Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
권호 표지
DOI QR코드

DOI QR Code

Effect of 1-MCP and High $pCO_2$ Treatment on the Firmness and Pectin Changes in Peach(Prunus persica) Fruit During Shelf-life

1-Methylcyclopropene(1-MCP) 및 $CO_2$ 처리가 복숭아(Prunus persica) 과실의 경도와 세포벽 변화에 미치는 영향

  • Kim, Myun-Surn (Dept. of Horticulture, College of Agriculture and Life Science, Chungnam National University) ;
  • Min, Jeong-Ho (Dept. of Horticulture, College of Agriculture and Life Science, Chungnam National University) ;
  • Chun, Jong-Pil (Dept. of Horticulture, College of Agriculture and Life Science, Chungnam National University) ;
  • Kim, Jin-Guk (Dept. of Fruit Science, National Institute of Horticultural & Herbal Science, RDA) ;
  • Lee, Eun-Mo (Dept. of Fruit Crops, Chungcheongnam Do, Agricultural Research and Extension Service) ;
  • Lee, Ji-Yong (Dept. of Fruit Crops, Chungcheongnam Do, Agricultural Research and Extension Service) ;
  • Hwang, Yong-Soo (Dept. of Horticulture, College of Agriculture and Life Science, Chungnam National University)
  • 김명선 (충남대학교 농업생명과학대학 원예학과) ;
  • 민정호 (충남대학교 농업생명과학대학 원예학과) ;
  • 천종필 (충남대학교 농업생명과학대학 원예학과) ;
  • 김진국 (국립원예특작과학원 과수과) ;
  • 이은모 (충남농업기술원 원예연구과) ;
  • 이지용 (충남농업기술원 원예연구과) ;
  • 황용수 (충남대학교 농업생명과학대학 원예학과)
  • Received : 2010.06.24
  • Accepted : 2010.09.17
  • Published : 2010.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

In order to understand the effects of a single or combined treatments of 1-MCP($1{\mu}L/L$) and $CO_2$(100%) on the firmness of melting type peach fruit(cv. Chunjungdo), fruit were harvested at commercial maturity and examined physiological changes including flesh firmness during 10 days of shelf-life. Firmness loss of fruit was delayed by both single and combined treatments of 1-MCP and $CO_2$. The treatment of 1-MCP was more effective than $CO_2$ treatment but no additive effective on firmness retention was found in the combined treatment. The upsurge of ethylene evolution occurred 5 days of shelf-life in air treated control but ethylene evolution gradually increased in fruit treated by 1-MCP and 1-MCP+$CO_2$. The suppression of ethylene evolution seemed stronger in $CO_2$ treatment. The respiration of fruit significantly inhibited up to 10 days except control where climacteric increase of respiration was found at 10 days of shelf-life. A molecular shift of pectic polymers(an increase of chelator soluble pectins and decrease of water soluble pectins) was induced by both 1-MCP and $CO_2$ treatments. An increase of water soluble pectins was coincident with firmness loss. The delay of firmness loss seemed to be associated with the migration of calcium to wall matrix, especially pectins, resulting in the increase of wall bound calcium. The polygalacturonase activity was significantly reduced by 1-MCP alone 1 day after treatment and increased to similar level of activity 5 days after treatment compared to other treatment except air treated control whereas pectin methylesterase activity seemed not to be affected by both 1-MCP and $CO_2$ treatments. Thus, the molecular shift of pectic polymers appeared not to be related with pectin methylesterase. Further study is required to clarify the softening mechanism associated with molecular shift of pectic polymers and the inter- or intra-cellular movement of calcium ions induced by postharvest treatments of 1-MCP and $CO_2$.

