Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Performance Comparison of 3 Different Isolation Media of Cronobacter sakazakii

Cronobacter sakazakii 분리배지의 성능 비교

  • 김현정 (한국식품연구원 안전성연구단) ;
  • 구민선 (한국식품연구원 안전성연구단) ;
  • 오세욱 (국민대학교 식품영양학과)
  • Received : 2010.01.25
  • Accepted : 2010.03.15
  • Published : 2010.05.31
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Abstract

Three different isolation media for Cronobacter sakazakii have been recommended by Korea Food and Drug Administration from 2007. Performance comparison test was carried out between recommended Cronobacter sakazakii isolation medium. Chromogenic Enterobacter sakazakii agar (CESA) and Enterobacter sakazakii agar (ESA) produce more distinctive colonies having characteristic colors and appearance than Violet red bile glucose agar (VRBGA). The sensitivity and specificity of 3 different isolation media was checked. All 3 tested media showed 100% sensitivity when tested with 30 different Cronobacter sakazakii. The CESA and ESA showed 100% specificity when tested with Enterobacteriaceae except Cronobacter sakazakii, However, VRBGA did not show any specificity, showing inadequate selectivity compared to applicable Cronobacter sakazakii isolation medium. Artificially inoculated Cronobacter sakazakii to milk powder was easily recovered with 3 different isolation media and they all showed almost the same recovery activity.

식품공전에 Cronobacter sakazakii 분리배지로 등재되어 있는 3종의 분리배지에 대한 평가실험을 실시하였다. Chromogenic Enterobacter sakazakii agar, Enterobacter sakazakii agar가 VRBG agar에 비하여 뚜렷한 색택과 모양의 집락을 생성하였다. 3종의 분리배지 모두 30종의 Cronobacter sakazakii에 대한 sensitivity가 100%로 측정되었지만 Cronobacter sakazakii 이외의 Enterobacteriaceae 균주를 이용한 specificity 실험에서는 Chromogenic Enterobacter sakazakii agar, Enterobacter sakazakii agar가 100%로 측정되었지만 VRBG agar는 0%로 측정되었다. 인위적으로 접종한 식품에서의 회수율은 실험에 공시된 3종 배지에서 커다란 차이가 없었다.

Keywords

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