Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Defatted Grape Seed Extracts Suppress Adipogenesis in 3T3-L1 Preadipocytes

포도씨 탈지박 추출물 처리가 3T3-L1 Preadipocyte 내 지방 생성에 미치는 영향

  • Cho, Young-Min (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Lee, Seon-Mi (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Kim, Young-Hwa (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Jeon, Geon-Uk (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Sung, Jee-Hy (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Jeong, Heon-Sang (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Lee, Jun-Soo (Dept. of Food Science and Technology, Chungbuk National University)
  • Received : 2010.03.02
  • Accepted : 2010.04.09
  • Published : 2010.06.30
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Abstract

The objective of this study was to evaluate the effect of defatted grape seed extract (DGSE) on adipocyte differentiation in 3T3-L1 preadipocytes. DGSE at 100 ${\mu}g$/mL significantly suppressed lipid accumulation and glycerol-3-phosphate dehydrogenase activity in hormonally stimulated adipocytes, an indicator of adipocyte differentiation. In order to understand the anti-adipogenic effects of DGSE, the changes in the expression of several adipogenic transcription factors including peroxisome proliferator-activated receptor (PPAR) $\gamma$, CCAAT/enhancer-binding protein (C/EBP) $\alpha$ and $\beta$ were investigated using immunoblotting. DGSE suppressed the expression of PPAR$\gamma$, C/EBP$\alpha$, and C/EBP$\beta$ proteins compared with control adipocytes in a dose-dependent manner. This results indicated that DGSE may alter fat mass by directly affecting adipogensis in maturing preadipocytes and thus may have applications for the treatment of obesity.

본 연구에서는 농산 가공 폐기물로 버려지고 있는 포도씨 탈지박 methanol 추출물의 항비만 활성을 확인하고자 하였다. DGSE에 의한 TG 생성 저해효과를 측정하기 위해 시료를 3T3-L1 preadipocyte에 농도별(0, 25, 50, 100, 200 ${\mu}g/mL$)로 처리하면서 분화를 유도하였다. 실험 결과 DGSE는 지방세포내의 TG 생성과 GPDH 활성 및 주요 전사 인자인 PPAR$\gamma$ 및 C/EBP $\alpha$와 $\beta$의 발현을 농도 의존적으로 억제하는 것으로 나타났다. DGSE는 100 ${\mu}g/mL$의 비교적 낮은 농도에서도 우수한 지방 분화억제 활성을 나타내어 경제적이며 효과적인 항비만 기능성식품으로서의 활용가치가 기대된다.

Keywords

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