Effects of Chestnut Inner Shell Extract on 3T3-L1 Preadipocyte Differentiation

율피 추출물이 3T3-L1 지방전구세포 분화에 미치는 영향

  • Lee, Seon-Goo (Department of Pathology, College of Oriental Medicine, Sangji University)
  • 이선구 (상지대학교 한의과대학 병리학교실)
  • Received : 2010.01.30
  • Accepted : 2010.04.12
  • Published : 2010.04.25

Abstract

Obesity occurred by energy imbalance, is increasing regardless of race, sex, age, and related to the metabolic syndrome, diabetes and cardiovascular disease. Since adipose tissue plays a critical role in regulating energy homeostasis, understanding of adipogenesis pathway and finding of regulatory mechanism for adipogenesis can be helpful to manage obesity as well as obesity-related diseases. In this study, to investigate the effects of Chestnut Inner Shell(CIS) extract on the adipogenesis in 3T3-L1 preadipocytes, 3T3-L1 preadipocytes were differentiated with adipogenic reagents for 9 days in the absence or presence of CIS extract ranging from 10 - 100 ${\mu}g/m{\ell}$. The effect of CIS extract on 3T3-L1 differentiation was examined by measuring intracelluar lipid droplet and triglyceride contents. CIS extract remarkably inhibited lipid accumulation(about 45% inhibition at 100 ${\mu}g/m{\ell}$ of CIS extract) and slightly decreased triglyceride contents(about 15% decrease at 100 ${\mu}g/m{\ell}$ of CIS extract) in 3T3-L1 preadipocytes at the concentration showing no cytotoxicity. These results demonstrated that CIS extract significantly inhibit adipogenesis and can be used for the regulation of obesity.

