Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
권호 표지

Immunomodulatory activity of cultivated wild ginseng pharmacopuncture

산양산삼약침의 면역조절기능

  • Kim, Young-Jin (Dept. of Merdian & Acupoint, College of Oriental Medicine, Sangji University) ;
  • Lee, Joon-Moo (Dept. of Merdian & Acupoint, College of Oriental Medicine, Sangji University) ;
  • Lee, Eun (Dept. of Pharmaceutical Engineering, College of Health Sciences, Sangji University)
  • 김영진 (상지대학교 한의학과 경락경혈학교실) ;
  • 이준무 (상지대학교 한의학과 경락경혈학교실) ;
  • 이은 (상지대학교 보건과학대학 제약공학과)
  • Received : 2010.02.22
  • Accepted : 2010.03.23
  • Published : 2010.03.27
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Abstract

Objectives: To investigate the anti-inflammatory effects of cultivated wild ginseng pharmacopuncture in lipopolysaccharide (LPS)-induced inflammatory rat model. Methods: Sprague-Dawley rats were divided into 4 groups; LPS control (n=6), LPS+cultivated wild ginseng pharmacopuncture at CV4 (n=6), LPS+cultivated wild ginseng pharmacopuncture at CV17 (n=6), and LPS+cultivated wild ginseng pharmacopuncture at Ex-HN1 (n=6). Pharmacopuncture (0.1 ml) was given every two days for 4 weeks followed by inflammation induction by peritoneal LPS injection (5 mg/kg). Blood, liver tissue, and peritoneal lavage fluid were taken and proinflammatory cytokines and other related factors were analysed. Results: Compared with the control group, CV4 and Ex-HN1 pharmacopuncture groups significantly attenuated plasma IL-$1{\beta}$, IL-6, and TNF-$\alpha$ increase at 2h and 5h after LPS injection (P<0.05). A significant difference from control group emerged at 5 h for plasma IL10 (P<0.05). For liver cytokines analyzed at 5 h after LPS injection, only CV4 pharmacopuncture group showed significant difference in TNF-$\alpha$ and IL-10 (P<0.05). Blood CD4/CD8 ratio and the phagocytic activities of polymorphonuclear neutrophils were not different from those of control group in all pharmacopuncture groups (P>0.05). CV4 pharmacopuncture significantly attenuated increase of plasma ${NO_3}^-/{NO_2}^-$, Intracellular adhesion molecule-1 (ICAM-1), cytokine-induced neutrophil chemoattractant-1 (CINC-1), and prostaglandin $E_2$ ($PGE_2$) compared with the control group (P<0.05). Monocyte chemoattractant protein-1, $PGE_2$, and CINC-1 level of CV4 pharmacopuncture group was significantly different from those from the control group (P<0.05). Conclusions: These results indicate that cultivated wild ginseng pharmacopuncture at CV4 may have a potent anti-inflammatory effect in an LPS-induced inflammatory rat model.

Keywords

References

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