Keywords

References

  1. Blankenship, S.M., J.M. Dole. 2003. 1- Methylcyclopropene: a review. Postharvest Biology and Technology. 28: 1-25. https://doi.org/10.1016/S0925-5214(02)00246-6
  2. Bonghi, C., A. Ramina, B. Ruperti, R. Vidrih, P. Tonutti. 1999. Peach fruit ripening and quality in relation to picking time, and hypoxic and high $CO_{2}$ short-term postharvest treatments. Postharvest Biology and Technology. 16: 213-222. https://doi.org/10.1016/S0925-5214(99)00028-9
  3. Blumenkrantz, N., H.M. Asoe-Hansen. 1973. New method for quantitative determination of uronic acids. Anal. Biochem. 54: 484-489. https://doi.org/10.1016/0003-2697(73)90377-1
  4. Brummell, D.A. 2006. Cell wall disassembly in ripening fruit(Review). Functional Plant Biology. 33: 103-119. https://doi.org/10.1071/FP05234
  5. Byun, J.K., I.K. Kang, K.H. Chang, D.H. Kim, D.H. Lee, I.S. Kim, S.L. Shin, D.G. Choi. 2003. Changes of pectic substances and polygalacturonase activity during fruit development of various peach cultivars with degrees of fruit softening. J. Kor. Soc. Hort. Sci. 44: 503-507.
  6. Choi, S.J. 2005. Comparison of the change in quality and ethylene production between apple and peach fruits treated with 1-methylcyclopropene(1-MCP). Korean J. Food Preserv. 12: 511-515.
  7. Choi, J.H., J.H. Lim, M.C. Jeong, D.Kim. 2007. Effect of $CO_{2}$ treatment on postharvest quality of 'Kurakatawase' peach fruits. Kor. J. Hort. Sci. Technol. 25: 54-58.
  8. Choi, S.T., D.J. Huber. 2009. Differential sorption of 1-MCP to fruit and vegetable tissues, storage and cell wall polysaccharides, oils and lignins. Postharvest Biology and Technology. 52: 62-70. https://doi.org/10.1016/j.postharvbio.2008.11.002
  9. Cin, V.D., F.R. Massimo, A. Botton, P. Tonutti. 2006. The ethylene biosynthetic and signal transduction pathways are differently affected by 1-MCP in apple and peach fruit. Postharvest Biology Technology. 42: 125-133. https://doi.org/10.1016/j.postharvbio.2006.06.008
  10. De Wild, H.P.J., E.C. Otma, H.W. Peppelenbos. 2003. Carbon dioxide action on ethylene biosynthesis of preclimacteric and climacteric pear fruit. J. Expt. Bot. 54: 1537-1544. https://doi.org/10.1093/jxb/erg159
  11. De Wild, H.P.J., P.A. Balk, E.C.A. Fernandes, H.W. Peppelenbos. 2005. The action site of carbon dioxide in relation to inhibition of ethylene production in tomato fruit. Postharvest Biology and Technology 36: 273-280. https://doi.org/10.1016/j.postharvbio.2005.02.004
  12. Grant, G.T., Morris, E.R., Rees, D.A., Smith, P.C.J., Thom, D. 1973. Biological interactions between polysaccharides and divalent cations: the egg-box model. FEBS Lett. 32: 195-198. https://doi.org/10.1016/0014-5793(73)80770-7
  13. Gross, K.C. 1982. A rapid and sensitive spectrophotometric method for assaying polygalacturonase using 2- cyanoacetamide. HortScience 27: 933-934.
  14. Hagerman, A.E., P.J. Austin. 1986. Continuous spectrophotometric assay for plant pectin methyl esterase. J. Agric. Food Chem. 34: 440-444. https://doi.org/10.1021/jf00069a015
  15. Hayama, H., M. Tatsuki, A. Ito, Y. Kashimura. 2006. Ethylene and fruit softening in the stony hard mutation in peach. Postharvest Biology and Technology. 41: 16- 21. https://doi.org/10.1016/j.postharvbio.2006.03.006
  16. Huber, D.J. 2008. Suppression of ethylene responses through application of 1-methylcyclopropene: a powerful tool for elucidating ripening and senescence mechanisms in climacteric and nonclimacteric fruits and vegetables. HortScience 43: 106-111.
