Effects of Rehmanniae Radix on HPA Axis System and Catecholamienrgic System in FST

생지황(生地黃)이 강제수영부하시험에서 HPA Axis System과 Catecholaminergic System에 미치는 영향

  • Park, Jae-Beom (Dept. of Formulae Pharmacology, College of Oriental Medicine, Kyungwon University) ;
  • Lee, Tae-Hee (Dept. of Formulae Pharmacology, College of Oriental Medicine, Kyungwon University)
  • 박재범 (경원대학교 한의과대학 방제학교실) ;
  • 이태희 (경원대학교 한의과대학 방제학교실)
  • Received : 2010.04.02
  • Accepted : 2010.06.17
  • Published : 2010.06.30

Abstract

Object : The effects of Rehmanniae Radix(RR) on HPA Axis system and Catecholaminergic system was investigated. Methods : we performed the Forced Swimming Test(FST). Also the expression of Corticotropin -Releasing Factor(CRF), c-Fos and Tyrosine Hydorxylase(TH) was measured by immunohistochemical method at Paraventricular Nucleus(PVN), Locus Coeruleus(LC) and Ventral Tegmental Area(VTA). Results : 1. The duration of immobility in the Forced Swimming Test was significantly decreased in the Rehmanniae Radix 400 mg/kg groups in comparison with the control group(p<0.05). 2. Comparing to the control group, CRF expression was significantly decreased in the Rehmanniae Radix 400 mg/kg treated group in comparison with the control group(p<0.01). 3. c-Fos expression was significantly decreased at PVN in the Rehmanniae Radix 100 mg/kg treated group in comparison with the control group(p<0.01). 4. TH expression was significantly increased at LC in the Rehmanniae Radix 100 mg/kg and 400 mg/kg treated group in comparison with the control group(p<0.001). 5. TH expression was significantly decreased at VTA in the Rehmanniae Radix 100 mg/kg and 400 mg/kg treated group in comparison with the control group(p<0.001). Conclusion : According to the results, it can be considered that Rehmanniae Radix has antidepressant effect by showing the reduction of immobility in FST through the decreased expression of CRF, c-Fos in PVN and TH in VTA.

