Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Poria Cocos on the Scopolamine-induced Memory Impairment and Its Underlying Molecular Mechanism

스코폴라민으로 유도된 기억력 손상에 대한 복신의 보호 효과 및 작용기전 연구

  • JeGal, Kyoung-Hwan (Department of Pathology, College of Oriental Medicine, Daegu Haany University) ;
  • Park, Sung-Jun (Department of Pathology, College of Oriental Medicine, Daegu Haany University) ;
  • Kim, Chang-Yul (Department of Pathology, College of Oriental Medicine, Daegu Haany University) ;
  • Lee, Chan (Department of Pathology, College of Oriental Medicine, Daegu Haany University) ;
  • Park, Jong-Hyun (Department of Pathology, College of Oriental Medicine, Daegu Haany University) ;
  • Jang, Jung-Hee (Department of Pathology, College of Oriental Medicine, Daegu Haany University)
  • 제갈경환 (대구한의대학교 한의과대학 병리학교실) ;
  • 박성준 (대구한의대학교 한의과대학 병리학교실) ;
  • 김창열 (대구한의대학교 한의과대학 병리학교실) ;
  • 이찬 (대구한의대학교 한의과대학 병리학교실) ;
  • 박종현 (대구한의대학교 한의과대학 병리학교실) ;
  • 장정희 (대구한의대학교 한의과대학 병리학교실)
  • Received : 2010.03.06
  • Accepted : 2010.04.08
  • Published : 2010.04.25
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Abstract

This study was performed to investigate the memory enhancing effect of Poria cocos Wolf (Hoelen cum radix) against scopolamine-induced amnesia in Sprague-Dawley (SD) rats. To induce amnesia, scopolamine (0.75 mg/kg) was intraperitonically injected into SD rats 30 min before starting behavior tests. We have conducted Morris water-maze and Y-maze tests to monitor learning and memory functions. Poria cocos effectively reversed scopolamine-induced memory impairment in SD rats which was represented by an improvement of mean escape latency in water-maze test and spontaneous alterations in Y-maze test. To elucidate possible molecule mechanism, we have measured mRNA as well as protein expression of acetylcholine esterase (AchE), choline acetyltransferase (ChAT), muscarinic acetylcholine receptor (mAchR), and brain-derived neurotrophic factor (BDNF) using RT-PCR and Western blot analysis, respectively. Poria cocos increased mRNA levels of ChAT and mAchR in rat hippocampus compared with those in the scopolamine-injected amnesic group. In addition, protein expression of ChAT and BDNF was also elevated by Poria cocos intake. Furthermore, as an upstrem regulator, the activation of cAMP response element-binding protein (CREB) was assessed by immunohistochemistry. In this immunohistochemical analysis, the phosphorylation of CREB (p-CREB) was reduced by scopolamine injection, which was restored back to control levels by administration of Poria cocos. These results suggest that Poria cocos may improve memory and cognitive deficit in amnesia and have therapeutic potentials through up-regulation of ChAT, mAchR, and BDNF, which seemed to be mediated by activation of CREB.

Keywords

References

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