In vitro Inhibitory Effect of Alcohol Hand Rubs Against Staphylococcus aureus

물 없이 사용하는 알코올 손소독제의 시험관 내 Staphylococcus aureus 증식억제 효과

  • Moon, Cheol (Department of Clinical Laboratory Science, Semyung University) ;
  • Kim, Kyung-Mi (Department of Nursing, Semyung University)
  • 문철 (세명대학교 임상병리학과) ;
  • 김경미 (세명대학교 간호학과)
  • Received : 2010.06.30
  • Accepted : 2010.08.02
  • Published : 2010.08.31

Abstract

Purpose: We tested the inhibitory effect of six purchasable alcohol hand rubs against Staphylococcus aureus. Methods: Five alcohol hand rubs were collected with random manner on the market and 1 alcohol hand rubs which is currently used in a hospital was included. we designed the experimental scheme on the basis of M7-A6 method of NCCLS. Each hand rubs were diluted to 1:1, 1:10, 1:100, 1:1,000 with tryptic soy broth. S. aureus was cultured. Turbidity and the colony counting was measured. Results: With dilution rate of 1:1, all products showed more than 95% of the inhibitory effect on bacterial growth. At 1:10 dilution, product $N^{\circ}1$, 2, 3, 4, and 5 displayed the inhibitory effect of 97.77%, 92.38%, 78.19%, 97.42%, and 96.6%, respectively. But, the inhibitory effect of product $N^{\circ}6$ has been disappeared at that dilution. Over 1:100 dilution, all products lost their inhibitory effect except product $N^{\circ}5$. Product $N^{\circ}5$ displayed more than 96% of inhibitory effect at all dilution rate, even at 1:100 and 1:1,000. Conclusion: We identified that inhibitory effect on bacterial growth of alcohol hand rubs was variable. We suggest that concentration of alcohol should be checked before choosing alcohol hand rubs. Further evaluation of in vivo study is needed.

Keywords

References

  1. Bennett, W., & Lancette, G. A. (1998). Bacteriological Analytical Manual (8th edition, Revision A). Chapter 12. Retrieved May 6, 2009, from http://www.fda.gov/Food/ScienceResearch/LaboratoryMethods/ BacteriologicalAnalyticalManualBAM/UCM071429.
  2. Bollela, V. R., Sato, D. N., & Fonseca, B. A. (1999). McFarland nephelometer as a simple method to estimate the sensitivity of the polymerase polymerase chain reaction using Mycobacterium tuberculosis as a research tool. Brazilian Journal of Medical and Biological Research, 32, 1073-1076. https://doi.org/10.1590/S0100-879X1999000900003
  3. Centers for Disease Control and Prevention. (2002). Guideline for hand hygiene in health-care settings. Morbidity Mortality Weekly Report 51, RR-16.
  4. Cheeseman, K. E., Williams, G. J., Maillard, J. Y., Denyer, S. P., & Mahenthiralingam, E. (2007). Typing of Staphylococcus aureus clinical isolates using random amplification of polymorphic DNA method and comparison to antiseptic susceptibility typing. Journal of Hospital Infection, 67, 388-390. https://doi.org/10.1016/j.jhin.2007.10.003
  5. Chong, Y .S., Lee, K. W., Kim, H. S., Kim, S. M., Yong, D. Y. (2009). Current diagnostic microbiology(4 ed). Seoul: Seo-Heung Choolpansa.
  6. Gaokar, T. A., Geraldo, I., Caraos. L., Modak, S. M. (2005). An alcohol hand rub containing a synergistic combination of an emollient and preservatives: prolonged activity against transient pathogens. Journal of Hospital Infection 59, 12-18. https://doi.org/10.1016/j.jhin.2004.06.030
  7. Jeong, J. S., Choi, J. K., Jeong, I. S., Paek, K. R., In, H. K., Park, K. D. (2007). A Nationwide Survey on the Hand Washing Behavior and Awareness. Journal of Preventive Medicine and Public Health, 40, 197-204. https://doi.org/10.3961/jpmph.2007.40.3.197
  8. Johnson, G. A., English, J. S. (2007). The alcohol hand rub: a good soap substitute? British Journal of Dermatology, 157, 1-3.
  9. Kiem, S. M., & Schentag, J. J. (2006). Relationship of minimal inhibitory concentration and bactericidal activity to efficacy of antibiotics for treatment of ventilator-associated pneumonia. Seminars in Respiratory Critical Care Medicine, 27, 51-67. https://doi.org/10.1055/s-2006-933674
  10. Kim, J. K., & Kim, J. S. (2009). A study on the hand-washing awareness and practices of female university students. Jornal of Food Hygiene and Safety, 24, 128-135.
  11. Korean Society Nosocomial Infection. (2001). Nosocomial infection (2nd ed.). Seoul: Han-mi eui Haak.
  12. Maillard, J. Y. (2007). Bacterial resistance to biocides in the health-care environment: shall we be concerned? Journal of Hospital Infection, 65 (suppl.2), 60-72.
  13. McDonald, P. J., Wetherall, B. L., & Pruul, H. (1981). Postantibiotic leukocyte enhancement: increased susceptibility of bacteria pretreated with antibiotics to activity of leukocytes. Review of Infectious Disease, 3, 38-44. https://doi.org/10.1093/clinids/3.1.38
  14. Mcfarland, J. (1907). Nephelometer: an instrument for estimating the number of bacteria in suspensions used for calculating the opsonic index and for vaccines. Journal of American Medical Association, 14, 1176-1178.
  15. National Committee for Clinical Laboratory Standards. (2003). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically ; Approved standard M7-A6 (6th ed.). Wayne, Pennsylvania, USA.
  16. Odenholt, I. (2001). Pharmacodynamic effects of subinhibitory antibiotic concentrations. International Journal of Antimicrobial Agents, 17, 1-8.
  17. Picheansalthian, W. (2004). A systematic review on the effectiveness of alcohol-based solutions for hand hygiene. International Journal of Nursing Practice, 10, 3-9. https://doi.org/10.1111/j.1440-172X.2003.00457.x
  18. Rotter. (2001). Arguments for alcoholic hand disinfection. Journal of Hospital Infection, 48, S4-S8. https://doi.org/10.1016/S0195-6701(01)90004-0
  19. Rupp, M. E., Fitzgerald, T., Puumala, S., Anderson, J. R., Craig, R., Iwen, P. C., et al. (2008). Prospective, controlled, cross-over trial of alcohol-based hand gel in critical care units. Infection Control and Hospital Epidemiology, 29, 8-15. https://doi.org/10.1086/524333
  20. Widmer, A. F. (2000). Replace hand washing with use of a waterless alcohol hand rub? Clinical Infectious Disease, 31, 136-143. https://doi.org/10.1086/313888