Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Monitoring Ochratoxin A in Coffee and Fruit Products in Korea

커피 및 과실류 가공품의 오크라톡신 A 모니터링

  • Park, Ji-Eun (Imported Food Analysis Division, Center for Food and Drug Analysis, Gyeongin Regional Korea Food and Drug Administration) ;
  • Heo, Seok (Imported Food Analysis Division, Center for Food and Drug Analysis, Gyeongin Regional Korea Food and Drug Administration) ;
  • Lee, Mi-Seon (Imported Food Analysis Division, Center for Food and Drug Analysis, Gyeongin Regional Korea Food and Drug Administration) ;
  • Kim, Eun-Jung (Imported Food Analysis Division, Center for Food and Drug Analysis, Gyeongin Regional Korea Food and Drug Administration) ;
  • Park, Jong-Seok (Imported Food Analysis Division, Center for Food and Drug Analysis, Gyeongin Regional Korea Food and Drug Administration) ;
  • Oh, Jae-Ho (Imported Food Analysis Division, Center for Food and Drug Analysis, Gyeongin Regional Korea Food and Drug Administration) ;
  • Jang, Young-Mi (Imported Food Analysis Division, Center for Food and Drug Analysis, Gyeongin Regional Korea Food and Drug Administration) ;
  • Kim, Mee-Hye (Imported Food Analysis Division, Center for Food and Drug Analysis, Gyeongin Regional Korea Food and Drug Administration)
  • 박지은 (경인지방식품의약품안전청 시험분석센터 수입식품분석과) ;
  • 허석 (경인지방식품의약품안전청 시험분석센터 수입식품분석과) ;
  • 이미선 (경인지방식품의약품안전청 시험분석센터 수입식품분석과) ;
  • 김은정 (경인지방식품의약품안전청 시험분석센터 수입식품분석과) ;
  • 박종석 (경인지방식품의약품안전청 시험분석센터 수입식품분석과) ;
  • 오재호 (경인지방식품의약품안전청 시험분석센터 수입식품분석과) ;
  • 장영미 (경인지방식품의약품안전청 시험분석센터 수입식품분석과) ;
  • 김미혜 (경인지방식품의약품안전청 시험분석센터 수입식품분석과)
  • Received : 2009.11.03
  • Accepted : 2010.01.21
  • Published : 2010.06.30
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Abstract

This research was conducted to evaluate the occurrence of ochratoxin A (OTA) in coffee and fruit products in Korea. A total of 388 coffee and fruit product samples were collected from retail or outlet markets; 177 samples were coffee and 211 were fruits or their products. Analytical methods including AOAC and Comit$\acute{e}$ Europ$\acute{e}$en de Normalisation (CEN) were selected and modified by method validation to detect and quantify the OTA in samples. All samples were analyzed by liquid chromatography with fluorescence detection. OTA was detected in 3.9% of 177 kinds of coffee and 0% of 211 kinds of fruit products. The levels of OTA were $0.7-4.6\;{\mu}g/kg$ in green coffee, $0.3-4.8\;{\mu}g/kg$ in roasted coffee, $1.4\;{\mu}g/kg$ in mixed coffee, and $0.4-0.6\;{\mu}g/kg$ in instant coffee. However, OTA was not detected in liquid coffee, dried fruits, or grape juice. OTA levels of all samples detected were less than the European Union legislation of $5.0\;{\mu}g/kg$ in coffee, $10.0\;{\mu}g/kg$ in raisins and $2.0\;{\mu}g/kg$ in grape juice. Therefore, the risk of OTA in coffee and fruit products in Korea is relatively low at safe levels.

국내 커피 및 과실류 가공품 중 오크라톡신 함량 조사를 위해 전처리법과 분석법을 확립하여 커피와 과실류 가공품 총 388건을 모니터링한 결과 7건에서 오크라톡신이 검출되었다. 커피 177건 중 7건에서 검출되었고, 평균 함량은 $0.139\;{\mu}g/kg$으로 EU 기준 $5.0\;{\mu}g/kg$ 보다 낮아 안전한 수준으로 판단되었다. 과실류 가공품에서는 211건 모두 오크라톡신이 검출되지 않았다. 이상에서 살펴본 바와 같이 커피 및 과실류 가공품의 오크라톡신의 오염수준은 전체적으로 높지 않지만, 최근 기호 식품으로서 커피 및 과실류 가공품의 섭취가 점차 늘어나고 있고, 오크라톡신 오염량이 비교적 높은 커피를 선호하는 계층에 있어서는 오크라톡신에 지속적으로 노출될 가능성이 있으므로 주의해야 할 것으로 생각되며, 앞으로도 이들에 대한 체계적인 관리와 안전성 확보를 위한 지속적인 노력이 필요할 것으로 생각된다.

