Journal of Soil and Groundwater Environment (한국지하수토양환경학회지:지하수토양환경)

Korean Society of Soil and Groundwater Environment (한국지하수토양환경학회)
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Effects of Short-Term Oxygen Exposure on Anaerobic Reductive Dechlorination and Formate Fermentation by Evanite Culture

혐기성탈염소화 혼합균주에서 산소 노출이 탈염소화 및 수소발생 발효에 미치는 영향

  • 홍의전 (고려대학교 대학원 환경공학과) ;
  • 박선화 (고려대학교 환경기술정책연구소) ;
  • 임종환 (고려대학교 대학원 환경공학과) ;
  • 안홍일 (SK에너지(주) 환경사업개발부) ;
  • 김남희 (SK에너지(주) 환경사업개발부) ;
  • 이석우 (SK에너지(주) 환경사업개발부) ;
  • 김영 (고려대학교 대학원 환경공학과)
  • Received : 2010.11.09
  • Accepted : 2010.12.28
  • Published : 2010.12.31
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Abstract

Oxygen sensitivity and substrate requirement have been known as possible reasons for the intricate growth of Dehalococcoides spp. and limiting factors of for routinely applying bioaugmentation using anaerobic Dehalococcoides-containing microbes for remediating chlorinated organic compounds. To explore the effect of the short-term exposure of the short-term exposure of oxygen on Dehalococcoides capability, dechlorination performance, and hydrogen production fermentation from formate, an anaerobic reductive dechlorination mixed-culture (Evanite culture) including dehalococcoides spp. was in this study. In the results, once the mixed-culture were exposed to oxygen, trichloroethylene (TCE) degradation rate decreased and it was not fully recovered even addition of excess formate for 40 days. In contrast, hydrogen was continuously produced by hydrogen-fermentation process even under oxygen presence. The results indicate that although the oxygen-exposed cells cannot completely dechlorinate TCE to ethylene (ETH), hydrogen fermentation process was not affected by oxygen presence. These results suggest that dechlorinating microbes may more sensitive to oxygen than fermenting microbes, and monitoring dechlorinators activity may be critical to achieve an successful remediation of a TCE contaminated-aquifer through bioaugmentation using Dehalococcoides spp..

Keywords

References

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