The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
권호 표지

Anti-Inflammatory Effects of Glycyrrhiza glabra Linne Extract in a Dextran Sulfate Sodium-Induced Colitis Mouse Model

감초 추출물의 Dextran Sulfate Sodium 유도 마우스 궤양성 대장염 억제 효과

  • 이경호 (코오롱 생명과학연구소) ;
  • 이기형 (공주대학교 산업과학대학)
  • Received : 2010.07.19
  • Accepted : 2010.10.01
  • Published : 2010.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

The aim of this study was to evaluate the anti-inlfammatory effects of Glycyrrhiza glabra Linne extract on ulcerative colitis induced by 3% dextran sulfate sodium in mice. The experimental animals were divided into six groups: control(normal), DSS-induced colitis(control), 1 mg/kg, 10 mg/kg, and 100 mg/kg of Glycyrrhiza glabra Linne extract, and 150 mg/kg 5-aminosalicylic acid(5-ASA)(positive control). We evaluated the pathological disease activity index(DAI), change in weight, colon mucosa damage and myeloperoxidase(MPO) in colon mucosa. Treatment with 10 mg/kg and 100 mg/kg of Glycyrrhiza glabra Linne extract led to significant loss of body weight, the decrease of MPO activity and clinical symptoms such as DAI and histological change. In particular, 100 mg/kg Glycyrrhiza glabra Linne extract led to markedly greater improvement than 150 mg/kg 5-ASA treatment. These results suggest that Glycyrrhiza glabra mediated anti-inflammatory action on colorectal sites may be a useful therapeutic approach to ulcerative colitis.

