한국토양비료학회지 (Korean Journal of Soil Science and Fertilizer)

  • 제43권5호
  • /
  • Pages.659-666
  • /
  • 2010
  • /
  • 0367-6315(pISSN)
  • /
  • 2288-2162(eISSN)
한국토양비료학회 (Korean Society of Soil Science and Fertilizer)
권호 표지

Lysobacter antibioticus HS124를 이용한 배추좀나방 (Plutella xylostella L.)의 생물학적 방제

Biological Control of Diamondback Moth (Plutella xylostella L.) by Lysobacter antibioticus HS124

  • 강성준 (전남대학교 농업생명과학대학) ;
  • 이용성 (전남대학교 농업생명과학대학) ;
  • 이소연 (전남대학교 농업생명과학대학) ;
  • 윤근영 (전남대학교 농업생명과학대학) ;
  • 홍성현 (전남대학교 농업생명과학대학) ;
  • 박윤석 ((주) 푸르네) ;
  • 김익수 (전남대학교 농업생명과학대학) ;
  • 박노동 (전남대학교 농업생명과학대학) ;
  • 김길용 (전남대학교 농업생명과학대학)
  • 투고 : 2010.09.14
  • 심사 : 2010.10.21
  • 발행 : 2010.10.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

선행연구에서 근권 토양으로부터 분리된 Lysobacter antibioticus HS124 (HS124)는 lytic enzyme으로써 chitinase, gelatinase, lipase 및 protease 등의 효소와 항생물질인 4-hydroxyphenylacetic acid (4-HPAA)를 생성하였다. 본 실험에서는 HS124를 이용하여 배추좀나방 (diamondback moth, Plutella xylostella L.) 3~4령 유충의 살충활성을 검정하였다. HS124 배양액을 배추좀나방 유충에 처리하였을 때 유충은 파괴되어 분해되었다. HS124가 생성하는 4-HPAA를 유충에 처리하였을 때 처리 농도가 높을수록 살충율은 증가하였으며, HS124 배양액에 Tween 80을 첨가하였을 때 첨가하지 않은 처리구보다 살충율이 1.4배 높았다. 한편 화학 살충제 (IS), HS124 배양액 (HS124), 식물추출물 (매직파이; MP), HS124 배양액+식물추출물 (HS124+MP) 및 멸균수 (SDW)를 이용하여 배추좀나방 유충의 살충율을 검정하였다. HS124+MP 처리구에서 가장 높은 살충율을 나타내었고, IS, MP, HS124 및 SDW 처리구 순으로 살충율이 감소하였다. HS124 처리구는 대조구인 멸균수처리구보다 31% 높은 살충율을 나타내었고, HS124+MP처리구 보다 40% 낮은 살충율을 나타내었다. 이러한 결과로 보아 항생물질과 다양한 lytic enzyme을 생성하는 L. antibioticus HS124 배양액과 식물추출물의 혼합제제는 배추좀나방의 생물학적 방제제로써 가치가있다고 사료된다.

Lysobacter antibioticus HS124 was isolated from rhizosphere soil in previous experiments, which produced lytic enzymes such as chitinase, gelatinase, lipase and protease. In addition, HS124 released an antibiotic compound, 4-hydroxyphenylacetic acid (4-HPAA). When larvae of P. xylostella was treated with HS124 culture broth, its body was destroyed, and degraded with the increase of incubation time, yielding glycine which was detected from HS124 culture broth. When 4-HPAA produced from HS124 was sprayed, larvae mortality increased with increasing concentration of 4-HPAA. When HS124 culture supplemented with Tween 80 was sprayed, its insecticidal activity against larvae was approximately 1.4 times higher compared to the culture without Tween 80. Insecticide (IS), HS124 culture broth (HS124), Magic-pi (MP) and HS124 culture broth+Magic-pi (HS124+MP) were each treated against larvae of P. xylostella to investigate their insecticidal effect where sterile diluted water (SDW) was used as a control. The highest mortality of larvae was found in HS124+MP, followed by IS, MP, HS124 and SDW respectively. Mortality of larvae in HS124 was 31% higher than that in SDW, but 41% lower than that in HS124+MP, meaning that both enzymes and antibiotics produced from HS124 may synergistically act as active agents with plant extract containing neem oil and turmeric in HS124+MP treatment. These results suggested that L. antibioticus HS124 together with plant extract can be one of candidates for biocontrol agents against Plutella xylostella.

