Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지

Effects of Lycii fructus and Astragalus membranaceus Mixed Extracts on Immunomodulators and Prevention of Diabetic Cataract and Retinopathy in Streptozotocin-induced Diabetes Rat Model

Streptozotocin으로 유발한 당뇨병성 쥐에서의 당뇨병성 백내장과 망막병증에 대한 구기자와 황기 혼합 추출물 등의 면역 조절 및 예방 효과

  • Jeon, Yun-Hui (College of Biomedical & Health Science, Department of Life Science, Konkuk University) ;
  • Moon, Jun-Woong (Department of ophthalmology, Konkuk University Medical Center, Konkuk University School of Medicine) ;
  • Kweon, Hyuk-Jung (Department of Family medicine, Konkuk University Chungju Hospital) ;
  • Jeoung, Young-Jun (College of Biomedical & Health Science, Department of Life Science, Konkuk University) ;
  • An, Chi-Sun (College of Biomedical & Health Science, Department of Life Science, Konkuk University) ;
  • Jin, Hai-Lan (College of Biomedical & Health Science, Department of Life Science, Konkuk University) ;
  • Hur, Sun-Jin (College of Biomedical & Health Science, Department of Life Science, Konkuk University) ;
  • Lim, Beong-Ou (College of Biomedical & Health Science, Department of Life Science, Konkuk University)
  • 전윤희 (건국대학교 의료생명대학 생명과학부) ;
  • 문준웅 (건국대학교 의학전문대학원 안과학 교실) ;
  • 권혁중 (건국대학교 의학전문대학원 가정의학과) ;
  • 정용준 (건국대학교 의료생명대학 생명과학부) ;
  • 안치선 (건국대학교 의료생명대학 생명과학부) ;
  • 김해란 (건국대학교 의료생명대학 생명과학부) ;
  • 허선진 (건국대학교 의료생명대학 생명과학부) ;
  • 임병우 (건국대학교 의료생명대학 생명과학부)
  • Received : 2009.08.28
  • Accepted : 2010.01.09
  • Published : 2010.02.28
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Abstract

This study was conducted to investigate the effects of Lycii fructus and Astragalus membranaceus mixed extracts on immunomodulators and prevention in a streptozotocin-induced diabetes rat model. A total of 28 male rats were divided into four dietary groups and fed a commercial diet (A), commercial diet plus induced diabetes by a streptozotocin (STZ) injection (B), induced diabetes by STZ plus medicinal crop extracts(I&$H^{(R)}$) diet (C), and medicinal crop extracts (I&$H^{(R)}$) diet (D). Immunoblotting analyses revealed cytokine expression, and ELISA analyses revealed immunoglobulin E and nitric oxide production. As a results, the tumor necrosis factor-$\alpha$ (TNF-$\alpha$) and inducible nitric oxide synthase (iNOS) as a inflammatory cytokine were decreased. Interleukin-6 (IL-6) and signal transducer and activation of transcription 3 (STAT3) cytokine related in diabetes expression through JAK/STAT3 pathway were also decreased. Furthermore, immunoglobulin E and nitric oxide production were decreased in the serum and lens, respectively. These results suggest that Lycii fructus and Astragalus membranaceus mixed extracts provide positive effects on immunomodulators and prevention in diabetes and eye disease complications.

Keywords

References

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