Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Caffeic Acid Phenethyl Ester Inhibits Cell Proliferation and Induces Apoptosis in Human Ovarian Cancer Cells

  • Park, Hyung-Joo (Asan Institute for Life Sciences, Asan Medical Center) ;
  • Yang, Seung-Joo (Asan Institute for Life Sciences, Asan Medical Center) ;
  • Mo, Jin-Young (Asan Institute for Life Sciences, Asan Medical Center) ;
  • Ryu, Geun-Chang (Department of Optometry and Optic Science, Dongshin University) ;
  • Lee, Kyung-Jin (Asan Institute for Life Sciences, Asan Medical Center)
  • Received : 2010.10.25
  • Accepted : 2010.11.10
  • Published : 2010.12.01
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Abstract

The phenethyl ester of caffeic acid (CAPE), an active component of honeybee propolis extract, is shown to inhibit cancer growth previously. However, studies on human ovarian cancer are largely obscure. This study evaluated the effects of CAPE as a potential anti-proliferative and pro-apoptotic agent in the human ovarian cancer line, OVCAR-3. CAPE treated OVCAR-3 cells showed inhibition of cell viability and proliferation in a dose-dependent manner by WST-1 assay, LDH assay and bromodeoxyuridine (BrdU) incorporation assay. Furthermore, CAPE-mediated OVCAR-3 cell growth inhibition was associated with apoptotic changes as evident by cell cycle arrest and accumulation of cells in the apoptotic phase and DNA fragmentation. Taken together, CAPE inhibits cell proliferation via DNA synthesis reduction and induces apoptotic cell death via DNA damage, thus elucidating a novel, plausible mechanism of CAPE anti-tumorigenic property in OVCAR-3 cells.

Keywords

References

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