Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Isolation and Identification of Antioxidative Compounds in Fermented Glasswort (Salicornia herbacea L.) Juice

함초발효액으로부터 항산화 활성 물질의 분리 및 동정

  • Cho, Jeong-Yong (Dept. of Food Engineering and Solar Salt Biotechnology Research Center, Mokpo National University) ;
  • Park, Sun-Young (Dept. of Food Engineering and Solar Salt Biotechnology Research Center, Mokpo National University) ;
  • Shin, Mi-Jeong (Dept. of Food Engineering and Solar Salt Biotechnology Research Center, Mokpo National University) ;
  • Gao, Tian-Cheng (Dept. of Food Engineering and Solar Salt Biotechnology Research Center, Mokpo National University) ;
  • Moon, Jae-Hak (Dept. of Food Science & Technology, and Functional Food Research Center, Chonnam National University) ;
  • Ham, Kyung-Sik (Dept. of Food Engineering and Solar Salt Biotechnology Research Center, Mokpo National University)
  • 조정용 (목포대학교 식품공학과 및 천일염생명과학연구소) ;
  • 박선영 (목포대학교 식품공학과 및 천일염생명과학연구소) ;
  • 신미정 (목포대학교 식품공학과 및 천일염생명과학연구소) ;
  • 고천성 (목포대학교 식품공학과 및 천일염생명과학연구소) ;
  • 문제학 (전남대학교 식품공학과 및 기능성식품연구센터) ;
  • 함경식 (목포대학교 식품공학과 및 천일염생명과학연구소)
  • Received : 2010.04.29
  • Accepted : 2010.06.09
  • Published : 2010.08.31
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Abstract

The chloroform layer of fermented glasswort (Salicornia herbacea) juice was found to have higher radical-scavenging activity than the other layers by the assays using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) ($ABTS^+$) radicals. Two antioxidative compounds were isolated and purified from the chloroform layer by Sephadex LH-20 column chromatography using the guided assay of DPPH radical scavenging. Based on mass spectrometer and nuclear magnetic resonance, the isolated compounds were identified as cirsiumaldehyde (1) and chrysoeriol (2). This is the first study to report the presence of those compounds in fermented glasswort juice. Compound 2 showed higher radical-scavenging activity than 1.

함초발효액의 기능성 해명과 유용 기능성 성분 탐색연구의 일환으로 함초발효액으로부터 항산화 활성물질을 분리하여 구조해석 하였다. 함초발효액을 용매분획 하여 얻어진 용매분획물들 중 $CHCl_3$층이 높은 항산화 활성을 나타냈다. 그래서 $CHCl_3$층을 Sephadex LH-20 column chromatography의 분자체 효과 및 흡착능의 특성을 각각 이용하여 정제함으로써 2종의 항산화 활성물질을 분리하였다. 단리한 이들 화합물을 대상으로 MS 및 NMR 분석을 통하여 cirsiumaldehyde(1)와 chrysoeriol(2)로 각각 동정하였다. 이 화합물들은 $ABTS^+$ 및 DPPH radical-scavenging 활성을 나타냈으며, 화합물 2는 화합물 1에 비해 더 높은 활성을 나타냈다.

Keywords

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