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Development of Species-Specific Primers for PCR Identification of Lactobacillus hilgardii and Lactobacillus farciminis in Kimchi

  • Received : 2010.05.17
  • Accepted : 2010.06.16
  • Published : 2010.06.30

Abstract

The aim of this study was to develop species-specific primer sets for kimchi Lactobacillus. Known gene sequences of Lactobacillus 16S rRNA were collected from the NCBI Gene bank, and 69 primer sets were designed using the homologous gene sequence. Six species of kimchi Lactobacilli were used as reference strains: Lactobacillus brevis KCTC3102, Lactobacillus farciminis KCTC3681, Lactobacillus fermentum KCTC3112, Lactobacillus hilgardii KCTC3500, Lactobacillus plantarum KCTC3099, and Lactobacillus sanfranciscensis KCTC3205. PCR amplification and gel electrophoresis were performed to identify the accuracy and specificity of the developed primer set. The results show that the primer set of 5'-aagcctgcgaaggcaag-3' & 5'-aggccaccggctttg-3', 5'-acatactatgcaaatctaagagattagacg-3' & 5'-actgagaatggctttaagagattagcttac-3' resulted in a specific PCR band on L. hilgardii, and primer set of 5'-ctaataccgcataacaactactttcacat-3' & 5'-aacttaataaaccgcctacattctctttac-3' on L. farciminis. The results indicate that the developed primer sets can provide a useful tool for the identification and differentiation of L. hilgardii and L. farciminis from other Lactobacillus species of kimchi.

