Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Isolation and Characterization of the Auxin producing Plant Growth Promoting Rhizobacterium from Soil in a Ginseng Field

인삼토양으로부터 옥신 생성 식물생장촉진세균의 분리 및 특성

  • Bak, Hae-Sung (Dept. of Bio-Environmental Chemistry, Chungnam National University) ;
  • Jung, Young-Pil (Dept. of Bio-Environmental Chemistry, Chungnam National University) ;
  • Yoon, Min-Ho (Dept. of Bio-Environmental Chemistry, Chungnam National University)
  • 박해성 (충남대학교 생물환경화학과) ;
  • 정영필 (충남대학교 생물환경화학과) ;
  • 윤민호 (충남대학교 생물환경화학과)
  • Received : 2010.10.21
  • Accepted : 2010.12.13
  • Published : 2010.12.31
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Abstract

An auxin-producing bacterium (AMT-54) was isolated from ginseng cultivating soil of Geumsan area. The isolate AMT-54 was confirmed to produce indole-3-acetic acid (IAA) which is one of auxin hormone by TLC analysis. When the concentration of IAA was assessed by performing HPLC quantitative analysis, the maximal 457ppm of IAA was detected from the culture filtrate after culturing in R2A broth containing 0.1% tryptophan for 24h at $35^{\circ}C$. The molecular weight of the main peak obtained by LC-mass analysis was correspondent well to 175, that of IAA. The strain AMT-54 was identified as a novel species belongs to Klebsiella mobilis by a chemotaxanomic and phylogenetic analysis. To investigate the growth promoting effect of crop, when the culture broth of K. mobilis AMT-54 was infected onto seed pot of mung bean, the adventitious root induction and root growth of mung bean were 3.3times higher than control.

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References

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