Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Chelation of Calcium Ions by Poly(${\gamma}$-Glutamic Acid) from Bacillus subtilis (Chungkookjang)

  • Tsujimoto, Takashi (Department of Applied Chemistry, Graduate School of Engineering, Osaka University) ;
  • Kimura, Junya (Department of Applied Chemistry, Graduate School of Engineering, Osaka University) ;
  • Takeuchi, Yasushi (Department of Applied Chemistry, Graduate School of Engineering, Osaka University) ;
  • Uyama, Hiroshi (Department of Applied Chemistry, Graduate School of Engineering, Osaka University) ;
  • Park, Chung (BioLeaders Corporation) ;
  • Sung, Moon-Hee (BioLeaders Corporation)
  • Received : 2010.04.30
  • Accepted : 2010.07.06
  • Published : 2010.10.28
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Abstract

Many studies have clarified that poly(${\gamma}$-glutamic acid) (PGA) increases the solubility of $Ca^{2+}$, suggesting that PGA enhances calcium absorption in the small intestine. However, there has been no report on the specific interaction between PGA and $Ca^{2+}$ in water. We studied the aqueous solution properties of PGA calcium salt (PGA-Ca complex). The chelating ability and binding strength of PGA for $Ca^{2+}$ were evaluated. The PGA-Ca complex was soluble in water, in contrast to the insolubility of poly(acrylic acid) (PAA) calcium salt, and the chelating ability of PGA for $Ca^{2+}$ was almost the same as that of PAA. The globular conformation of the PGA-Ca complex in water was estimated by SEC and viscosity measurements. The chelation ability of PGA for $Ca^{2+}$ was examined by $^1H$ NMR. The present study showing the characteristics of the PGA-Ca complex will provide useful information about the calcium absorption by PGA in vivo.

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References

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