The Wound Healing Effect of PDRN(polydeoxyribonucleotide) Material on Full Thickness Skin Defect in the Mouse

흰 쥐의 전층피부 결손에 대한 PDRN (polydeoxyribonucleotide) 제재의 창상치유 효과

  • Kim, Yo-Han (Department of Plastic and Reconstructive Surgery, Eulji General Hospital, College of Medicine, Eulji University) ;
  • Lee, Jong-Hoon (Department of Plastic and Reconstructive Surgery, Eulji General Hospital, College of Medicine, Eulji University) ;
  • Min, Kyung-Hee (Department of Plastic and Reconstructive Surgery, Eulji General Hospital, College of Medicine, Eulji University) ;
  • Hong, Sung-Hee (Department of Plastic and Reconstructive Surgery, Eulji General Hospital, College of Medicine, Eulji University) ;
  • Lee, Won-Mi (Department of Pathology, Eulji General Hospital, College of Medicine, Eulji University) ;
  • Jun, Jin-Hyun (Department of Bio-Medical Laboratory Science, College of Health Science, Eulji University)
  • 김요한 (을지대학교 의과대학 을지병원 성형외과학교실) ;
  • 이종훈 (을지대학교 의과대학 을지병원 성형외과학교실) ;
  • 민경희 (을지대학교 의과대학 을지병원 성형외과학교실) ;
  • 홍성희 (을지대학교 의과대학 을지병원 성형외과학교실) ;
  • 이원미 (을지대학교 의과대학 을지병원 병리학과교실) ;
  • 전진현 (을지대학교 보건과학대학 임상병리학과)
  • Received : 2009.11.26
  • Accepted : 2010.03.23
  • Published : 2010.05.10

Abstract

Purpose: Many topical agents had been used for burn or wound treatment. An awareness of topical agents on various aspects of wound healing permits the clinician to choose the most appropriate material to advantageously control the wound process and final results. Although polydeoxyribonucleotide (PDRN) was used as a tissue repair stimulating agent in a number of human diseases, such as ulcers and burns, its wound healing effects were largely unreported. We aimed to compare the woundhealing effects of PDRN and common dressing materials on full-thickness skin defect in the mouse. Methods: Full-thickness skin defects were made on the back of mice (N=60). The mice were divided into the following 4 groups according to the dressing used for the wounds: group O (Polydeoxyribonucleotide cream), group I (Polydeoxyribonucleotide solution), group M (Medifoam$^{(R)}$), and group G (dry gauze, control group). We analyzed the gross findings, wound sizes and histological findings for the groups. Results: The rate of wound size was decreased in order of group I, group O, group M and group G. The histological findings revealed that the I group showed more reepithelialization and granulation tissue formation and less inflammatory cell infiltration than the other materials. The grade score of wound healing was increased in order of group I, group O, group M and group G. Conclusion: PDRN applicated wound dressings can be used for treating a full-thickness skin defect wounds. Considering its superior efficacy in comparison to the efficacies of other wound dressings, PDRN soaked gauze dressing should be preferentially used for the treatment of fullthickness skin wounds.

References

  1. Galeano M, Bitto A, Altavilla D, Minutoli L, Polito F, Calò M, Lo Cascio P, Stagno d'Alcontres F, Squadrito F: Polydeoxyribonucleotide stimulates angiogenesis and wound healing in the genetically diabetic mouse. Wound Repair Regen 16: 208, 2008 https://doi.org/10.1111/j.1524-475X.2008.00361.x
  2. Cavallini M: Biorevitalization and cosmetic surgery of the face: synergies of action. J Appl Cosmetol 22: 125, 2004
  3. Victor-Vega C, Desai A, Monesinos MC, Cronstein BN: Adenosine A2A receptor agonists promote more rapid wound healing than recombinant human platelet-derived growth factor (Becaplermin gel). Inflammation 26: 19, 2002 https://doi.org/10.1023/A:1014417728325
  4. Montesinos MC, Desai A, Chen JF, Yee H, Schwarzschild MA, Fink JS, Cronstein BN: Adenosine promotes wound healing and mediates angiogenesis in response to tissue injury via occupancy of A (2A) receptors. Am J Pathol 160: 2009, 2002 https://doi.org/10.1016/S0002-9440(10)61151-0
  5. Yamaoka T, Kondo M, Honda S, Iwahana H, Moritani M, Ii S, Yoshimoto K, Itakura M: Amidophosphoribosyltransferase limits the rate of cell growth-linked de novo purine biosynthesis in the presence of constant capacity of salvage purine biosynthesis. J Biol Chem 272: 17719, 1997 https://doi.org/10.1074/jbc.272.28.17719
  6. Carver JD: Dietary nucleotides: cellular immune, intestinal and hepatic system effects. J Nutr 124: 144S, 1994 https://doi.org/10.1093/jn/124.suppl_1.144S
  7. Abbracchio MP, Burnstock G: Purinergic signalling: Pathophysiological roles. Jpn J Pharmacol 78: 113, 1998
  8. Valdatta L, Thione A, Mortarino C, Buoro M, Tuinder S: Evaluation of the efficacy of polydeoxyribonucleotides in the healing process of autologous skin graft donor sites: A pilot study. Curr Med Res Op 20: 403, 2004 https://doi.org/10.1185/030079904125003116
  9. De Aloe G, Rubegni P, Biagioli M, Taddeucci P, Fimiani M: Skin graft donor site and use of polydeoxyribonucleotide as a treatment for skin regeneration: A randomized, controlled, double-blind, clinical trial. Wounds 16: 1, 2004
  10. Bothwell MH, Wilson WH, Shooter EM: The relationship between glandular kallilrein and growth factor-processing of mouse submaxillary gland. J Biol Chem 254: 7287, 1979
  11. Krawczyk WS: A pattern of epidermal cell migration during wound healing. J Cell Biol 49: 247, 1971 https://doi.org/10.1083/jcb.49.2.247
  12. Reimer K, Wichelhaus TA, Schafer V, Rudolph P, Kramer A, Wutzler P, Ganzer D, Fleischer W: Antimicrobial effectiveness of povidone-iodine and consequences for new application areas. Dermatology 204 Suppl 1: 114, 2002 https://doi.org/10.1159/000057738
  13. Rubegni P, De Aloe G, Mazzatenta C, Cattarini L, Fimiani M: Clinical evaluation of the trophic effect of polydeoxyribonucleotide (PDRN) in patients undergoing skin explants. A pilot study. Curr Med Res Opin 17: 128, 2001 https://doi.org/10.1185/0300799039117047
  14. Bitto A, Galeano M, Squadrito F, Minutoli L, Polito F, Dye JF, Clayton EA, Calò M, Venuti FS, Vaccaro M, Altavilla D: Polydeoxyribonucleotide improves angiogenesis and wound healing in experimental thermal injury. Crit Care Med 36: 1594, 2008 https://doi.org/10.1097/CCM.0b013e318170ab5c
  15. Sini P, Denti A, Cattarini G, Daglio M, Tira ME, Balduini C: Effect of polydeoxyribonucleotides on human fibroblasts in primary culture. Cell Biochem Funct 17: 107, 1999 https://doi.org/10.1002/(SICI)1099-0844(199906)17:2<107::AID-CBF815>3.0.CO;2-#