Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Identification and Distribution of Bacillus Species in Doenjang by Whole-Cell Protein Patterns and 16S rRNA Gene Sequence Analysis

  • Kim, Tae-Woon (Institute of Life Sciences and Resources, and Graduate School of Biotechnology, Kyung Hee University) ;
  • Kim, Young-Hoon (Institute of Life Sciences and Resources, and Graduate School of Biotechnology, Kyung Hee University) ;
  • Kim, Sung-Eon (Institute of Life Sciences and Resources, and Graduate School of Biotechnology, Kyung Hee University) ;
  • Lee, Jun-Hwa (Institute of Life Sciences and Resources, and Graduate School of Biotechnology, Kyung Hee University) ;
  • Park, Cheon-Seok (Institute of Life Sciences and Resources, and Graduate School of Biotechnology, Kyung Hee University) ;
  • Kim, Hae-Yeong (Institute of Life Sciences and Resources, and Graduate School of Biotechnology, Kyung Hee University)
  • Received : 2010.02.06
  • Accepted : 2010.05.05
  • Published : 2010.08.28
Download PDF
⟨ Previous Next ⟩

Abstract

Many bacteria are involved in the fermentation of doenjang, and Bacillus species are known to perform significant roles. Although SDS-PAGE has been frequently used to classify and identify bacteria in various samples, the microbial diversity in doenjang has not yet been investigated. This study aims to determine the identity and distribution of dominant Bacillus species in doenjang using SDS-PAGE profiles of whole-cell proteins and 16S rRNA gene sequencing. Reference Bacillus strains yielded differential SDS-PAGE banding patterns that could be considered to be highly specific fingerprints. Grouping of bacterial strains isolated from doenjang samples by whole-cell protein patterns was confirmed by analysis of their 16S rRNA gene sequences. B. subtilis was found to be the most dominant strain in most of the samples, whereas B. licheniformis and B. amyloliquefaciens were less frequently found but were also detected in several samples. The results obtained in this study show that a combined identification method using SDS-PAGE profiles of whole-cell proteins and subsequent 16S rRNA gene sequence analysis could successfully identify Bacillus species isolated from doenjang.

