Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지

Anti-Allergic Activities of Ultra-fine Powder from Persimmon

감 초미세 분말의 추출물을 이용한 항산화 및 항아토피 활성

  • Heo, Jin-Chul (Food & Bio-Industry Research Institute and Department of Food Science & Technology, Kyungpook National University) ;
  • Lee, Kang-Yol (Korea Energy Technology Co., Ltd) ;
  • Lee, Beom-Goo (Korea Energy Technology Co., Ltd) ;
  • Choi, So-Young (Gyeongsangbuk-do Agricultural Research & Extension Services) ;
  • Lee, Sook-Hee (Gyeongsangbuk-do Agricultural Research & Extension Services) ;
  • Lee, Sang-Han (Food & Bio-Industry Research Institute and Department of Food Science & Technology, Kyungpook National University)
  • 허진철 (경북대학교 식품생물산업연구소 및 식품공학과) ;
  • 이강열 ((주)한국에너지기술) ;
  • 이범구 ((주)한국에너지기술) ;
  • 최소영 (경북농업기술원) ;
  • 이숙희 (경북농업기술원) ;
  • 이상한 (경북대학교 식품생물산업연구소 및 식품공학과)
  • Published : 2010.02.27
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Abstract

Ultra-fine powder (FA) extracts from persimmon were investigated as potential food materials in industrial processes because such extracts have various useful biological activities. We examined the antioxidant and anti-allergic activity of FA extracts using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing activity of plasma (FRAP) models, and by measuring interleukin 4 (IL-4) expression in spleen cells and in an atopy animal model. FA extracts were prepared using solvents including distilled water and 50% (v/v) acetone. The results showed that FA extracts had potent DPPH and FRAP activities, decreased IL-4 expression in spleen cells, and inhibited ear thickness and inflammation in a mouse animal model. The data indicate that a FA extract could be used as an antioxidant and/or anti-inflammation agent, and in industrial food preparation processes.

초미세 분쇄법은 식품의 가공방법 중 하나로 특히 이를 이용하여 음용으로 활용범위를 넓힐 수 있다. 본 연구는 이에 착안하여 감을 이용한 초미세 분말을 이용하여 항산화 활성과 자가면역질환인 아토피에 효과가 있는지를 확인하여 보았다. 감 초미세 분말을 이용하여 DPPH와 FRAP 항산화 활성 실험을 수행 하였으며, 마우스 동물모델을 이용한 항알러지 피부염 동물모델을 이용하여 확인하였다. 항산화 활성 실험 결과 감 초미세 추출물은 농도에 따른 활성 등가를 나타내었다. DNFB (dinitrofluorobenzene)를 이용한 아토피 유도 후 이의 억제 활성은 아토피 유도군에서 염증의 유도와 함께 약 1.7배 정도 귀의 두께가 증가하는 것을 알 수 있었다. 또한, 감 초미세 추출물 FA 1, 2, 3 추출물을 처리한 결과, FA3이 다른 두 그룹 보다 귀에 비후정도가 감소하는 것으로 나타났다. 또한, 귀의 표피나 진피에서 발견되는 면역관련 세포는 대조군에 비해 아토피를 유도한 군에서 약 7배 정도의 세포수가 증가하는 것을 알 수 있었으며, 감 초미세 분말 추출물을 처리한 경우, 수적으로 감소하는 결과를 얻을 수 있었다.

