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Fabrication and Characterization of Ag-coated BCP Scaffold Derived from Sponge Replica Process

스폰지 복제법을 이용한 Ag 코팅 BCP 지지체의 제조 및 평가

  • Kim, Min-Sung (Department of Biomedical Engineering and Materials, College of Medicine, Soonchunhyang Univ.) ;
  • Kim, Young-Hee (Department of Biomedical Engineering and Materials, College of Medicine, Soonchunhyang Univ.) ;
  • Song, Ho-Yeon (Department of Biomedical Engineering and Materials, College of Medicine, Soonchunhyang Univ.) ;
  • Min, Young-Ki (Department of Biomedical Engineering and Materials, College of Medicine, Soonchunhyang Univ.) ;
  • Lee, Byong-Taek (Department of Biomedical Engineering and Materials, College of Medicine, Soonchunhyang Univ.)
  • 김민성 (순천향대학교 의과대학 의공학교실) ;
  • 김영희 (순천향대학교 의과대학 의공학교실) ;
  • 송호연 (순천향대학교 의과대학 의공학교실) ;
  • 민영기 (순천향대학교 의과대학 의공학교실) ;
  • 이병택 (순천향대학교 의과대학 의공학교실)
  • Received : 2010.07.13
  • Accepted : 2010.08.03
  • Published : 2010.08.27

Abstract

As a starting material, BCP (biphasic calcium phosphate) nano powder was synthesized by a hydrothermal microwave-assisted process. A highly porous BCP scaffold was fabricated by the sponge replica method using 60 ppi (pore per inch) of polyurethane sponge. The BCP scaffold had interconnected pores ranging from $100\;{\mu}m$ to $1000\;{\mu}m$, which were similar to natural cancellous bone. To realize the antibacterial property, a microwave-assisted nano Ag spot coating process was used. The morphology and distribution of nano Ag particles were different depending on the coating conditions, such as concentration of the $AgNO_3$ solution, microwave irradiation times, etc. With an increased microwave irradiation time, the amount of coated nano Ag particles increased. The surface of the BCP scaffold was totally covered with nano Ag particles homogeneously at 20 seconds of microwave irradiation time when 0.6 g of $AgNO_3$ was used. With an increased amount of $AgNO_3$ and irradiation time, the size of the coated particles increased. Antibacterial activities of the solution extracted from the Ag-coated BCP scaffold were examined against gram-negative (Escherichia coli) and gram-positive bacteria (Staphylococcus aureus). When 0.6 g of $AgNO_3$ was used for coating the Ag-coated scaffold, it showed higher antibacterial activities than that of the Ag-coated scaffold using 0.8 g of $AgNO_3$.

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