키워드

참고문헌

  1. Ankersmit, G.W. 1953. DDT resistance in Plutella maculipennis (Curt.) (Lepidoptera) in Java. Bull. Entomol. Res. 44:421-425. https://doi.org/10.1017/S0007485300025530
  2. Butterworth, J.H. and E.D. Morgan. 1968. Isolation of a substance that suppresses feeding in the locusts. Chem. Commun. 1:23-24.
  3. Cho, Y.S. and S.C. Lee. 1994. Resistance development and Cross-resistance of diamondback moth (Lepidoptera: Plutellidae) by single selection of several insecticides. Korea J. Pest. Sci. 33:242-249.
  4. Christensen, P. and D. Cook. 1978. Lysobacter, a new genus of non-fruiting, gliding bacteria with a high base ratio (soil and water organisms). Int. J. Syst. Bacteriol. 28:367-393. https://doi.org/10.1099/00207713-28-3-367
  5. Cox, P.A. and O.W. Sacks. 2002. Sacks, Cycad neurotoxins, consumption of flying foxes, and ALS-PDC disease in Guam. Neurology. 58:956-959. https://doi.org/10.1212/WNL.58.6.956
  6. Damare, S., C. Raghukumar, U.D. Muraleedharan, and S. Raghukumar. 2006. Deep-sea fungi as a source of alkaline and cold-tolerant proteases. Enzyme Microb. Technol. 39:172-181. https://doi.org/10.1016/j.enzmictec.2006.03.032
  7. Eliana, T.I., V.P. Geni, T.M. Dalva, H.P. Maria, and M.O.J.N. Pedro. 2007. Production of extra-cellular protease by a Brazilian Strain of Beauveria bassiana reactivated on Coffee Berry Borer, Hypothenemus hampei. Braz. Arch. Biol. Technol. 50:217-223.
  8. Evans, J.R., E.J. Napier, and R.A. Fletton. 1978. New quinoline compound isolated from the fermentation broth of Cytophaga johnsonii. J. Antibiot. 31:952-958. https://doi.org/10.7164/antibiotics.31.952
  9. Fallowfield, H.J. and M.J. Daft. 1988. The extra-cellular release of dissolved organic carbon by freshwater Cyanobacteria and algae and the interaction with Lysobacter CP-1. Br. Phycol. J. 23:317-326. https://doi.org/10.1080/00071618800650351
  10. Graciela, B., B.J.E. Lozbetez-Meza, G. Cozzi, C.F. Piccinetti, and J.E. Ibarra. 2000. Characterization of INTA 51-3, a new atypical strain of Bacillus thuringiensis from Argentina. Microbiol. 41:369-401.
  11. Han, K.H. and S.D. Kim. 1999. Selection and identification of Promicromonospora sp. KH-28 producing chitinase and antifungal antibiotic. Kor. J. Appl. Microbiol. Biotechnol. 27:191-196.
  12. Hashidoko, Y., T. Nakayama, Y. Homma, and S. Tahara. 1999. Structure elucidation of xanthobaccin A, a new antibiotic produced from Stenotrophomonas sp. strain SB-K88. Tetrahedron Lett. 40:2957-2960. https://doi.org/10.1016/S0040-4039(99)00336-6
  13. Hepburn, H.R. 1985. Structure of the integument. In Comprehensive Insect Physiology Biochemistry and Pharmacology. eds. by Kerkut, G.A. and Gilbert, L.I. Integument, Res. Circ. Pergamon Press, Oxford, 3:1–8.
  14. Hwang, I.C., J. Kim, H.M. Kim, D. I. Kim, S.G. Kim, S.S. Kim, and C. Jang. 2009. Evaluation of toxicity of plant extract made by Neem and Matrine against main pests and natural enemies. Korean J. Appl. Entomol. 48:87-94. https://doi.org/10.5656/KSAE.2009.48.1.087
