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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Comparative analysis of fat and muscle proteins in fenofibratefed type II diabetic OLETF rats: the fenofibrate-dependent expression of PEBP or C11orf59 protein

  • Hahm, Jong-Ryeal (Department of Internal Medicine, Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • Ahn, Jin-Sook (Department of Biochemistry, Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • Noh, Hae-Sook (Department of Biochemistry, Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • Baek, Seon-Mi (Department of Biochemistry, Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • Ha, Ji-Hye (Department of Biochemistry, Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • Jung, Tae-Sik (Department of Internal Medicine, Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • An, Yong-Jun (Department of Internal Medicine, Dong-A University College of Medicine) ;
  • Kim, Duk-Kyu (Department of Internal Medicine, Dong-A University College of Medicine) ;
  • Kim, Deok-Ryong (Department of Biochemistry, Institute of Health Sciences, Gyeongsang National University School of Medicine)
  • Received : 2010.01.15
  • Accepted : 2010.03.05
  • Published : 2010.05.31
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Abstract

Fenofibrate, an agonist of $PPAR{\alpha}$, plays an important role in activating many proteins catalyzing lipid metabolism, and it also has a considerable effect on improvement of insulin sensitivity in the diabetic condition. To investigate fenofibrate-dependent expression of peripheral tissue proteins in diabetes, we analyzed whole muscle or fat proteins of fenofibrate-fed OLETF rats, an animal model of type II diabetes, using 2-dimensional gel electrophoresis. We found that many proteins were specifically expressed in a fenofibrate-dependent manner in these diabetic rats. In particular, a functionally unknown C11orf59 protein was differentially expressed in the muscle tissues (about 5-fold increase) in fenofibrate-fed OLETF rats as compared to control rats. Additionally, the signal proteins phosphatidylethanolamine binding protein and IkB interacting protein were differentially regulated in the fenofibrate-treated adipose tissues. We suggest here that these proteins might be involved in controlling lipid or carbohydrate metabolism in diabetes via $PPAR{\alpha}$ activation.

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References

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