Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
권호 표지

Photodynamic Therapy for Methicillin-resistant Staphylococcus aureus with High-level Mupirocin Resistance using 630 nm Light-emitting Diode

  • Kwon, Pil-Seung (Department of Clinical Laboratory Science, Wonkwang Health Science University) ;
  • Kim, Jin-Kyung (Department of Microbiology & Vestibulocochlear Research Center, Wonkwang University College of Medicine)
  • Received : 2010.11.05
  • Accepted : 2010.12.30
  • Published : 2010.12.31
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Abstract

This study aims to evaluate the effect of Photodynamic Therapy (PDT) against methicillin-resistant Staphylococcus aureus with high-level mupirocin resistance (Hi-Mup MRSA). To examine the antimicrobial effect of photogem-mediated PDT against Hi-Mup MRSA, CFU quantifications, bacteria cell viability tests, and disk diffusion antimicrobial susceptibility tests were evaluated. In addition, one of PDT mechanisms was investigated by accumulating photogem ($10\;{\mu}g/ml$) in Hi-Mup MRSA. Photogem-mediated PDT properly inhibited the colony formation of Hi-Mup MRSA. Viable bacteria decreased greatly after a PDT application with photogem $10\;{\mu}g/ml$ at energy density $15\;J/cm^2$. The diameter of the inhibition zone around susceptible disks increased after PDT. In addition, we confirmed the accumulation of photogem in bacteria through fluorescent images. These results demonstrated that excellent photosensitization of Hi-Mup MRSA can be achieved using photogem with 630 nm LED irradiation. Thus, PDT may make survival Hi-Mup MRSA inactive.

Keywords

References

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