Keywords

References

  1. Nicholas WT, Sreenath S, Sergey AP. Analytical methods for determination of mycotoxins. Anal. Chem. Acta 632: 168-180 (2008)
  2. Chung SH. The monitoring of Ochratoxin in food commodities, Annu. Rep. KFDA. Korea 10: 673 (2006)
  3. Walker R. Mycotoxins of growing interest: Ochratoxins, Third joint FAO/WHO/UNEP international conference on mycotoxins, Tunis, Tunisia (1999)
  4. Krogh P, Elling F, Hald B, Jyling B, Petersen VE, Skadhauge E, Svendsen CK. Experimental avian nephropathy. Changes of renal function and structure induced by ochratoxin A-contaminated feed. Acta Pathol. Mic. Sc. 84: 215-221 (1976)
  5. Singh GS, Chauhan HV, Jha GJ, Singh KK. Immunosuppression of chronic ochratoxincosis in broiler chicks. J. Comp. Pathol. 130: 399-410 (1990)
  6. Krogh P, Gyrd-Hansen N, Hald B, Larsen S, Nielsen JP, Smith M, Ivanoff C, Meisner H. Renal enzyme activities in experimental ochratoxin A-induced porcine nephropathy: diagnostic potential of phosphoenolpyruvate carboxykinase and gamma-glutamyl transpeptidase activity, J. Toxicol. Env. Health 23: 1-4 (1988) https://doi.org/10.1080/15287398809531092
  7. Page RK, Stewart G, Wyatt R, Bush P, Fletcher OJ, Brown J. Influence of low levels of ochratoxin A on egg production, eggshell stains, and serum uric-acid levels in Leghom-type hens. Avian Dis. 24: 777-780 (1980) https://doi.org/10.2307/1589815
  8. Van der Merwe KJ, Steyn PS, Fourie L, Scott DB, Theron JJ. Ochratoxin A, a toxic metabolite produced by Aspergillus ochraceus. Nature 205: 1112-1113 (1965) https://doi.org/10.1038/2051112a0
  9. Casegnaro M, Wild CP. IARC activities in mycotoxin research. Nat. Toxins 3: 327-331 (1995) https://doi.org/10.1002/nt.2620030431
  10. IARC. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans: Some Naturally Occurring Substances, Food Items and Constituents, Heterocyclic Amines and Mycotoxins. International Agency for Research on Cancer, Lyon, France. pp. 489-521 (1993)
  11. Birzele B, Prange A, Kramer J. Deoxynivalenol and ochratoxin A in German wheat and changes of level in relation to storage parameters. Food Addit. Contam. 17: 1027-1035 (2000) https://doi.org/10.1080/02652030050207828
  12. Curtui VG, Gareis M, Usleber E, Martlbauer E. Survey of Romanian slaughtered pigs for the occurrence of mycotoxins ochratoxins A and B, and zearalenone. Food Addit. Contam. 18: 730-738 (2001)
  13. Czerwiecki L, Czajkowska D, Witkowska-Gwiazdowska A. On ochratoxin A and fungal flora in Polish cereals from conventional and ecological farms. Part 2: Occurrence of ochratoxin A and fungi in cereals in 1998. Food Addit. Contam. 19: 1051-1057 (2002) https://doi.org/10.1080/02652030210156313
  14. Jorgensen K, Petersen A. Content of ochratoxin A in paired kidney and meat samples from healthy danish slaughter pigs. Food Addit. Contam. 19: 562-567 (2002) https://doi.org/10.1080/02652030110113807
  15. Park JW, Chung SH, Kim YB. Ochratoxin A in Korean food commodities: occurrence and safety evaluation. J. Agr. Food Chem. 53: 4637-4642 (2005) https://doi.org/10.1021/jf050227j
  16. Park JW, Kim EK, Shon DH, Kim YB. Natural co-occurrence of aflatoxin B1, fumonisin B1, and ochratoxin A in barley and corn foods from Korea. Food Addit. Contam. 19: 1073-1080 (2002) https://doi.org/10.1080/02652030210151840
  17. Blanc M, Pittet A, Munoz-Box R, Viani R. Behavior of ochratoxin A during green coffee roasting and soluble coffee manufacture. J. Agr. Food Chem. 46: 673-675 (1998) https://doi.org/10.1021/jf9707703
