The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Neuroprotective Effects of Agrimoniae Herba against Intrastriatal Hemorrhage in Rats

선학초(仙鶴草)가 선조체내출혈(線條體內出血) 흰쥐의 뇌조직 손상에 미치는 영향

  • Choi, Young-Seuk (Department of Anatomy-Pointlogy College of Oriental Medicine, Kyungwon University) ;
  • Kim, Youn-Sub (Department of Anatomy-Pointlogy College of Oriental Medicine, Kyungwon University)
  • 최용석 (경원대학교 한의과대학 해부경혈학교실) ;
  • 김연섭 (경원대학교 한의과대학 해부경혈학교실)
  • Received : 2010.11.15
  • Accepted : 2010.12.15
  • Published : 2010.12.30
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Abstract

Objects : This study was performed in order to evaluate the effects of Agrimoniae herba (AH) ethanol extract on intrastriatal hemorrhage (ISH). Method : ISH was induced by the stereotaxic intrastriatal injection of bacterial collagenase type IV in Sprague-Dawley rats. AH was orally given once a day for 3 days after ISH. Hematoma volume and percentage edema were examined. Immunohistochemistry was processed for iNOS, c-Fos, MMP-9, and MMP-12 expressions in the brain sections and each immuno-labeling were calculated with image analysis. Results : results are as follows; 1. AH reduced the hematoma volume and percentage edema of the ISH-induced rat brain. 2. AH swollen apoptotic bodies and neurons in the peri-hematoma regions of the ISH-induced rat brain. 3. AH significantly reduced c-Fos, MMP-9 and MMP-12 positive cells in the peri-hematoma regions of the ISH-induced rat brain. 4. AH swollen iNOS expressions in the peri-hematoma regions of the ISH-induced rat brain. Conclusion : These results suggest that AH plays an anti-apoptotic neuroprotective effect through control of ISH, suppression of c-Fos, and down-regulation of MMP-9 and MMP-12 expressions in the brain tissues.

Keywords

References

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