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A study on the development of high functional food protein ingredient from rice bran

고기능성 쌀단백질 소재 개발 연구

  • Lee, Eui-Suk (Dept. of Food Science and Technology, College of Agriculture and Life Science, Chungnam National University) ;
  • Kim, Ki-Jong (Division of barly and rice, National Institute Crops Science) ;
  • Kim, Jae-Hyeon (Division of Agriculture & Food resources, National Academy of Agriculture Science) ;
  • Hong, Soon-Taek (Dept. of Food Science and Technology, College of Agriculture and Life Science, Chungnam National University)
  • 이의석 (충남대학교 농업생명과학대학 식품공학과) ;
  • 김기종 (농촌진흥청 국립식량과학원 벼맥류부) ;
  • 김재현 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 홍순택 (충남대학교 농업생명과학대학 식품공학과)
  • Received : 2010.04.07
  • Accepted : 2010.06.11
  • Published : 2010.06.30

Abstract

Rice bran proteins from different cultivars(Youngan, Sindongjin, Suwon 511) were extracted with Xylanase using orthogonal analysis method and their functional properties were investigated. The optimum extraction conditions, based on protein content in the extract found to be at 1 wt% xylanase, pH 7 and 50:1, solvent to rice bran ratio(v/w %). Nitrogen solubility indices(NSI) of rice bran protein concentrates were shown a minimum value at pH 4 ranged 2~23%, varied with different cultivars and a maximum (NSI${\geq}$90% for all cultivars) at pH 10. As for water adsorption and fat adsorption capacity, rice bran protein concentrates were shown to be better than Na-caseinate and isolated soy protein, respectively. Emulsifying activities were observed high in order of Na-caseinate>Youngan rice bran protein>Shindongjin rice bran protein>Suwon 511 rice bran protein>isolated soy protein. In general, the surface tension of rice bran protein solution($10^{-3}$ wt%, 5 mM bis-tris, pH 7) was increased with increasing concentrations and found a minimum value near pI. On heating, it was decreased slightly with increasing temperatures up to $70^{\circ}C$ and then increased above $80^{\circ}C$. Addition of sodium chloride was made the surface tension decrease. In conclusion, with Xylanase, rice bran protein concentrate can be successfully extracted from the rice bran of different cultivars and the Youngan rice bran protein was thought to have best functionality among rice cultivars tested. It might be used as a milk protein substitute.

Acknowledgement

Supported by : 농촌진흥청

References

  1. Aoki, H., Taneyama, O., Orimo, N. and Kitagawa, I. 1981. Effect of lipophilization of soy protein on its emulsion stabilizing properties. J. Food Sci., 46:1192-1195. https://doi.org/10.1111/j.1365-2621.1981.tb03021.x
  2. Burks, A. W. and Helm, R. M. 1994. Hypoallergenicity of rice protein. Presented at the Annual Meeting of the American Association of Cereal Chemists, Nashville, TN.
  3. Bera, M. B. and Mukherjee, R. K. 1989. Solubility, emulsifying and foaming properties of rice bran protein concentrates. J. Food Sci., 54:142-145. https://doi.org/10.1111/j.1365-2621.1989.tb08587.x
  4. Hanmoungjai, P., Pyle, D. L. and Niranjan, K. 2002. Enzyme-assisted water-extraction of oil and protein from rice bran. J. Chemical Technology and Biotechnology, 77:771-776. https://doi.org/10.1002/jctb.635
  5. Hettiarachchy, N. S., Tang, S., Horex, R. and Eswaranandam, S. 2003. Physicochemical properties and functionality of rice bran protein hydrolyzate propared from heat-stabilized defatted rice bran with the aid of enzymes. J. Food Sci., 68(1): 152-157. https://doi.org/10.1111/j.1365-2621.2003.tb14132.x
  6. Hong, Soon Taek. 1996. Influence of surfactants on properties of $\beta$-Lactoglobulin emulsions and heat-treated emulsion gels. UK: Ph.D. thesis, University of Leeds.
  7. Jariwalla, R. J. 2001. Rice-bran products: Phytonutients with potential applications in preventive and clinical medicine. Drugs Experimental Clinical Research, 27:17-26.
  8. Kahlon, T. S., Chow, F. I. and Sayre, R. N. 1994. Cholesterol-lowering properties of rice bran. Cereal World, 39: 99-103.
  9. Kim, S. R., Ahn, J. Y., Lee, H. Y. and Ha, T. Y. 2004 Various properties and phenolic acid contents of rices and rice brans with different milling fractions. Korean J. Food Sci. Technol., 36:930-936.
  10. Kinsella, J. E. and Pearce, K. N. 1978. Emulsifying properties of proteins: Evaluation of a turbidimetric technique. J. Agric. Food Chem., 26(3):716-723. https://doi.org/10.1021/jf60217a041
  11. O'dell, B. L. and DeBoland, A. R. 1976. Complexation of phytate with proteins and cations in corn germ and oil seed meals. J. Agric. Food Chem., 24(4):804-808 https://doi.org/10.1021/jf60206a034
  12. Phillips, M. C. and Graham, D. E. 1979. Proteins at liquid interfaces : III. Molecular structures of adsorbed films. J. Colloid and Interface Sci., 70(3):427-439 https://doi.org/10.1016/0021-9797(79)90050-X
  13. Quinn, J. R. and Paton, D. 1979. A practical measurement of water hydration capacity of protein materials. Cereal Chem., 56:38-40.
  14. Shih, F, F., Champagne, E. T., Daigle, K. and Zarins, Z. 1999. Use of enzyme in the processing of protein products from rice bran an rice flour. Nahrung, 43(1):14-18. https://doi.org/10.1002/(SICI)1521-3803(19990101)43:1<14::AID-FOOD14>3.0.CO;2-K
  15. Shimizu, M., Saito, M. and Yamauchi, K. 1986. Hydrophobicity and emulsifying activity of milk proteins. Agric Biol Chem., 50:791-792. https://doi.org/10.1271/bbb1961.50.791
  16. Siebenmorgen, T., Wang, M., Hettiarachchy, N. S., Qi, M., and Burks, W. 1999. Preparation and Functional properties of rice bran protein isolate. J. Agric. Food Chem., 47:411-416. https://doi.org/10.1021/jf9806964
  17. Thompson, L. U., Liu, R. F. K. and Jones, J. D. 1982. Functional properties and food applications of rapeseed protein concentrate. J. Food Sci., 47:1175-118. https://doi.org/10.1111/j.1365-2621.1982.tb07643.x
  18. Tornberg, E. and Lundh, D. 1978. Functional characterization of protein stabilizated emulsions.: creaming stability. J. Food Sci., 43:1553-1558. https://doi.org/10.1111/j.1365-2621.1978.tb02541.x
  19. Yu, Kyung Jin, 2008. Emulsifying properties of rice bran protein. Korea: MS. thesis, Chung Nam National Univ.