  18. Lombaert GA, Pellaers P, Chettiar M, Lavalee D, Scott PM, Lau BP. Survey of canadian retail coffees for ochratoxin A. Food Addit. Contam. 19: 869-877 (2002) https://doi.org/10.1080/02652030210145027
  19. Fazekas B, Tar AK, Zomborszky-Kovacs M. Ochratoxin A contamination of cereal grains and coffee in Hungary in the year 2001. Acta Vet. Hung. 50: 177-188 (2001)
  20. Santos EA, Vargas EA. Immunoaffinity column clean-up and thin layer chromatography for determination of ochratoxin A in green coffee. Food Addit. Contam. 19: 447-458 (2002) https://doi.org/10.1080/02652030110213717
  21. Chan D, MacDonald SJ, Boughtflower V, Bereton P. Simultaneous determination of aflatoxin and ochratoxin A in food using a fully automated immunoaffinity column clean-up and liquid chromatography-fluorescence detection. J. Chromatogr. A 1059: 13-16 (2004) https://doi.org/10.1016/j.chroma.2004.09.096
  22. Entwisle AC, Willians AC, Mann PJ, Slack PT, Gilbert J. Liquid chromatographic method with immunoaffinity column cleanup for determination of ochratoxin A in barley: Collaborative study. J. AOAC Int. 83: 1377-1383 (2000)
  23. AOAC. Ochratoxin A in Roasted Coffee Immunoaffinity Column HPLC Method, AOAC Official Method 2000.09 (2000)
  24. AOAC. Ochratoxin A in Green Coffee Immunoaffinity Column Cleanup and Liquid Chromatography. AOAC Official Method 2004.10 (2004)
  25. Alain P, Dominique T, Anthony H, Rinantonio V. Liquid chromatographic determination of ochratoxin A in pure and adulterated soluble coffee using an immunoaffinity column cleanup procedure. J. Agr. Food Chem. 44: 3564-3569 (1996) https://doi.org/10.1021/jf9602939
  26. Entwisle AC, Willians AC, Mann PJ, Slack PT, Gilbert J. Combined phenyl silane and immunoaffinity column cleanup with liquid chromatographic for determination of ochratoxin A in roasted coffee: Collaborative study. J. AOAC Int. 84: 444-450 (2001)
  27. Lau BP, Scott PM, Lewis DA, Kanhere SR. Quantitative determination of ochratoxin A by liquid chromatographic/electrospray tandem mass spectrometry. J. Mass Spectrom. 35: 23-32 (2000) https://doi.org/10.1002/(SICI)1096-9888(200001)35:1<23::AID-JMS903>3.0.CO;2-B
  28. EC. Assessment of dietary intake of Ochratoxin A by the population of EU Member states. Report of experts participating in Task3.2.7. EU, January (2002)
  29. Yoshiko SK, Masahiro N, Setsuko T, Eiichi I, Toshitsugu T, Hiroko N, Yoshinori I, Koji A, Kazuhiro F, Shigeni K, Susumu K. Occurrence of aflatoxins, ochratoxin A, and fumonisins in retail foods in Japan. J. Food Protect. 69: 1365-1370 (2006)
  30. Czerwiecki L, Wilczynska G, Kwiecien A. Ochratoxin A: An improvement clean-up and HPLC method used to investigate wine and grape juice on the Polish market. Food Addit. Contam. 22: 158-162 (2005) https://doi.org/10.1080/02652030500038066
  31. Korean Agency for Technology and Standards. Size Korea Report. 5th. Ministry of Commerce, Industry and Energy, Seoul, Korea. pp. 1-300 (2004)
  32. Korea Health Industry Development Institute. The Third Korea National Health & Nutrition Examination Survey: Nutrition Survey (II). Ministry of Health and Welfare, Seoul, Korea (2006)
  33. WHO. The Fifty-sixth meeting of Joint FAO/WHO expert committee on food additives (JECFA), Safety evaluation of certain mycotoxins in food. WHO Food Additive Series. 47: 281-415 (2000)