Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Molecular Cloning and Characterization of cDNA Encoding Farnesyl Diphosphate Synthase in Garlic (Allium sativum)

마늘의 Farnesyl Diphosphate Synthase 유전자 클로닝 및 분자생물학적 특성규명

  • Pham, Anh Tuan (Department of Crop Science, Chungnam National University) ;
  • Park, Nam-Il (Department of Crop Science, Chungnam National University) ;
  • Kim, Haeng-Hoon (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Park, Sang-Un (Department of Crop Science, Chungnam National University)
  • ;
  • 박남일 (충남대학교 응용식물학과) ;
  • 김행훈 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 박상언 (충남대학교 응용식물학과)
  • Received : 2010.11.17
  • Accepted : 2010.12.13
  • Published : 2010.12.31
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Abstract

Farnesyl diphosphate synthase (FPS) catalyzes the biosynthesis of farnesyl diphosphate, a precursor for many important terpenoid products. A cDNA encoding FPS was first isolated from Allium sativum (AsFPS) using rapid amplification of cDNA ends (RACE) PCR. The sequence of AsFPS contains an open reading frame encoding a protein of 341 amino acids with a predicted molecular mass of 39.61 kDa. Alignment of AsFPS deduced amino acid revealed high identities with other plants ranging from 79% to 85% and showed 2-high conserved aspartate-rich motifs known to be important for FPS activity. Furthermore, AsFPS expression was stronger in the green organs such as bulbils, scapes, leaves, stems, but weaker in bulbs and roots than on-green organs of A. sativum.

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References

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