  15. Ishiwata, S. 1901. On a kind of sever flacherie (sotto disease) Dainihon Sanshi Kaiho. 9:1-5.
  16. Jeong, H.U., H.H. Im, S.K. Chang, C.H. Paik, T.H. Han, I.S. Kim, and I. Kim. 2007. Test of larvicidal effect of some commercial natural products on lepidoptran Plutella xylostella and Spodoptera litura larvae. Korean J. Appl. Entomol. 15:87-91.
  17. Kim, H.H., Y.S. Seo, J.H. Lee, and K.Y. Cho. 1990. Development of fenvalerate resistance in the diamondback moth, Plutella xylostela Linne (Lepidoptera : Yponomeutidae) and its cross resistance. Korean J. Appl. Entomol. 29:194-200.
  18. Ko, H.S., R.D. Jin, B.H. Krishnan, S.B. Lee, and K.Y. Kim. 2009. Biocontrol Ability of Lysobacter antibioticus HS124 Against Phytophthora Blight Is Mediated by the Production of 4-Hydroxyphenylacetic Acid and Several Lytic Enzymes. CurrMicrobiol 59: 608-615.
  19. Lee, Y.S. 2010. Biological control of root-knot nematode (Meloidogyne incognita) in tomato by Lysobacter antibioticus HS124. A Master' dissertation, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea.
  20. Luo, W.C., S.D. Wang, S.J. Xu, and D. Qi. 2005. Inhibitory effects of 4-dodecylresorcinol on the phenoloxidase of the diamondback moth Plutella xylostella (L.) (Lepidoptera Plutellidae). Pestic. Biochem. Physiol. 82:52-58. https://doi.org/10.1016/j.pestbp.2004.12.005
  21. Russell, A.D. and J.R. Furr. 1996. Biocides; Mechanisms of antifungal action and fungal resistance. Sci. Prog. 79:27-48.
  22. Salinas, P.J. 1972. Studies on the ecology and behaviour of the larvae of Plutella xylostella L. (Lepidoptera: Plutellidae). Ph.D. Thesis Univ. of London. 356.
  23. Stern, V.M., R.F. Smith, R. Van den Bosch, and K.S. Hagen. 1959. The integrated control concept. Hilgardia. 29:81-101.
  24. Shoda, M. 2000. Bacterial control of plant diseases. J Biosci. Bioeng. 89:515-521. https://doi.org/10.1016/S1389-1723(00)80049-3
  25. Talekar, N.S. and A.M. Shelton. 1993. Biology, ecology and management of diamondback moth. Annu. Rev, Entomol. 38:275-301. https://doi.org/10.1146/annurev.en.38.010193.001423
  26. Taoka, Y., N. Naoki, O. Yuji, I. Hitoshi, S. Shinichi, and H. Masahiro. 2008. Effect of addition of Tween 80 and potassium dihydrogenphosphate to basal medium on the isolation of marine Eukaryotes, Thraustochytrids. J. Biosci. Bioeng. 105:562-565. https://doi.org/10.1263/jbb.105.562
  27. Yoon, C.S., T.J. Yoon, H.S. Park, S.G. Lee, J.K. Yoo, and J.O. Lee. 1998. Field evaluation of conidia of Beauveria bassiana (Baldamo) Vuillemin strain CS-1 against diamondback moth larvae. J. Pesti. Sci. 2:113-118.
  28. Wollum, A.G. 1982. Cultural methods for soil microorganism. Methods of Soil Analysis. Part2. Chemical and Microbiological Properties, American Society of Agronomy, Madison, Wisconsin, USA. 781-802.