Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
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Optimization of gibberellic acid production by Methylobacterium oryzae CBMB20

지베렐린산 생산을 위한 Methylobacterium oryzae CBMB20의 최적 배양조건 확립

  • Siddikee, Md. Ashaduzzaman (Dept. of Agricultural Chemistry, Chungbuk National University) ;
  • Hamayun, Muhammad (Dept. of Botany, Abdul Wali Khan University) ;
  • Han, Gwang-Hyun (Dept. of Agricultural Chemistry, Chungbuk National University) ;
  • Sa, Tong-min (Dept. of Agricultural Chemistry, Chungbuk National University)
  • ;
  • ;
  • 한광현 (충북대학교 농업생명환경대학 농화학과) ;
  • 사동민 (충북대학교 농업생명환경대학 농화학과)
  • Received : 2010.08.07
  • Accepted : 2010.08.13
  • Published : 2010.08.31
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Abstract

Gibberellic acid ($CA_3$) is used in many industries and constitutes the primary gibberellins produced by fungi and bacteria. However, there is no information on $CA_3$ production by Methylobacterium oryzae CBMB20, a novel plant growth promoting bacterium. We investigated the favorable carbon (C) and nitrogen (N) sources and ratios and cultural conditions, such as incubation temperature, pH of the culture medium, and incubation period for the maximum production of $CA_3$ by Methylobacterium oryzae CBMB20. Maximum $CA_3$ production was observed in ammonium mineral salt (AMS) broth supplemented with Na-succinate and $NH_4Cl$ as C and N sources, respectively. The maximum $CA_3$ production was found at the C/N ratio of 5:0.4 g $L^{-1}$. The highest $CA_3$ production was obtained when the bacterial culture was incubated at $30^{\circ}C$ for 96 h at pH 7.

지베렐린산은 곰팡이 및 세균으로부터 생산되는 주요 식물생장촉진물질이며 다양한 산업에서 이용되고 있다. 본 연구는 Methylobacterium oryzae CBMB20을 이용하여 GA3를 생산하기 위해 탄소원 및 질소원을 선발하였으며, 선발된 탄소원 및 질소원의 최적 농도와 최적 비율을 조사하였다. 탄소원으로는 Na-succinate가 methanol, glucose, maltose, sucrose, fructose, lactose에 비해 가장 우수하였으며, 질소원으로는 $NH_4Cl$가 $NO_3$, $NaNO_3$, glycine 등에 비해 우수하였다. 배양액에서 Nasuccinate와 $NH_4Cl$를 각각 5 및 0.4 g $L^{-1}$ 농도 비율로 사용하였을 때 $CA_3$의 생산량이 가장 높았다. 또한 ammonium mineral salt 배지의 pH를 7로 유지하고 $30^{\circ}C$의 조건으로 96시간 배양하였을 때 $CA_3$의 생산이 최대로 나타나는 것을 확인하였다.

Keywords

References

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