Keywords

References

  1. Adachi M, Kurotani R, Morimura K, Shah Y, Sanford M, Madison BB, Gumucio DL, Marin HE, Peters JM, Young HA, Gonzalez FJ. 2006. Peroxisome proliferator activated receptor gamma in colonic epithelial cells protects against experimental inflammatory bowel disease. Gut 55:104-1113
  2. Agarwal MK, Iqbal M, Athar M. 2005. Inhibitory effect of 18 $\beta-glycyrrhetinic$- glycyrrhetinic acid on 12-O-tetradecanoyl phorbol-13-acetateinduced cutaneous oxidative stress and tumor promotion in mice. Redox Rep 10:151-157 https://doi.org/10.1179/135100005X57346
  3. Ahn BO, Ko KH, Oh TY, Cho H, Kim WB, Lee KJ, Cho SW, Hahm KB. 2001. Efficacy of use of colonoscopy in dextran sulfate sodium induced ulcerative colitis in rats: The evaluation of the effects of antioxidant by colonoscopy. Int J Colorectal Dis 16:174-181 https://doi.org/10.1007/s003840000282
  4. Buchman AL. 2001. Side effects of corticosteroid therapy. J Clin Gastroenterol 33:289-294 https://doi.org/10.1097/00004836-200110000-00006
  5. Chang DK, Kim YH, Byeon JS, Yang SK, Chung YW, Han DS, Kim SG, Kim TI, Kim WH, Jeen YT, Eun CS, Choi H, Choi KY, Song IS. 2005. The current status of ulcerative colitisassociated colorectal cancer in Korea: A KASID study. Korean J Gastroenterol 46:276-282
  6. Chen WX, Chen JL, Da W, Chen NW, Zhu JS. 2002. Study on P-selectin and ICAM-1 of mouse with ulcerative colitis. World J Gastroenterol 10:722-724
  7. Cuzzocrea S, Mazzon E, Costantino G, Serraino I, Dugo L, Calabrò G, Cucinotta G, De Sarro A, Caputi AP. 2000. Beneficial effects of N-acetylcysteine on ischemic brain injury. Br J Pharmacol 130:1219-1226 https://doi.org/10.1038/sj.bjp.0703421
  8. Dieleman LA, Palmen MJ, Akol H, Bloemena E, Pena AS, Meuwissen SG, Van Rees EP. 1998. Chronic experimental colitis induced by dextran sulphate sodium(DSS) is characterized by Th1 and Th2 cytokines. Clin Exp Immunol 114: 385-391 https://doi.org/10.1046/j.1365-2249.1998.00728.x
  9. Dong WG, Liu SP, Wu DF, Luo HS, Yu JP. 2003. Ameliorative effects of sodium ferulate on experimental colitis and their mechanism in rats. World J Gastroenterol 9:2533-2538 https://doi.org/10.3748/wjg.v9.i11.2533
  10. Fan H, Qiu MY, Mei JJ, Shen GX, Liu SL, Chen R. 2005. Effects of four regulating-intestine prescriptions on pathology and ultrastructure of colon tissue in rats with ulcerative colitis. World J Gastroenterol 11:4800-4806 https://doi.org/10.3748/wjg.v11.i31.4800
  11. Frank K, Helmut M, Marianne A, Volker G, Tina B, Jürgen S, Josef R. 2001. Clinical and histopathological features of dextran sulfate sodium induced acute and chronic colitis associated with dysplasia in rats. Int J Colorectal Dis 16:238-246 https://doi.org/10.1007/s003840100311
  12. Hanauer SB, Sperrow M. 2004. Therapy of ulcerative colitis. Curr Opin Gastroenterol 20:345-350 https://doi.org/10.1097/00001574-200407000-00008
  13. Hanauer SB. 2005. Infliximab or cyclosporine for severe ulcerative colitis. Gastroenterology 129:1358-1359
  14. Hisamatsu T, Hibi T. 2005. Immunosuppressants for therapy of patients with ulcerative colitis. Nippon Rinsho 63:831-835
  15. Ishioka T, Kuwabara N, Oohashi Y, Wakabayashi K. 1987. Induction of colorectal tumors in rats by sulfated polysaccharides. Crit Rev Toxicol 17:215-244 https://doi.org/10.3109/10408448709071209
  16. Jeong HG, You HJ, Park SJ, Moon AR, Chung YC, Kang SK, Chun HK. 2002. Hepatoprotective effects of 18 $\beta-glycyrrhetinic$ acid on carbon tetrachloride-induced liver injury: Inhibition of cytochrome P450 2E1 expression. Pharmacol Res 46: 221-227 https://doi.org/10.1016/S1043-6618(02)00121-4
  17. Juskiewicz J, Zdunczyk Z. 2004. Effects of cellulose, carboxymethylcellulose and inulin fed to rats as single supplements or in combinations on their cecal parameters. Comp Biochem Physiol A Mol Integr Physio 139:513-519 https://doi.org/10.1016/j.cbpb.2004.10.015
  18. Kim WH, Cho JH, Kim TI. 2003. Rational dosing of azathioprine and 6-mercaptopurine in inflammatory bowel diseases. Korean J Gastroenterol 41:423-437
  19. Klotz U. 2005. Colonic targeting of aminosalicylates for the treatment of ulcerative colitis. Dig Liver Dis 37:381-383 https://doi.org/10.1016/j.dld.2004.12.011
  20. Lakatos L, Pandur T, David G, Balogh Z, Kuronya P, Tollas A, Lakatos PL. 2003. Association of extraintestinal manifestations of inflammatory bowel disease in a province of western Hungary with disease phenotype: Results of a 25-year followup study. World J Gastroenterol 9:2300-2307 https://doi.org/10.3748/wjg.v9.i10.2300
  21. Matsui S, Matsumoto H, Sonoda Y, Ando K, Aizu-Yokota E, Sato T, Kasahara T. 2004. Glycyrrhizin and related compounds downregulate production of inflammatory chemokines IL-8 and eotaxin 1 in a human lung fibroblast cell line. Int Immunopharmacol 15:1633-1644
  22. Podolsky DK. 2002. Inflammatory bowel disease. N Eng J Med 347:417-429 https://doi.org/10.1056/NEJMra020831
  23. Porter SN, Howaeth GS, Butler RN. 1998. An orally administered growth factor extract derived from bovine whey suppresses breath ethane in colitic rats. Scand J Gastroenterol 33: 967-974 https://doi.org/10.1080/003655298750027001
  24. Shibata S. 2000. A drug over the millennia: pharmacognosy, chemistry, and pharmacology of licorice. Yakugaku Zasshi 120:849-862 https://doi.org/10.1248/yakushi1947.120.10_849