Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Inhibitory Effect on Angiotensin-converting Enzyme (ACE) and Optimization for Production of Ovotransferrin Hydrolysates

Ovotransferrin 가수분해물의 Angiotensin-converting Enzyme 활성억제 효과 및 생산 최적화

  • Lee, Na-Kyoung (Division of Animal Life Science and Bio/Molecular Informatics Center, Konkuk University) ;
  • Ahn, Dong-Uk (Department of Animal Science, Iowa State University and Major in Biomodulation, Seoul National University) ;
  • Park, Keun-Kyu (Animal Resources Research Center, Konkuk University) ;
  • Paik, Hyun-Dong (Division of Animal Life Science and Bio/Molecular Informatics Center, Konkuk University)
  • 이나경 (건국대학교 동물생명과학부 및 생명분자정보학센터) ;
  • 안동욱 (아이오와주립대학교 동물과학과 및 서울대학교 Biomodulation) ;
  • 박근규 (건국대학교 동물자원연구센터) ;
  • 백현동 (건국대학교 동물생명과학부 및 생명분자정보학센터)
  • Received : 2009.12.17
  • Accepted : 2010.03.23
  • Published : 2010.04.30
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Abstract

Angiotensin-converting enzyme (ACE) inhibitory activity and production optimization of ovotransferrin hydrolysates were studied. Ovotransferrin was hydrolyzed by several enzymes (protamex, alcalase, trypsin, pepsin, neutrase, and flavorzyme) and acid (0.03 N HCl). Ovotransferrin hydrolysate reduced ACE activity by 60.2%, 55.8%, and 42.6% when treated with trypsin, acid, and pepsin, respectively. Trypsin was selected for production of peptide having maximum AC inhibitory effect, which was greatest with 7 h hydrolysis. Central composite design determined that optimum composition of ACE inhibitory substances using substrate concentration of 20-35%, temperature of $35-55^{\circ}C$, and pH of 6.0-8.0. The optimum composition was 1% trypsin, substrate concentration of 26.32%, $51.29^{\circ}C$, and pH 6.32. Under this conditions, a maximum ACE inhibitory effect of 69.1% was evident, similar to the predicted value.

본 연구를 통해 난백 단백질인 ovotransferrin 및 가수분해물의 항고혈압 효과를 얻을 수 있는 ACE 저해효과가 최초로 확인되었다. Ovotransferrin과 산 분해 및 효소 가수분해물의 ACE 저해효과 순서는 trypsin>pepsin>산 분해순인 것으로 나타났다. 따라서, trypsin에 의해 가수분해되어 생산된 가수분해물이 가장 큰 ACE 저해효과(60.2%)를 나타내는 것으로 확인되었으며, 이 결과를 토대로 trypsin에 의한 가수분해물의 최적화 연구를 수행하였다. ACE 저해효과를 효소 반응시간에 따른 생산을 검토한 결과, 7시간 일 때 64%로 가장 높게 나타났으며, 7시간을 반응시간으로 최적화 실험을 계속 진행하였다. 기질과 효소의 비, pH, 반응온도를 변수로 사용하여 중심합성계획에 의해 총 16개의 조건으로 하여 최적화 하였다. 회귀식의 전체 $R^2$는 0.9908이었고, 0.01% 유의수준을 나타내었다. 중심합성계획에 따른 maximum response는 기질 농도 26.32%, pH 6.32, $51.29^{\circ}C$에서 ACE 저해효과 70.67%로 예측되어졌으며, 실제 실험으로 검증한 결과도 이와 유사하게 69.1%로 확인됨으로써, 본 실험을 통해 ACE 저해활성을 나타내는 가수분해물의 생산이 최적화되었다.

Keywords

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