Keywords

References

  1. Heo, J.C., Chae, J.H., Lee, S.H., Lee, Y.R., Moon, K.D., Chung and S.K., Lee, S.H. (2008) Comparison of radical scavenging and immunomodulatory activities exhibited by an aqueous extract of Diospyros kaki Thunb. fruit (persimmon). Korean J. Food Preserv. 15, 749-753
  2. Bae, S.M., Park, K.J., Shin, D.J., Hwang, Y.I. and Lee, S.C. (2001) Preparation and characterization of jochung with sweet persimmons. J. Korean Soc. Agric. Chem. Biotechnol., 44, 88-91
  3. Bae, S.M., Park, K.J., Kim, J.M., Shin, D.J., Hwang, Y.I. and Lee, S.C. (2002) Preparation and characterization of sweet persimmon wine. J. Korean Soc. Agric. Chem. Biotechnol., 45, 66-70
  4. Kang, W. S. (2004) Application of Nano Technology in Food Industry. Food Engineering Progress, 8(4), 211-223
  5. Keck, C. M. (2004) Challenges and solutions for the delivery of biotech drugs-a review of drug nanocrystal technology and lipid nanoparticles. J. Biotechnol., 113(1-3), 151-170 https://doi.org/10.1016/j.jbiotec.2004.06.007
  6. Desai, M. P., Labhasetwar, V., Amidon, G. L. and Levy, R. J. (1996) Gastrointestinal uptake of biodegradable microparticles: effect of particle size. Pharm. Res., 13, 1838-1845 https://doi.org/10.1023/A:1016085108889
  7. Heo, J.C., Park, J.Y., Hwang, J.S., Park, H.C., Kang, S.W., Hwang, S.J., Yun, C.Y., Kwon, T.K. and Lee, S.H. (2006) Comparison of in vitro antioxidant activity and cyclooxygenase-2 promoter inhibitory activity in Harmonia axyridis Pallas and Coccinella septempunctata Linne. Korean J. Food Preserv., 13, 513-518
  8. Heo, J.C., Park, J.Y., Kwon, T.K., Chung, S.K., Kim, S.U. and Lee, S.H., (2005) Development of high throughput screening techniques using food-borne library against anti-asthma agents. Korean J. Food Preserv., 12, 267-274
  9. Heo, J.C., Nam, S.H., Lee, K.G., Yeo, J.H., Yoon, C.S., Park, C.H., Nam, S.H., Son, M.S., Chung, S.K. and Lee, S.H. (2009) Anti-immunomodulating activities in mycelial filtrates and culture broth of Cordyceps ochraceostromat. Korean J. Food Preserv., 16, 253-258
  10. Yanagisawa, R., Takano, H., Inoue, K., Koike, E., Sadakane, K. and Ichinose, T. (2008) Effects of maternal exposure to di-(2-ethylhexyl) phthalate during fetal and/or neonatal periods on atopic dermatitis in male offspring. Environ. Health Perspect., 116, 1136-1141 https://doi.org/10.1289/ehp.11191
  11. Loizzo, M.R., Said, A., Tundis, R., Hawas, U.W., Rashed, K., Menichini, F., Frega, N.G. and Menichini, F. (2009) Antioxidant and Antiproliferative Activity of Diospyros lotus L. extract and isolated compounds. Plant Foods Hum. Nutr., 64, 264–270
  12. Akagi, T., Ikegami, A., Suzuki, Y., Yoshida, J., Yamada, M., Sato, A. and Yonemori, K. (2009) Expression balances of structural genes in shikimate and flavonoid biosynthesis cause a difference in proanthocyanidin accumulation in persimmon (Diospyros kaki Thunb.) fruit. Planta, 230, 899-915.
  13. Dewanjee, S., Das, A.K., Sahu, R. and Gangopadhyay, M. (2009) Antidiabetic activity of Diospyros peregrina fruit: effect on hyperglycemia, hyperlipidemia and augmented oxidative stress in experimental type 2 diabetes. Food Chem. Toxicol., 47, 2679-2685 https://doi.org/10.1016/j.fct.2009.07.038
  14. Ikegami, A., Akagi, T., Potter, D., Yamada, M., Sato, A., Yonemori, K., Kitajima, A. and Inoue, K. (2009) Molecular identification of 1-Cys peroxiredoxin and anthocyanidin/flavonol 3-O-galactosyltransferase from proanthocyanidin-rich young fruits of persimmon (Diospyros kaki Thunb.). Planta, 230, 841-855 https://doi.org/10.1007/s00425-009-0989-0
  15. Kvarnstrom, M., Jenmalm, M.C. and Ekerfelt, C. (2004) Effect of cryopreservation on expression of Th1 and Th2 cytokines in blood mononuclear cells from patients with different cytokine profiles, analysed with three common assays: an overall decrease of interleukin-4. Cryobiology, 49, 157-168 https://doi.org/10.1016/j.cryobiol.2004.06.003
  16. Schneider, S., Bruns, A., Moewes, B., Holzknecht, B., Hausdorf, G., Riemekasten, G., Radbruch, A., Hiepe, F. and Thiel, A. (2002) Simultaneous cytometric analysis of (auto)antigen-reactive T and B cell proliferation. Immunobiology, 206, 484-495 https://doi.org/10.1078/0171-2985-00196
  17. Kotani, M., Matsumoto, M., Fujita, A., Higa, S., Wang, W., Suemura, M., Kishimoto, T. and Tanaka, T. (2000) Persimmon leaf extract and astragalin inhibit development of dermatitis and IgE elevation in NC/Nga mice. J. Allergy Clin. Immunol., 106, 159-166 https://doi.org/10.1067/mai.2000.107194
  18. Matsumoto, M., Kotani, M., Fujita, A., Higa, S., Kishimoto, T., Suemura, M. and Tanaka, T. (2002) Oral administration of persimmon leaf extract ameliorates skin symptoms and transepidermal water loss in atopic dermatitis model mice, NC/Nga. Br. J. Dermatol., 146, 221-227 https://doi.org/10.1046/j.1365-2133.2002.04557.x
  19. Fukai, S., Tanimoto, S., Maeda, A., Fukuda, H., Okada, Y. and Nomura, M. (2009) Pharmacological activity of compounds extracted from persimmon peel (Diospyros kaki THUNB.). J. Oleo. Sci., 58, 213-219 https://doi.org/10.5650/jos.58.213