Keywords

References

  1. 허준. 동의보감. 서울:법인문화사. 2005:1127.
  2. 이천. 의학입문. 서울:한성사. 1994:409, 534.
  3. 신민교. 임상본초학. 서울:영림사. 1986:248-54.
  4. 강병수 등. 본초학. 서울:영림사. 1991:190-2.
  5. 유경수. 지황의 수치에 관한 연구. 경희대학교논문집. 1965;4: 233-252.
  6. 黃宮綉. 本草求眞. 北京:人民衛生出版社. 1987:214.
  7. 吳儀洛. 本草從新. 서울:杏林書院. 1972:46-7.
  8. 조용국, 이태희. 柴胡抑肝湯이 스트레스로 인한 기억저하와 우울행동에 미치는 영향. 대한한의학방제학회지. 2007;15(2):147-60.
  9. 권용욱, 이태희. 청피가 FST에서 나타나는 우울행동에 미치는 영향. 경원대학교. 2008;34.
  10. 고동균, 이태희. 지골피가 스트레스로 인한 기억저하와 우울행동에 미치는 영향. 대한본초학회지. 2007;22:137-46.
  11. Arthur H Swiergiel. Yueping Zhou, Adrian J Dunn. Effects of chronic footshock, restraint and corticotropin-releasing factor on freezing, ultrasonic vocalization and forced swimming behavior in rats. Behavioral Brain Research. 2007;183:178-87. https://doi.org/10.1016/j.bbr.2007.06.006
  12. Gustavo E, Tafet, Renato Bernardini. Psychoneuroendocrinological links between chronic stress and depression. Progress in Neuro- Psychopharmacology & Biological Psychiatry. 2003;27:893-903. https://doi.org/10.1016/S0278-5846(03)00162-3
  13. G. Garcia P, Tagliaferro A, Ferri Am. Evangelista de Duffard, R Duffard, A Bruso. Study of Tyrosine Hydroxylase Immunoreactive Neurons in Neonate Rats Latationally Exposed to 2, 4-Dichlorophenoxyacetic Acid. Neurotoxicology. 2004;25:951-7. https://doi.org/10.1016/j.neuro.2004.05.004
  14. 강형원 등. 한방신경정신의학. 서울:집문당. 2006:146, 256-7.
  15. 김종우 등. stress의 한의학적 이해. 동의신경정신과학회지 1993;4(1):19-26.
  16. 임영환 등. 교감단의 기울증 치료에 관한 문헌연구:수승화강을 중심으로. 방제학회지. 2008;16(1):1-13.
  17. 문충모, 김지혁, 황의원. 히스테리에 관한 임상적 고찰. 경희한의대논문집. 1985;8:81-9.
  18. 김현준 등. 시호억간탕 등 약물의 전 처치가 구속 스트레스로 유발된 생쥐의 혈중 Corticosterone 변화에 미치는 영향. 대한한의학방제학회지. 2002;10(2):57-72.
  19. 안수진 등. 구속스트레스로 유발된 생쥐 시상하부내 단가아민의 변화에 대한 시호소간산과 시호억간탕의 효능. 대한한의학방제학회지. 2003;11(1):99-114.
  20. 박미순 등. 시호억간탕의 항stress 효능에 대한 연구. 대한한방부인과학회지. 2000;13(1):219-61.
  21. 황영명. 생지황 건지황 숙지황이 세포성 면역 반응 및 체액성면역반응에 미치는 영향. 사상의학회지. 1989;1(1):139-51.
  22. 신광식 등. 생지황 추출물이 DNCB로 유도된 생쥐의 allergy성 접촉피부염에 미치는 영향. 대한방제학회지. 2000;8(1):257-79.
  23. Kato M. Focal cerebral ischemia-induced escape deficit in rats is ameliorated by a reversible inhibitor of monoamine oxidase-a, implications for a novel animal model of post-stroke depression, Biol. Pharm. Bull. 2000;23(4):406-10. https://doi.org/10.1248/bpb.23.406
  24. John F. Cryan, Rita J. Valentino, Irwin Lucki. Assessing substrates underlying the behaviroal effects of antidepressants using the modified rat forced swimming test. Neuroscience and Biobehaviroal Reviews. 2005;29:547-69. https://doi.org/10.1016/j.neubiorev.2005.03.008
  25. 신화석, 권영모, 박선동. 四逆散의 抗憂鬱效果 및 腦內 monoamine에 미치는 영향. 대한본초학회지. 2004;19(2):71-82.
  26. 홍성유, 박선동. 柴胡가 우울증 모델 백서의 catecholamine에 미치는 영향. 대한본초학회지. 2003;18(4):245-53.
  27. Herman JP, Cullinan WE. Neurocircuitry of stress: central control of the hypothalamo -pituitary-adrenocorticoal axis. Trends Neurosci. 1997;20(2):78-84. https://doi.org/10.1016/S0166-2236(96)10069-2
  28. Campbell BM, Morrison JL, Walker EL, Merchant KM. Differential regulation of behavioral, genomic and neuroendocrine responses by CRF infusions in rats. Pharmacology Biochemistry and Behavior. 2004;77(3):447-55. https://doi.org/10.1016/j.pbb.2003.12.010
  29. Boutillier A. L. Monnier D. Lorang D. Lundblad J. R. Roberts J. L. Loeffler J. P. Corticotropin -releasing hormone stimulates proopiomelanocortin transcription by cFos-dependent and -independent pathway:Characterization of an AP-1 site in exon 1. Mol. endocrinol. 1995;5:1301-10.
