐만 아니라 UVB의 세포독성을 경감시켰다. 이 식물 추출물들의 항염증 작용은 동물 실험에서도 평가하였다. 대조 크림 혹은 식물 추출물을 1 % 함유한 시험 크림을 C57BL/6 쥐의 귀에 혹은 SKH?1 무모쥐의 등에 UVB 조사 전과 후에 도포하였다. UVB 노출에 의한 영향으로서 부종 생성은 귀의 두께 변화를, 홍반 생성은 등 피부의 붉기의 변화를 측정하여 추정하였다. 3종류의 시험 크림 모두 두 실험 모두에서 항염증효과를 보였다. 제주조릿대, 접시꽃, 또는 관중 추출물을 함유한 크림은 자외선 조사 4일째 부종 반응을 각각 53.8 %, 56.4 %, 31.1 % 경감시켰다. 또한 이 크림들은 자외선 조사 2일째 홍반 생성을 각각 45.7 %, 34.1 %, 20.5 % 억제시켰다. 본 연구는 화장품에 함유된 특정 식물 추출물들이 자외선 과다노출로 야기되는 피부 염증을 완화시켜 줄 수 있음을 시사하였다.

Keywords

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