대한의생명과학회지 (Biomedical Science Letters)

  • 제16권1호
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  • Pages.46-52
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  • 2010
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  • 1738-3226(pISSN)
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  • 2288-7415(eISSN)
대한의생명과학회 (The Korean Society for Biomedical Laboratory Sciences)
권호 표지

Effects of Sphingosine-1-phosphate on Vestibular Nuclear Neurons

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  • 박종성 (전남대학교 의과대학 생리학교실)
  • Lee, Jae-Hyuk (Department of Pathology, Chonnam National University Medical School) ;
  • Jang, Su-Jeong (Department of Physiology, Chonnam National University Medical School) ;
  • Kim, Song-Hee (Department of Physiology, Chonnam National University Medical School) ;
  • Jeong, Han-Seong (Department of Physiology, Chonnam National University Medical School) ;
  • Park, Jong-Seong (Department of Physiology, Chonnam National University Medical School)
  • 투고 : 2009.09.14
  • 심사 : 2010.03.25
  • 발행 : 2010.03.31
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초록

This study was designed to investigate the effects of sphingosine-1-phosphate on the neuronal activity of rat medial vestibular nuclear neurons. Sprague-Dawley rats aged 14 to 16 days were decapitated under ether anesthesia. After treatment with pronase and thermolysin, the dissociated medial vestibular nuclear neurons were transferred into a chamber on an inverted microscope. Spontaneous action potentials and potassium currents were recorded by standard patch-clamp techniques under current and voltage-clamp modes respectively. 15 medial vestibular nuclear neurons revealed excitatory responses to 1 and $5\;{\mu}M$ of sphingosine-1-phosphate. The spike frequency and resting membrane potential of these cells were increased by sphingosine-1-phosphate. The amplitude of afterhyperpolarization was decreased by sphingosine-1-phosphate. Whole potassium currents of medial vestibular nuclear neurons were decreased by sphingosine-1-phosphate (n=12). Sphingosine-1-phosphate did not affect the charybdotoxin-treated potassium currents. These experimental results suggest that sphingosine-1-phosphate increases the neuronal activity of the medial vestibular nuclear neurons by altering the resting membrane potential and afterhyperpolarization.

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