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Variation of Essential Oil Content and Its Composition during Callus Subculture of Peppermint (Mentha piperita)

페퍼민트 캘러스 계대배양 기간 중 정유함량과 성분변화

  • Park, Jung-Suk (Department of Complementary & Alternative Medicine, Kwangju Women's University) ;
  • Park, Woo-Tae (Department of Crop Science, Chungnam National University) ;
  • Kim, Haeng-Hoon (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Park, Sang-Un (Department of Crop Science, Chungnam National University)
  • 박정숙 (광주여자대학교 대체의학과) ;
  • 박우태 (충남대학교 응용식물학과) ;
  • 김행훈 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 박상언 (충남대학교 응용식물학과)
  • Received : 2010.10.13
  • Accepted : 2010.12.13
  • Published : 2010.12.31

Abstract

Peppermint (Mentha piperita L.) belongs to a member of the mint family (Lamiaceae) and is widely used in food, cosmetics and medicines. This study was carried to investigate the variation of essential oil content and its composition during callus subculture of M. piperita. For callus induction from the leaf explant of peppermint, the basal medium was supplemented with various concentrations of 2, 4-D. The best callus induction rate (93%) of M. piperita. was obtained in MS medium containing 2 mg/l 2, 4-D. The induced peppermint callus maintained on Lin-Staba medium were studied during a period of 20th subcultures for the stability of essential oil production. Growth rates of peppermint callus increased during prolonged subculture. However, there was a progressive decrease of essential oil content and unstability of monoterpene productions when callus cultures were serially subcultured.

Keywords

References

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