  30. Dominnic J. Autelitano. Stress-induced Stimulation of Pituitary POMC gene Expression is Associated with Activation of Transcription Factor AP-1 in Hypothalamus and Pituitary. Brain Research bulletin 1998;45(1):75-82. https://doi.org/10.1016/S0361-9230(97)00303-1
  31. Doucet JP, Squinto SP, Bazan NG. Fos-jun and the primary genomic response in the nervous system. Mol Neurobiol. 1990;4:27-55. https://doi.org/10.1007/BF02935584
  32. Helmreich DL, Cullinan WE, Watson SJ. The effect of adrenalectomy on stress-induced c-Fos mRNA expression in the rat brain. Brain Res. 1996;706:137-44. https://doi.org/10.1016/0006-8993(95)01215-X
  33. Sapolsky RM, Krey LC, McEwen BS. Glucocorticoid -sensitive hippocampal neurons are involved in terminating the adrenocortical stress response. Proc Natl Acad Sci. 1984;81: 6174-7. https://doi.org/10.1073/pnas.81.19.6174
  34. Hahn MK, Bannon MJ. Stress-induced c-Fos expression in the rat locus coeruleus is dependent on neurokinin 1 receptor activation. Neuroscience. 1999;94(4):1183-8. https://doi.org/10.1016/S0306-4522(99)00319-X
  35. Laorden ML, Castells MT, Milanés MV. Effects of U-50, 488H and U-50, 488H withdrawal on c-Fos expression in the rat paraventricular nucleus Correlation with c-Fos in brainstem catecholaminergic neurons. British Journal of Pharmacology. 2003;138:1544-52. https://doi.org/10.1038/sj.bjp.0705179
  36. 조충훈. 청화보심탕이 우울증 모형동물의 절망 행동, 불안 및 뇌의 TH와 c-Fos 발현에 미치는 효과. 동의신경정신과학회지. 2003;14(2):61-78.
  37. 정선용 등. 황련해독탕이 우울증 모형 동물의 우울성향 및 PVN의 c-Fos 발현에 미치는 효과. 동의신경정신과학회지. 2003;14(1):1-16.
  38. Cervo L, Bendotti C, Tarizzo G, Cagnotto A, Skorupska M, Mennini T, Samanin R. Potential antidepressant properties of SR 57746A, a novel compound with selectivity and high affinity for 5-HT(1A) receptors. Eur. J. Pharmacol. 1994;253:139-47. https://doi.org/10.1016/0014-2999(94)90768-4
  39. Wang P, Kitayama I, Nomura J. Tyrosine hydroxylase gene expression in the locus coeruleus of depression-model rats and rats exposed to short- and long-term forced walking stress. Life Sci. 1998;62(23):2083-92. https://doi.org/10.1016/S0024-3205(98)00183-0
  40. Nishmura H, Tanaka M. Effects of alprazolam on anxiety-related behavior of rats in a modified forced-swim test employing straw suspension. Pharmac. Biochm. Behav. 1992;41:425-7. https://doi.org/10.1016/0091-3057(92)90121-U
  41. Komori T, Nomura J, Inoue K, Kitayama I. Tyrosine hydroxylase activity in discreste brain regions of depression model rats. Jpn. J. Psychiarty Neurol. 1990;44(4):747-54.
  42. Mokha, SS, JA Mcmillan and A. lggo. Descending control of spinal nociceptive transmission. Actions produced on spinal multireceptive neurones from the nuclei locus coeruleus(LC) and raphe magnus(NRM). Exp. Brain. Res. 1985;58:213-6.
  43. Nakazato, T. Locus coeruleus neurons projecting to the forebrain and the spinal cord in the cat. Neuroscience. 1987;23:529-38. https://doi.org/10.1016/0306-4522(87)90074-1
  44. Crossman AR, Neary D. Neuroanatomy. New York:Churchill Livingstone. 1996:65-69, 121-2.
  45. Ortiz J, Fitzgerald LW, Lane S, Terwilliger R, Nestler EJ. Biochemical adaptations in the mesolimbic dopamine system in response to repeated stress. Neuropsychopharmacology. 1996 ;14(6):443-52. https://doi.org/10.1016/0893-133X(95)00152-4
  46. Paxinos G, Watson C. The Rat Brain in Stereotaxic Coordinates. New York:Academic Press. 1986.