Keywords

References

  1. Cho, K. M. and W. T. Seo. 2007. Bacterial diversity in Korean traditional soybean fermented foods (Doenjang and Ganjang) by 16S rRNA gene sequence analysis. Food Sci. Biotechnol. 16: 320-324.
  2. Choi, M. R., H. S. Lim, Y. J. Chung, E. J. Yoo, and J. K. Kim. 1999. Selective cytotoxic effects of doenjang (Korean soybean paste) fermented with Bacillus strains on human liver cell lines. J. Microbiol. Biotechnol. 9: 504-508.
  3. Chung, K. S., J. Y. Kim, S. W. Hong, and B. K. Lee. 2006. Isolation of bacteria producing a B-cell-specific biological response modifier found in Korean fermented soybean paste. J. Microbiol. Biotechnol. 16: 126-135.
  4. Dicks, L. M. T., H. J. J. Van Vuuren, and F. Dellaglio. 1990. Taxonomy of Leuconostoc species, particularly Leuconostoc oenos, as revealed by numerical analysis of total soluble cell protein patterns, DNA base compositions, and DNA-DNA hybridizations. Int. J. Syst. Bacteriol. 40: 83-91. https://doi.org/10.1099/00207713-40-1-83
  5. Ji, W. D., S. H. Yang, M. R. Choi, and J. K. Kim. 1995. Volatile components of Korean soybean paste produced by Bacillus subtilis PM3. J. Microbiol. Biotechnol. 5: 143-148.
  6. Jin, J., S. Y. Kim, Q. Jin, H. Eom, and N. S. Han. 2008. Diversity analysis of lactic acid bacteria in takju, Korean rice wine. J. Microbiol. Biotechnol. 18: 1678-1682.
  7. Kim, D. H., H. P. Song, K. Y. Kim, J. O. Kim, and M. W. Byun. 2004. A correlation between fibrinolytic activity and microflora in Korean fermented soybean products. J. Korean Soc. Food Sci. Nutr. 33: 41-46. https://doi.org/10.3746/jkfn.2004.33.1.041
  8. Kim, T. W., S. H. Jung, J. Y. Lee, S. K. Choi, S. H. Park, J. S. Jo, and H. Y. Kim. 2003. Identification of lactic acid bacteria in kimchi using SDS-PAGE profiles of whole cell proteins. J. Microbiol. Biotechnol. 13: 119-124.
  9. Kim, T. W., J. H. Lee, S. E. Kim, M. H. Park, H. C. Chang, and H. Y. Kim. 2009. Analysis of microbial communities in doenjang, a Korean fermented soybean paste, using nested PCR-denaturing gradient gel electrophoresis. Int. J. Food Microbiol. 131: 265-271. https://doi.org/10.1016/j.ijfoodmicro.2009.03.001
  10. Lee, S. S. 1995. Meju fermentation for a raw material of Korean traditional soy products. Korean J. Mycol. 23: 161-175.
  11. Park, S. H., S. H. Jung, H. J. Kim, Y. H. Chung, and H. Y. Kim. 2006. Classification of meat-based Listeria monocytogenes using whole-cell protein patterns and serotyping analysis. Food Sci. Biotechnol. 15: 324-327.
  12. Pot, B., C. Hertel, W. Ludwig, P. Descheemaeker, K. Kersters, and K. H. Schleifer. 1993. Identification and classification of Lactobacillus acidophilus, L. gasseri, and L. johnsonii strains by SDS-PAGE and rRNA-targeted oligonucleotide probe hybridization. J. Gen. Microbiol. 139: 513-517. https://doi.org/10.1099/00221287-139-3-513
  13. Sanchez, I., S. Sese a, and L. Palop. 2003. Identification of lactic acid bacteria from spontaneous fermentation of 'Almagro' eggplants by SDS-PAGE whole cell protein fingerprinting. Int. J. Food Microbiol. 82: 181-189. https://doi.org/10.1016/S0168-1605(02)00260-X
  14. Shin, S. Y., Y. B. Kim, and T. J. Yu. 1985. Flavor improvement of soybean pastes by the addition of Bacillus licheniformis and Saccharomyces rouxii. Korean J. Food Sci. Technol. 17: 8-14.
  15. Song, B. K., M. M. Clyde, R. Wickneswari, and M. N. Normah. 2000. Genetic relatedness among Lansium domesticum accessions using RAPD markers. Ann. Bot. 86: 299-307. https://doi.org/10.1006/anbo.2000.1186
  16. Xu, D. and J. C. Côté. 2003. Phylogenetic relationships between Bacillus species and related genera inferred from comparision of 3' end 16S rDNA and 5' end 16S-23S ITS nucleotide sequences. Int. J. Syst. Evol. Microbiol. 53: 695-704. https://doi.org/10.1099/ijs.0.02346-0
  17. Yoo, S. K., S. M. Kang, and Y. S. Noh. 2000. Quality properties on soybean pastes made with microorganisms isolated from traditional soybean pastes. Korean J. Food Sci. Technol. 32: 1266-1270.
  18. Yoo, S. K., W. H. Cho, S. M. Kang, and S. H. Lee. 1999. Isolation and identification of microorganisms in Korean traditional soybean paste and soybean sauce. Korean J. Appl. Microbiol. Biotechnol. 27: 113-117.

