Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Biological Activity of Phenol Compound from a Cactus Cheonnyuncho (Opuntia humifusa) in Korea

천년초선인장으로부터 분리한 페놀성 화합물의 생리활성 효과

  • 이경석 (호서대학교 식품생물공학과) ;
  • 이기영 (호서대학교 식품생물공학과)
  • Received : 2010.05.06
  • Accepted : 2010.06.22
  • Published : 2010.08.31
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Abstract

After Cheonnyuncho (Opuntia humifusa) was extracted by 70% ethanol and partitioned with hexane, chloroform, ethyl acetate, butanol, and water in order, ethyl acetate fraction with excellent antioxidant activities was acquired. Ethyl acetate fraction was conducted by TLC, and the third spot of 10 spots was selected due to its best antioxidant activities. When the third fraction further isolated on silica gel chromatography, the fourth fraction of 10 fractions had the best antioxidant activities and purified by HPLC. The molecular weight on EI-MS, and $^1H$- and $^{13}C$-NMR spectrum showed that the purified compound was taxifolin. In addition, antioxidant activities were analyzed, and the purified compound from Cheonnyuncho was found to be effective as much as $\alpha$-tocopherol, BHA. According to the analysis on antibacterial activities, the purified compound also showed more activity than benzoic acid against all pathogenic bacteria.

천년초는 한국에서 자생하는 손바닥선인장으로 예전부터 민간에서 약재로 사용되어져 왔다. 천년초를 70% 에탄올로 추출한 후 이를 헥산, 클로로포름, 에틸아세테이트, 부탄올, 물 층으로 순차 분획하여 항산화 활성이 우수한 에틸아세테이트 획분을 얻었다. 에틸아세테이트 획분을 가지고 TLC를 실시하여 10개의 fraction으로 분리하였고 이중 항산화 활성이 가장 우수하게 나온 3번째 fraction을 취한 후 silica gel chromatography를 사용하여 정제하였다. 총 10개의 분획을 채취한 후 항산화 활성이 우수한 4번째 분획을 채취하여 HPLC를 사용하여 정제하였다. 정제물은 EI-MS를 사용하여 분자량을 측정하였고 $^1H$-NMR과 $^{13}C$-NMR spectrum을 측정하여 검증한 결과 taxifolin으로 확인되었다. 이를 가지고 항산화 활성을 검증한 결과 $\alpha$-tocopherol, BHA와 비교하여 뒤지지 않는 효과를 보여주었고 항균 활성을 검증한 결과 그람 양성, 음성의 식중독균에 모두 benzoic acid보다 우수한 활성을 보여주었다.

Keywords

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