Keywords

References

  1. Bottazzi V. 1988. An introduction to rod-shaped lacticacid bacteria. Biochimie 70: 303-315. https://doi.org/10.1016/0300-9084(88)90203-9
  2. Stiles ME, Holzapfel WH. 1997. Lactic acid bacteria of foods and their current taxonomy. Int J Food Microbiol 36: 1-29. https://doi.org/10.1016/S0168-1605(96)01233-0
  3. Axelsson L. 1998. Classification and physiology. In Lactic Acid Bacteria: Microbiology and Functional Aspects. Salminen S, Wright AV, eds. 2nd ed. Marcel Dekker, New York, USA. p 1-72.
  4. Caplice E, Fitzgerald GF. 1999. Food fermentations: role of microorganisms in food production and preservation. Int J Food Microbiol 50: 131-149. https://doi.org/10.1016/S0168-1605(99)00082-3
  5. Leroy F, De Vuyst L. 2004. Lactic acid bacteria as functional starter cultures for the food fermentation industry. Trends Food Sci Technol 15: 67-78. https://doi.org/10.1016/j.tifs.2003.09.004
  6. Drider D, Fimland G, Hechard Y, Mcmullen LM, PrevostH. 2006. The continuing story of class IIa bacteriocins. Microbiol Mol Biol Rev 70: 564-582. https://doi.org/10.1128/MMBR.00016-05
  7. Ballongue J. 1998. Bifidobacteria and probiotic action. In Lactic Acid Bacteria: Microbiology and Functional Aspects. Salminen S, Wright AV, eds. 2nd ed. Marcel Dekker, New York, USA. p 519-587.
  8. Shah N. 2000. Probiotic bacteria: Selective enumeration and survival in dairy foods. J Dairy Sci 83: 1-14. https://doi.org/10.3168/jds.S0022-0302(00)74847-8
  9. Shah NP, Wu C. 1999. Aflatoxin B1 binding abilities of probiotic bacteria. Biosci Microflora 18: 43-48. https://doi.org/10.12938/bifidus1996.18.43
  10. Kandler O, Weiss N. 1986. Genus Lactobacillus Beijerinck 1901. 212A-L. In Bergey’s Manual of Systematic Bacteriology. Sneath PHA, Mair NC, Sharpe ME, Holt JG, eds. 2nd ed. Williams and Wilkins Co., Baltimore, USA.p 1209-1234.
  11. Mheen TI, Kwon TW. 1984. Effect of temperature and salt concentration on kimchi fermentation. Korean J FoodSci Technol 16: 443-450.
  12. Lee CW, Ko CY, Ha DM. 1992. Microfloral changes of the lactic acid bacteria during kimchi fermentation andidentification of the isolates. Kor J Microbiol Biotechnol20: 102-109.
  13. Cheigh HS, Park KY. 1994. Biochemical, microbiological and nutritional aspect of kimchi. Food Sci Nutr 34:175-203.
  14. Han HU, Lim CR, Park HK. 1990. Determination of microbial community as an indicator of kimchi fermentation.Korean J Food Sci Technol 22: 26-32.
  15. Cho JS. 1991. Changes of microflora in commercial kimchi.Korean J Dietary Culture 6: 479-501
  16. Lee CJ, Park HW, Kim KY. 1998. The book of Kimchi. Korean Overseas Culture and Information Service, Seoul, Korea. p 93-95.
  17. Lee MK, Jang DJ, Kim YJ, Kim DS, Kim SH, Yang HJ.2009. Development of Kimchi microbes resources and local type for kimchi globalization. In Studies for globalization of top 5 Korean traditional foods. 1st ed. Annual Research Paper of Korean Food Research Institute. p 63-102.
  18. So MH, Kim YB. 1995. Identification of psychrotrophic lactic acid bacteriaisolated from kimchi. Korean J Food Sci Technol 27: 495-505.
  19. Jo JS, You HY, Kim YM. 1993. Recent trends and new developments of traditional fermented food industry.Bioindustry News 6: 11-15.
  20. Ait-Belgnaoui A, Han W, Lamine F, Eutamene H, FioramontiJ, Bueno L, Theodorou V. 2006. Lactobacillus farciminis treatment suppresses stress induced visceral hypersensitivity:a possible action through interaction with epithelial cell cytoskeleton contraction. Gut 55: 1090-1094. https://doi.org/10.1136/gut.2005.084194
  21. Yoon JH, Kang SS, Mheen TI, Ahn JS, Lee HJ, Kim TK,Park CS, Kho YH, Kang KH, Park YH. 2002. Lactobacillus kimchii sp. nov., a new species from kimchi. Int J Sys Evol Microbiol 50: 1789-1795.
  22. Obrietan K, Gao XB, van den Pol AN. 2002. Excitatory actions of GABA increase BDNF expression via a MAPKCREB-dependent mechanism-a positive feedback circuit in developing neurons. J Neurophysiol 88: 1005-1015. https://doi.org/10.1152/jn.2002.88.2.1005
  23. LoTurco JJ, Owens DF, Heath MJ, Davis MB, KriegsteinAR. 1995. GABA and glutamate depolarize cortical progenitor cells and inhibit DNA synthesis. Neuron 15: 1287-1298. https://doi.org/10.1016/0896-6273(95)90008-X
  24. Haydar TF, Wang F, Schwartz ML, Rakic P. 2000. Differential modulation of proliferation in the neocortical ventricular and subventricular zones. J Neurosci 20: 5764-5774.
  25. Shinji H. 2002. Novel physiological functions of GABA and its application (Okinawa Prefecture. S., Japan Science and Technology Corporation. S). Dai1kai Chiiki kara Hasshin suru Kagaku Gijutsu Shinpojiumu Yokoshu. Heisei14 Nendo. Sangakukan no Renkei to Kenko Baio Bijinesu no Soshutsu S: 37-42
  26. Choi JY, Kim M, Lee JH. 2002. Reevaluation of the change of Leuconostoc species and Lactobacillus plantarum by PCR during Kimchi fermentation. J MicrobiolBiotechnol 12: 166-171.
  27. Kim TW, Min SG, Choi DH, Jo JS, Kim HY. 2000. Rapid identification of Lactobacillus plantarum in Kimchi using polymerase chain reaction. J Microbiol Biotechnol 10: 881-884.
  28. Park JA, Heo GY, Lee JS, Oh YJ, Kim BY, Min TI, KimCK, Ahn JS. 2003. Change of microbial communities in Kimchi fermentation at low temperature. Kor J Microbiol 39: 45-50.

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