  17. Jeong, J., D.J. Huber, S.A. Sargent. 2002. influence of 1-methylcyclopropene(1-MCP) on ripening and cell-wall matrix ploysaccharides of avocado(Persea americana) fruit. Postharvest Biology and Technology. 25: 241-256. https://doi.org/10.1016/S0925-5214(01)00184-3
  18. Kluge, F.A., A.P. Jacomino. 2002. Shelf life of peaches treated with 1-Methylcyclopropene. Scientia Agricola. 59: 69-72. https://doi.org/10.1590/S0103-90162002000100010
  19. Karakurt, Y., D.J. Huber, W.B. Sherman. 2000. Qaulity characteristics of melting and non-melting flesh peach genotypes. J. Sci. Food Agric. 80: 1848-1853. https://doi.org/10.1002/1097-0010(200010)80:13<1848::AID-JSFA732>3.0.CO;2-0
  20. Lee, J.C., N.H. Kubota, Y.S. Hwang, J.P. Chun. 2007. Recent cultivation technoloy of peach fruit. pp. 175- 176. Seonjin Pub. Co., Ltd. Gyeonggi, Korea.
  21. Liguori, G., A. Weksler, Y. Zutahi, S. Lurie, I. Kosto. 2004. Effect of 1-methylcyclopropene on ripening of melting flesh peaches and nectarines. Postharvest Biology and Technology. 31: 263-268. https://doi.org/10.1016/j.postharvbio.2003.09.007
  22. Matsumoto, K., Y.S. Hwang, C.H. Lee, D.J. Huber. 2010. Changes in firmness and pectic polysaccharise solubility in three cultivars of strawberry fruit following short-term exposure to high p$CO_{2}$. Journal of Food Quality 33: 312-328. https://doi.org/10.1111/j.1745-4557.2010.00319.x
  23. Murayama, H., M. Arikawa, Y. Sasaki, V.D. Cin, W. Mitsuhashi, T. Toyomasu. 2009. Effect of ethylene treatment on expression of polyuronide-modifying genes and solubilization of polyuronides during ripening in two peach cultivars having different softening characteristics. Postharvest Biology and Technology. 52: 196-201. https://doi.org/10.1016/j.postharvbio.2008.11.003
  24. Seo, J.S. C.H. Han, M.S. Kim, C.H. Lee, B.G. Kim, J.H. Pak, Y.S. Hwang. 2007. Influence of ethylene removal or 1-MCP treatment on shelf-life in peach fruit. Kor. J. Hort. Sci. Technol. 25(Suppl. I): 183.
  25. Sisler, E.C., Serek, M., 1997. Inhibitors of ethylene responses in plants at the receptor level: recent developments. Physiol. Plant. 100: 577-582. https://doi.org/10.1111/j.1399-3054.1997.tb03063.x
  26. Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartener, F.H., Provenzano, M.D., Fujimoto, E.K., Goeke, N.M., Olson, B.J., Klenk, D.C. 1985. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150: 76-85. https://doi.org/10.1016/0003-2697(85)90442-7
  27. Smith, R.B., L.J. Skog. 1992. Postharvest carbon dioxide treatment enhances firmness of several cultivars of strawberry. HortScience 27: 420-421.
  28. Tibbits, C.W., A.J. Macdlugall, S.G. Ring. 1998. Calcium binding and swelling behaviour of a high methoxyl pectin gel. Carbohydr. Res. 310: 101-107. https://doi.org/10.1016/S0008-6215(98)00172-4
  29. Yang, H.S., G.P. Feng, H.J. An, Y.F. Li. 2006a. Microstructure changes of sodium carbonate-soluble pectin of peach by AFM during controlled atmosphere storage. Food Chemistry. 94: 179-192. https://doi.org/10.1016/j.foodchem.2004.11.003
  30. Yang, H., S. Lai, H An, Y. Li. 2006b. Atomic force microscopy study of the ultrastructural changes of chelatesoluble pectin in peaches under controlled atmosphere storage. Postharvest Biology and Technology. 39: 75-83. https://doi.org/10.1016/j.postharvbio.2005.08.001