Cited by

  1. Lantibiotics, Class I Bacteriocins from the Genus Bacillus vol.21, pp.3, 2010, https://doi.org/10.4014/jmb.1010.10017
  2. Screening of Strains with Fibrinolytic Activity and Angiotensin-converting Enzyme Inhibitory Activity from Doenjang vol.21, pp.2, 2010, https://doi.org/10.1007/s10068-012-0074-5
  3. A novel phenicol exporter gene, fexB, found in enterococci of animal origin vol.67, pp.2, 2010, https://doi.org/10.1093/jac/dkr481
  4. Combination of culture-independent and culture-dependent molecular methods for the determination of bacterial community of iru , a fermented Parkia biglobosa seeds vol.3, pp.None, 2010, https://doi.org/10.3389/fmicb.2012.00436
  5. Microbial community analysis of Korean soybean pastes by next-generation sequencing vol.155, pp.1, 2012, https://doi.org/10.1016/j.ijfoodmicro.2012.01.013
  6. Identification of New Delhi Metallo-β-lactamase 1 in Acinetobacter lwoffii of Food Animal Origin vol.7, pp.5, 2010, https://doi.org/10.1371/journal.pone.0037152
  7. Prevalence and Antimicrobial Susceptibility of Foodborne Bacteria in Wild Boars (Sus scrofa) and Wild Deer (Cervus nippon) in Japan vol.10, pp.11, 2010, https://doi.org/10.1089/fpd.2013.1548
  8. Multidrug resistance gene cfr in methicillin-resistant coagulase-negative staphylococci from chickens, ducks, and pigs in China vol.303, pp.2, 2010, https://doi.org/10.1016/j.ijmm.2012.12.004
  9. Subtilomycin: A New Lantibiotic from Bacillus subtilis Strain MMA7 Isolated from the Marine Sponge Haliclona simulans vol.11, pp.6, 2013, https://doi.org/10.3390/md11061878
  10. Reduction of Foodborne Pathogens and Aflatoxins in Doenjang Samples Using Defined Meju vol.34, pp.2, 2010, https://doi.org/10.1111/jfs.12109
  11. High prevalence of Cfr-producing Staphylococcus species in retail meat in Guangzhou, China vol.14, pp.None, 2010, https://doi.org/10.1186/1471-2180-14-151
  12. Epidemiological Characteristics of bla NDM-1 in Enterobacteriaceae and the Acinetobacter calcoaceticus-Acinetobacter baumannii Complex in China from 2011 to 2012 vol.9, pp.12, 2010, https://doi.org/10.1371/journal.pone.0113852
  13. Diversity and functional characterization of microflora isolated from traditional fermented food idli vol.52, pp.11, 2010, https://doi.org/10.1007/s13197-015-1791-6
  14. Characterization of a novel small plasmid carrying the florfenicol resistance gene floR in Haemophilus parasuis vol.70, pp.11, 2015, https://doi.org/10.1093/jac/dkv230
  15. Determination of biogenic amines and total aflatoxins: Quality index of starter culture soy sauce samples vol.25, pp.4, 2010, https://doi.org/10.1007/s10068-016-0194-4
  16. Functional Characterization of Bacterial Communities Responsible for Fermentation of Doenjang : A Traditional Korean Fermented Soybean Paste vol.7, pp.None, 2016, https://doi.org/10.3389/fmicb.2016.00827
  17. Potentiality of Bacillus amyloliquefaciens KFCC11574P isolated from Korean traditional doenjang as a starter in the production of functional soya bean paste vol.51, pp.1, 2010, https://doi.org/10.1111/ijfs.12973
  18. Characterization of a cfr-Carrying Plasmid from Porcine Escherichia coli That Closely Resembles Plasmid pEA3 from the Plant Pathogen Erwinia amylovora vol.60, pp.1, 2010, https://doi.org/10.1128/aac.02114-15
  19. Presence of VIM-Positive Pseudomonas Species in Chickens and Their Surrounding Environment vol.61, pp.7, 2017, https://doi.org/10.1128/aac.00167-17
  20. Salad dressing spoilage by Bacillus amyloliquefaciens with gas formation vol.21, pp.None, 2018, https://doi.org/10.1590/1981-6723.2517
  21. Emergence of IncX3 Plasmid-Harboring blaNDM-5 Dominated by Escherichia coli ST48 in a Goose Farm in Jiangsu, China vol.10, pp.None, 2010, https://doi.org/10.3389/fmicb.2019.02002
  22. Characterization of a bla IMP-4 -carrying plasmid from Enterobacter cloacae of swine origin vol.74, pp.7, 2010, https://doi.org/10.1093/jac/dkz107
  23. Characterization of a bla NDM-1 -carrying IncHI5 plasmid from Enterobacter cloacae complex of food-producing animal origin vol.75, pp.5, 2010, https://doi.org/10.1093/jac/dkaa010
  24. Diversity and enzymatic potential of thermophilic bacteria associated with terrestrial hot springs in Algeria vol.51, pp.4, 2020, https://doi.org/10.1007/s42770-020-00376-0
  25. Rapid Increase in the IS 26 -Mediated cfr Gene in E. coli Isolates with IncP and IncX4 Plasmids and Co-Existing cfr and mcr-1 Genes in a Swine Farm vol.10, pp.1, 2010, https://doi.org/10.3390/pathogens10010033
  26. A Natural Antimicrobial Agent: Analysis of Antibacterial Effect and Mechanism of Compound Phenolic Acid on Escherichia coli Based on Tandem Mass Tag Proteomics vol.12, pp.None, 2010, https://doi.org/10.3389/fmicb.2021.738896
  27. Microbial diversity and function of soybean paste in East Asia: what we know and what we don’t vol.37, pp.None, 2010, https://doi.org/10.1016/j.cofs.2020.10.012
  28. Dissemination of the tet(X)-Variant Genes from Layer Farms to Manure-Receiving Soil and Corresponding Lettuce vol.55, pp.3, 2010, https://doi.org/10.1021/acs.est.0c05042
  29. First detection of the multiresistance gene cfr in Escherichia coli from retail vegetables, China vol.57, pp.5, 2010, https://doi.org/10.1016/j.ijantimicag.2021.106348
  30. Biodegradation of azo dye-containing wastewater by activated sludge: a critical review vol.37, pp.6, 2010, https://doi.org/10.1007/s11274-021-03067-6
  31. Multiple Mechanisms of Tigecycline Resistance in Enterobacteriaceae from a Pig Farm, China vol.9, pp.2, 2010, https://doi.org/10.1128/spectrum.00416-21
  32. Characterization of NDM-1-Producing Carbapenemase in Proteus mirabilis among Broilers in China vol.9, pp.12, 2010, https://doi.org/10.3390/microorganisms9122443