Keywords

References

  1. Ordovas, J.M., Mooser, V. Nutrigenomic and nutrigenetics. Curr Opin Lipidol 15: 101-108, 2004. https://doi.org/10.1097/00041433-200404000-00002
  2. Flegal, K.M., Carroll, M.D., Ogden, C.L., Johnson, C.L. Prevalence and trends in obesity among US adults, 1999-2000. JAMA 288(14):1723-1727, 2002. https://doi.org/10.1001/jama.288.14.1723
  3. Kereiakes, D.J., Willerson J.T. Metabolic syndrome epidemic. Circulation 108(13):1552-1553, 2003. https://doi.org/10.1161/01.CIR.0000093203.00632.2B
  4. Mokdad, A.H., Ford, E.S., Bowman, B.A., Dietz, W.H., Vinicor, F., Bales, V.S., Marks, J.S. Prevalence of obesity, diabetes and obesity related health risk factors, 2001. JAMA 289(1):76-79, 2003. https://doi.org/10.1001/jama.289.1.76
  5. Jee, S.H., Sull, J.W., Park, J., Lee, S.Y., Ohrr, H., Guallar, E., Samet, J.M. Body-mass index and mortality in Korean men and women. N Engl J Med 355(8):779-787, 2006. https://doi.org/10.1056/NEJMoa054017
  6. 배남규, 권인선, 조영채. 한국인의 10년간 비만수준의 변화 양상 : 1997-2007. 대한비만학회지 18(1):24-30, 2009.
  7. 조연지. 3T3-L1 아지방세포의 분화에 미치는 anthocyanins의 영향. 부경대학교 대학원, 2006.
  8. Yang, J.Y., Lee, K.S., Kim, M.K., Moon, S.K., Kang, M.K., Park, B.H., Kim, J.S., Park, J.W. Effect of acanthopanax senticosus on lipoprotein lipase in 3T3-L1 adipocytes. Phytother Res 18: 160-163, 2004. https://doi.org/10.1002/ptr.1413
  9. Sanders, S.R., Teachey, M.K., Ptock, A., Kraemer, K., Hasselwander, O., Henriksen, E.J., Baumgard, L.H. Effects of specific conjugated linoleic acid isomers on growth characteristics in obese Zucker rats. Lipids 39: 537-543, 2004. https://doi.org/10.1007/s11745-004-1260-0
  10. Ahima, R.S., Flier, J.S. Adipose tissue as an endocrine organ. Trend Endocrinol Metab 11(8):327-332, 2000. https://doi.org/10.1016/S1043-2760(00)00301-5
  11. 김만조, 이 욱, 황명수, 김선창, 이문호. 밤나무 재배품종의 개화, 결실 및 과실특성. 한국임학회지 92(4):321-322, 2003.
  12. 손철호, 석현덕, 민경택. 국내 밤산업의 동향과 전망. 농촌경제연구소. pp 77-79, 2003.
  13. Choi, O.B., Kim, K.M., Yoo, G.S., Park, K.H. Anti-allergic effect of Castanea crenata leaf tea. Korean J Food Sci Technol 30: 468-471, 1998.
  14. Kwon, E.J., Kim, Y.C., Kwon, M.S., Kim, C.S., Kang, W.W., Lee, J.B., Chung, S.K. Antioxidative activity of solvent fraction and isolation of antioxidant compound from chestnut husk. J Korean Soc Food Sci Nutr 30: 726-731, 2001.
  15. Kim, S.K., Jeon, Y.J., Kim, Y.T., Lee, B.J., Kang, O.J. Sensory evaluation and retrogradation properties of chestnut mook. J Korean Soc Food Nutr 24: 601-605, 1995.
  16. Park, Y.H., Kim, S.K., Lee, S.Y., Kim, J.B. Rheological properties of gelatinized chestnut starch solution. Korean J Food Sci Technol 16: 314-318, 1984.
  17. Cho, S.H., Sung, N.K., Ki, W.K., Hur, J.H., Shim, K.H., Chung, D.H. Effect of blanching on the prevention of discoloration in the thermal-treated chestnut power. J Korean Soc Food Nutr 17: 211-214, 1988.
  18. Yoon, W.J. The study on the humidity preserving effect with several natural packs. Dong Duck Women`s University. 1997.
  19. Oh, S.H., Kim, Y.W., Kim, M.A. The antioxidant activities of acetone extract of chestnut inner shell, Pine needle and Hop. Korean J. Food Culture 19(4):339-406, 2004.
  20. 정수현 외 12명. 율피의 생리활성 및 미백효과를 이용한 화장품 신조재에 관한 연구. 대한본초학회지 20(2):27-33, 2005.
  21. 이현주, 정미자, 조재열, 함승시, 최 면. 국내산 밤 일부 품종의 기능성 성분분석과 항산화 및 대식세포 활성. J Korean Soc Food Sci Nutr 37(9):1095-1100, 2008. https://doi.org/10.3746/jkfn.2008.37.9.1095
  22. Hsu, C.L., Yen, G.C. Effect of gallic acid on high fat diet-induced dyslipidaemia, hepatosteatosis and oxidative stress in rats. British journal of Nutrition 98: 727-735, 2007.
  23. 이순재, 김경란, 김홍태, 홍정희. 고지방 식이로 유도된 비만 쥐에서 녹차 Catechin이 체지방 조성 및 지방조직에 미치는 영향. J Korean Soc Sci Nutr 36(5):540-547, 2007. https://doi.org/10.3746/jkfn.2007.36.5.540
  24. 김미자, 김영옥, 정주호, 김종우, 김혜경. Caffeine이 지방세포주 3T3-L1 분화에 미치는 영향 : 영양유전체학적 접근. 한국영양학회지 38(8):649-655, 2005.
  25. 허 준. 동의보감. 서울, 법인문화사, p 3449, 2005.
  26. Noh, J.R., Gang, G.T., Moon, J.S., Kim, Y.H., Yang, K.J., Choi, D.H., Oh, W.K., Song, K.S., Lee, C.H. Antioxidant Effects of Chestnut Inner Shell Extract on High Fat-diet fed C57BL/6 Mice and t-BHP treat HepG2 cells. Symposium of the Korean Association for Labotatory Animal Science p 206, 2009.
  27. Noh, J.R., Gang, G.T., Moon, J.S., Kim, Y.H., Yang, K.J., Choi, D.H., Oh, W.K., Song, K.S., Lee, C.H. Hypolipidemic Effects of Chestnut Inner Shell Extract on High Fat-diet fed C57BL/6 Mice. Symposium of the Korean Association for Labotatory Animal Science p 205, 2009.
  28. 양민진, 김명길, 임세진, 안형수, 안령미. 율피.솔잎.호프의 수성 아세톤 추출물에 의한 Melanin 생성 억제 효과. 약학회지 43(4):494-501, 1999.
  29. 진동언, 김관천. 율피 추출물이 티로시나아제 유전자 발현에 미치는 효과. 대한위생학회지 20(3):10-16, 2005.
  30. 진휘승, 박금주, 박승희, 김재기. 항비만에 대한 생약복합물의 효과. 한국식품영양학회지 38(1):32-38, 2009. https://doi.org/10.3746/jkfn.2009.38.1.032
  31. Ailhaud, G., Grimaldi, P., Negrel, R. Cellular and molecular aspects of adipose tissue development. Annu Rev Nutr 12: 207-233, 1992. https://doi.org/10.1146/annurev.nu.12.070192.001231
  32. Haugen, F., Zahid, N., Dalen, K.T., Hollung, K., Nebb, H.I., Drevon, C.A. Resistin expression in 3T3-L1 adipocytes is reduced by arachidonic acid. Lipid Res 46: 143-153, 2005. https://doi.org/10.1194/jlr.M400348-JLR200
  33. Tenney, R., Stansfield, K., Oekala, P.H. Interleukin 11 signaling in 3T3-L1 adipocytes. J cell physiol 202: 160-166, 2005. https://doi.org/10.1002/jcp.20100
  34. Guo, X., Liao, K. Analysis gene expression profile during 3T3-L1 preadipocytes differentiation. Gene 251: 45-53, 2000. https://doi.org/10.1016/S0378-1119(00)00192-X
  35. MacDougald, O.A., Hwang, C.S., Fan, H., Lane, M.D. Regulated expression of the obese gene product(leptin) in white adipose tissue and 3T3-L1 adipocytes. Proc Natl Acad Sci USA. 92(20):9034-9037, 1995. https://doi.org/10.1073/pnas.92.20.9034
  36. Klaus, S. Adipose tissue as a regulator of energy balance. Curr Drug Targets, 5(3):241-250, 2004. https://doi.org/10.2174/1389450043490523
  37. Kim, J.B., Spiegelman, B.M. ADD1/SREBP1 promotes adipocyte differentiation and gene expression linked to fatty acid metabolism. Gene & Development 10: 1096-1107, 1996. https://doi.org/10.1101/gad.10.9.1096
  38. Koldovsky, O., Dobiasova, M., Drahota, Z., Hahn, P. Developmental aspects of lipid metabolism. Physiol Res 44(6):353-356, 1995.