Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Studies of Exercise-Induced Allergy Anaphylaxis Mechanisms and the Effects of Vitamin C and Catalase Supplementation in Exercise-Induced Allergy Anaphylaxis Models

운동 유발성 알레르기 질환분석 및 비타민 C와 catalase 투여 효과 분석

  • Kwak, Yi-Sub (Laboratory of Measurement and evaluation for exercise physiology & physical fitness, Department of Physical Education, Dong-Eui University)
  • 곽이섭 (동의대학교 체육학과, 측정평가 및 운동생리학 실험실)
  • Received : 2009.11.03
  • Accepted : 2010.03.25
  • Published : 2010.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

Exercise-induced anaphylaxis (EIA) is defined as the onset of allergic symptoms during, or immediately after, exercise, the clinical signs being various degrees of urticaria, angioedema, respiratory and gastrointestinal signs, and even anaphylactic shock. Food-dependent exercise-induced anaphylaxis (FDEIA) is a specific variant of exercise-induced anaphylaxis that requires both vigorous physical activity and the ingestion of specific foods within the preceding several hours. To describe the physiopathologic mechanism, etiologic factors, and clinical manifestations, we evaluated the supplementation of vitamin C and catalase on spleen index, proliferation assay, ROS, and ASAS in sensitized and exercise trained mice. The results were as follows: Spleen index showed the highest level in the ST12 group compared to other groups; this level increased in a time dependent manner and in significant amounts. In proliferation assay of Med and OVA, the ST12 group showed the highest level compared to other groups; this level also increased in a time dependent manner. On the other hand, spleen ROS did not show a statistically significant difference, and peritoneal ROS showed the highest level in the ST12 group. ASAS showed the highest level in the ST12 compared to the S; this was also in a time dependent manner and in significant amounts. From the results, we chose the ST9 and ST12 groups to evaluate allergy anaphylaxis with supplementation of Vitamin C and catalase. In both the ST9 and ST12 groups, peritoneal ROS and ASAS were lower in vitamin C treatment group than in the catalase treatment group. This was a statistically significant difference. From the results, allergy anaphylaxis showed a higher level in the long trained group than in the short trained group. Also, treatment with vitamin C was more effective in lowering allergy anaphylaxis than catalase treatment.

전 세계적으로 알레르기 환자가 증가하고 있고, 특히 운동이 알레르기 질환을 증가 시켜 운동 유발성 알레르기에 대한 연구가 주목되고 있다. 운동 유발성 알레르기 질환은 운동 중과 운동 후 혈관부종, 두드레기, 호흡, 위와 장계의 증후 및 아나플락시스 등이 나타나는 질환이다. 따라서 본 연구는 운동 유발성 알레르기 질환의 분석 및 비타민 C와 catalase 투여에 대한 항 알레르기 효과와 활성 산소종 생성 조절제 들의 상호작용을 규명하고 전반적인 운동 유발성 알레르기 질환의 치료효과를 규명하는데 그 목적이 있다. 연구방법으로는 무병원성환경에서 사육한 생후 7주령의 BALE/c마우스(Female)를 그룹핑 하여 통제군과 훈련군에게 각각 알레르기를 유발한 후, 서로 다른 기간의 운동 효과를 분석하였고, 운동 유발성 알레르기 군에게 비타민c와 catalase를 투여하여 투여 효과를 분석하였다. 본 연구결과 운동 유발성 알레르기 질환은 훈련기간이 늘어남에 따라 증가함을 알 수 있었고, 이러한 운동 유발성 알레르기 질환과 쇼크사는 복강 림프구에서 발현하는 ROS는 큰 연관이 있다는 것, 그리고 운동 유발성 알레르기 치료 효과는 catalase를 투여하는 것 보다 비타민을 투여하는 것이 더욱 효과적임을 알 수 있었다. 추후 이에 대한 원인을 규명하는 기전적 연구가 필요할 것으로 여겨진다.

