The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Synergistic Effect of Notopterygium incisum with Clematis manshurica in the Anti-inflammatory Activity

강활(羌活)과 위령선(威靈仙)의 항염증 상승작용에 관한 연구

  • Kim, Seung-Ju (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Chun, Jin-Mi (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Yang, Won-Kyung (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Cheon, Myeong-Sook (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Sung, Yoon-Young (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Park, Jun-Yeon (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Kim, Ho-Kyoung (Center of Herbal Resources Research, Korea Institute of Oriental Medicine)
  • 김승주 (한국한의학연구원 한약자원연구센터) ;
  • 천진미 (한국한의학연구원 한약자원연구센터) ;
  • 양원경 (한국한의학연구원 한약자원연구센터) ;
  • 전명숙 (한국한의학연구원 한약자원연구센터) ;
  • 성윤영 (한국한의학연구원 한약자원연구센터) ;
  • 박준언 (한국한의학연구원 한약자원연구센터) ;
  • 김호경 (한국한의학연구원 한약자원연구센터)
  • Received : 2010.11.08
  • Accepted : 2010.12.15
  • Published : 2010.12.30
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Abstract

Objective : Oriental medicines have been combined oriental medical theory which based on the seven modes of emotions. Notopterygium incisum (N. incisum) and Clematis manshurica (C. manshurica) have been used as an anti-rheumatic and analgesic medicine for the treatment of rheumatism, headache, cold, etc. In this study, we evaluate the synergistic anti-inflammatory effect of N. incisum and C. manshurica. Method : To evaluate the synergistic anti-inflammatory effect of a herbal mixture N. incisum and C. manshurica, we examined the changed ear thickness in 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-induced mouse ear edema model after topical application of herbal mixture. In addition, the levels of markers for inflammation, such as tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-$1{\beta}$, prostaglandin $G_2$ ($PGE_2$), and nitric oxide (NO) were measured by ELISA assay and Griess reagent in lipopolysaccharide-stimulated Raw 264.7 cells. Results : Our results showed that aqueous extracts of N. incisum and C. manshurica combination significantly inhibited the mouse ear edema induced by TPA. Moreover, the aqueous extracts of N. incisum and C. manshurica combination exhibited synergistic effects in down-regulating TNF-${\alpha}$, IL-$1{\beta}$, $PGE_2$ level, but not NO. Conclusions : This study suggested that combined treatment of N. incisum and C. manshurica, based on seven methods in prescription compatibility, has a synergistic effect in down-regulating inflammatory response both in vitro and in vivo models.

Keywords

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