Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Immunomodulating Activity of Laminaria japonica Polysaccharides

참다시마 다당체의 면역 증강 활성

  • Ryu, Deok-Seon (Department of Biomedical Laboratory Science, Inje University) ;
  • Oh, Seung-Min (Department of Biomedical Laboratory Science, Inje University) ;
  • Kim, Ki-Hoon (Department of Biomedical Laboratory Science, Inje University) ;
  • Kim, Soo-Hwan (Department of Biomedical Laboratory Science, Inje University) ;
  • Choi, Hyun-Ju (Department of Biomedical Laboratory Science, Inje University) ;
  • Lee, Dong-Seok (Department of Biomedical Laboratory Science, Inje University)
  • 류덕선 (인제대학교 의생명공학대학 임상병리학과) ;
  • 오승민 (인제대학교 의생명공학대학 임상병리학과) ;
  • 김기훈 (인제대학교 의생명공학대학 임상병리학과) ;
  • 김수환 (인제대학교 의생명공학대학 임상병리학과) ;
  • 최현주 (인제대학교 의생명공학대학 임상병리학과) ;
  • 이동석 (인제대학교 의생명공학대학 임상병리학과)
  • Received : 2009.12.30
  • Accepted : 2010.03.02
  • Published : 2010.06.30
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Abstract

Laminaria japonica polysaccharides (LP) were prepared from L. japonica through hot water extraction, ultrafiltration and gel chromatography. In this study, we investigated the immunomodulating activity of LP (0.25-1 mg/mL) on the mitogen/alloantigen reactive proliferation and killing activity of the Balb/c mouse splenocytes. The LP directly induced the proliferation of splenocytes that was stimulated with mitogen or alloantigen in a dose-dependent manner. The killing activity of cytotoxic T lymphocytes (CTLs) and lymphokine activated killer cells (LAKs) were enhanced significantly in the LP treated cells. Also, the treatment of splenocytes with LP increased production of interleukin-2 (IL-2). These results suggest that polysaccharides from L. japonica show a substantial immunomodulating activity in mouse immune cells.

Laminaria japonica의 polysaccharides(LP)가 마우스 비장 세포에서 현저한 면역 증강 활성을 보였다. Balb/c 마우스 비장 세포를 mitogen인 Con A, T cell growth factor인 IL-2 및 alloantigen인 anti-C57로 자극한 뒤, LP를 농도별(0.25, 0.5, 1 mg/mL)로 첨가하여 마우스 비장 세포의 증식 활성을 측정한 결과, 비장 세포증식능이 유의적으로 증가함을 확인하였다. 이러한 결과를 근거로 항원 특이적인 세포 독성 T 림프구(CTLs)의 활성과 IL-2에 의해 야기되는 비특이적인 림포카인 활성 살해 세포(LAKs)의 세포독성능을 측정하였다. 작동 세포와 표적 세포의 비율을 10:1로 혼합한 뒤, LP를 농도별로 첨가하여 세포 용해 시 발생되는 LDH를 측정한 결과, 동종 항원 반응 비장 세포의 살해 세포 작용이 유의적으로 증가하였으며, LAKs의 활성 또한 유의적으로 증가함을 확인하였다. 그리고 T 림프구의 활성에 직접적으로 관여하는 IL-2의 생산능을 측정한 결과, LP를 첨가한 경우 농도 의존적으로 IL-2의 생산량이 증가됨을 확인하였다. 이상의 결과로, 참다시마에서 획득한 LP가 마우스 비장 세포의 T 림프구 및 NK 세포의 증식에 영향을 미치는 면역 증강 활성을 가지고 있음을 입증하였다.

Keywords

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