Journal of Microbiology and Biotechnology

  • 제20권3호
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  • Pages.494-500
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  • 2010
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Identification, Fermentation, and Bioactivity Against Xanthomonas oryzae of Antimicrobial Metabolites Isolated from Phomopsis longicolla S1B4

  • Lim, Chae-Sung (Division of Bioscience and Biotechnology, Bio/Molecular Informatics Center Konkuk University) ;
  • Kim, Ji-Young (Division of Bioscience and Biotechnology, Bio/Molecular Informatics Center Konkuk University) ;
  • Choi, Jung-Nam (Division of Bioscience and Biotechnology, Bio/Molecular Informatics Center Konkuk University) ;
  • Ponnusamy, Kannan (Division of Bioscience and Biotechnology, Bio/Molecular Informatics Center Konkuk University) ;
  • Jeon, Yul-Taek (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Kim, Soo-Un (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Kim, Jeong-Gu (Genomics Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA)) ;
  • Lee, Choong-Hwan (Division of Bioscience and Biotechnology, Bio/Molecular Informatics Center Konkuk University)
  • 투고 : 2009.09.17
  • 심사 : 2009.10.06
  • 발행 : 2010.03.31
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초록

Bacterial blight, an important and potentially destructive bacterial disease in rice, is caused by Xanthomonas oryzae. Recently, this organism has developed resistance to available antibiotics, prompting scientists to find a suitable alternative. This study focused on secondary metabolites of Phomopsis longicolla to target X. oryzae. Five bioactive compounds were isolated by activity-guided fractionation from ethyl acetate extracts of mycelia and were identified by LC/MS and NMR spectroscopy as dicerandrol A, dicerandrol B, dicerandrol C, deacetylphomoxanthone B, and fusaristatin A. This is the first time fusaristatin A has been isolated from Phomopsis sp. Deacetylphomoxanthone B showed a higher antibacterial effect against X. oryzae KACC 10331 than the positive control (2,4-diacetyphloroglucinol). Dicerandrol A also showed high antimicrobial activity against Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis) and yeast (Candida albicans). In addition, high production yields of these compounds were obtained at the stationary and death phases.

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참고문헌

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