Keywords

References

  1. Al Suleimani, Y. M., M. J. Walker. 2007. Allergy rhinitis and its pharmacology. Pharmacol. Ther. 114, 233-260. https://doi.org/10.1016/j.pharmthera.2007.01.012
  2. Anderson, S. D. 2006. How does exercise cause asthma attacks? Curr. Opin. Allergy Clin. Immunol. 6, 37-42. https://doi.org/10.1097/01.all.0000199797.02423.78
  3. Barnes, P. J. 2006. Drugs for asthma. Br. J. Pharmacol. 147, 297-303. https://doi.org/10.1038/sj.bjp.0706437
  4. Chandra, R. K., M. Baker, S. Whang, and B. Au. 1991. Effect of two feeding formulas on immune responses and mortality in mice challenged with Listeria monocytogenes. Immunol. Lett. 27, 45-8. https://doi.org/10.1016/0165-2478(91)90242-3
  5. Espersen, G. T., A. Elbaek, S. Schmidt-Olsen, E. Ejlersen, K. Varming, and N. Grunnet. 1996. Short-term changes in the immune system of elite swimmers under competition conditions. Different immunomodulation induced by various type of sport. Scand. J. Med. Sci. Sports 6, 156-63.
  6. Holzer, K., S. D. Anderson, and J. Douglass. 2002. Exercise in elite summer athletes: challenges for diagnosis. J. Allergy Clin. Immunol. 110, 374-380. https://doi.org/10.1067/mai.2002.127784
  7. Katelaris, C. H., F. M. Carrozzi, T. V. Burke, and K. Byth. A. 2000. Springtime olympics demands special consideration for allergic athletes. J. Allergy Clin. Immunol. 106, 260-266. https://doi.org/10.1067/mai.2000.108603
  8. Kim, C. H. and Y. S. Kwak. 2004. Swim training increases ovalbumin induced active systemic anaphylaxis in mice. Immunol. Invest. 33, 469-80. https://doi.org/10.1081/IMM-200039190
  9. Komarow, H. D. and T. T. Postolache. 2005. Seasonal allergy and seasonal decrements in athletic performance. Clin. Sports Med. 24, 35-50. https://doi.org/10.1016/j.csm.2004.12.006
  10. Kruisbeek, A. M. 1998. In vitro assays for lymphocyte function. In Coligan, J. E., Am M. Kruisbeek, D. H. Margulies, E. M. Shevach, and W. Strober (eds.), Current protocols in immunology. vol.: 3.1.1 - 3.1.5, John Wiley and Sons, Inc., NY, USA.
  11. Kwak, Y. S. 2006. Effect of training on spleen and peritoneal exudate reactive oxygen species and lymphocyte proliferation by splenocytes at rest and after an acute bout of exercise. Sports Sci. 24, 973-8. https://doi.org/10.1080/02640410500386233
  12. Okayama, Y. 2005. Oxidative stress in allergy and inflammatory skin diseases. Curr. Drug Targets Inflamm. Allergy 4, 517-519. https://doi.org/10.2174/1568010054526386
  13. O'Sullivan, S., A. Roquet, B. Dahlen, F. Larsen, A. Eklund, M. Kumlin, P. M. O'Byrne, and S. E. Dahlen. 1998. Evidence for mast cell activation during exercise-induced bronchoconstriction. Eur. Respir. 12, 345-350. https://doi.org/10.1183/09031936.98.12020345
  14. Randolph, C. C., D. Dreyfus, K. W. Rundell, D. Bangladore, and B, Fraser. 2006. Prevalence of allergy and asthma synptoms in recreational road runners. Med. Sci. Sports Exerc. 38, 2053-2057. https://doi.org/10.1249/01.mss.0000235357.31932.43
  15. Schweitzer, C., L. T. Vu, Y. T. Nguyen, C. Chone, B. Demoulin, and F. Marchal. 2006. Estimation of the bronchodilatory effect of deep inhalation after a free run in children. Eur. Respir. J. 28, 89-95. https://doi.org/10.1183/09031936.06.00114705
  16. Scomparin, D. X., S. Grassiolli, A. C. Marcal, C. Gravena, A. E. Andreazzi, and P. C. Mathias. 2006. Swim training applied at early age is critical to adrenal medulla catecholamine content and to attenuate monosodium L-glutamate-obesity onset in mice. Life Sci. 79, 2151-2156. https://doi.org/10.1016/j.lfs.2006.07.012
  17. Skoner, D. P. 2001. Allergy rhinitis: definition, epidemiology, pathophysiology, detection, and diagnosis. J. Allergy Clin. Immunol. 108, S2-8. https://doi.org/10.1067/mai.2001.115569
  18. Suzuki, K., S. Nakaji, S. Kurakake, M. Totsuka, K. Sato, T. Kuriyama, H. Fujimoto, K. Shibusawa, K. Machida, and K. Sugawara. 2003. Exhaustive exercise and type-1/type-2 cytokine balance with special focus on interleukin-12 p40/p70. Exerc. Immunol. Rev. 9, 48-57.
  19. Suzuki, K., S. Nakaji, M. Yamada, M. Totsuka, K. Sato, and K. Sugawara. 2002. Systemic inflammatory response to exhaustive exercise. Cytokine kinetics. Exerc. Immunol. Rev. 8, 46-48.
  20. Suzuki, Y., T. Yoshimaru, T. Inoue, O. Niide, and C. Ra. 2005. Role of oxidants in mast cell activation. Chem. Immunol. Allergy 87, 32-42.
  21. Tsukahara, H. 2007. Biomarkers for oxidative stress: clinical application in pediatric medicine. Curr. Med. Chem. 14, 339-351. https://doi.org/10.2174/092986707779941177
  22. Von Garnier, C., M. Astori, A. Kettner, N. Dufour, G. Corradin, and F. Spertini. 2002. In vivi knetics of immunoglobulin E response to allergen: bystander effect of coimmunization and relationship with anaphylaxis. Clin. Exp. Allergy 32, 401-410. https://doi.org/10.1046/j.1365-2222.2002.01304.x
  23. Walls, R. S., R. J. Heddle, M. L. Tang, B. J. Basger, G. O. Solley, and G. T. Yeo. 2005. Optimising the management of allergic rhinitis: an Australian perspective. Med. J. Aust. 182, 28-33.
  24. Weiler, J. M., T. Layton, and M. Hunt. 1998. Asthma in United states Olympic athletes who participated in the 1996 Summer Games. J. Allergy Clin. Immunol. 102, 722-726. https://doi.org/10.1016/S0091-6749(98)70010-7
  25. Wiedermann, U., and A. Mercenier. 2007. New allergy intervention strategies: hitting the mucosal road. Clin. Exp. Allergy 37, 473-475. https://doi.org/10.1111/j.1365-2222.2007.02709.x

Cited by

  1. Effects of Different Physical Frequency on Food-Dependent Exercise Induced Allergy Anaphylaxis (FDEIA) and Related Mechanisms vol.22, pp.7, 2012, https://doi.org/10.5352/JLS.2012.22.7.897
  2. Combined effects of food and exercise on anaphylaxis vol.7, pp.5, 2013, https://doi.org/10.4162/nrp.2013.7.5.347