Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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Antioxidative and Anti-inflammatory Effects of Aurantii Fructus Immaturus Pharmacopuncture

지각 약침액의 항산화 및 항염증 효과에 관한 연구

  • Kim, Sung-Jin (Dept. of Meridian & Acupoint, College of Oriental Medicine, Sangji University) ;
  • Park, Sang-Kyun (Dept. of Meridian & Acupoint, College of Oriental Medicine, Sangji University)
  • 김성진 (상지대학교 한의과대학 경락경혈학교실) ;
  • 박상균 (상지대학교 한의과대학 경락경혈학교실)
  • Received : 2010.03.18
  • Accepted : 2010.06.09
  • Published : 2010.06.27
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Abstract

Objectives : Ulcerative colitis is a chronic relapsing inflammatory disease in the gastrointestinal tract. We investigated whether Aurantii fructus immaturus (AFI) pharmacopuncture has antioxidative and anti-inflammatory effects. Methods : in vitro experiments, 1,1-diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging activity, superoxide dismutase (SOD) activity, prevention on $H_2O_2$-induced cell death in RAW264.7 cell line, DNA fragmentation, and cyclooxygenase-2 mRNA expression induced by lipopolysaccharide (LPS), were analyzed to investigate antioxidative and anti-inflammatory effect of AFI pharmacopuncture. in vivo experiment, a murine model of dextran sulfate sodium (DSS)-induced colitis was used to examine the effect of AFI pharmacopuncture on CV12 at different doses of 5 ${\mu}l$, 0.5 ${\mu}l$, 0.05 ${\mu}l$ for 10 days. Body weight, colon length and macroscopic features were investigated. Results : AFI pharmacopuncture showed DPPH free radical scavenging and SOD active effects in a dose-dependent manner. AFI pharmacopuncture showed a protective effect against $H_2O_2$-induced cell injury and also attenuated LPS-induced COX-2 mRNA expression. In a DSS- induced colitis murine model, however, AFI pharmacopuncture at CV12 had no anti-inflammatory effects. Conclusions : The present results suggest that AFI pharmacopuncture extract may have anti- inflammatory and antioxidative effects in vivo test, but further research on the underlying mechanism is required.

Keywords

References

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