Molecular detection of Aeromonas hydrophila isolated from albino catfish, Clarias sp. reared in an indoor commercial aquarium

  • Choresca, Casiano H. Jr. (Laboratory of Aquatic Animal Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Gomez, Dennis K. (Institute of Aquaculture, College of Fisheries and Ocean Sciences, University of the Philippines in the Visayas) ;
  • Han, Jee-Eun (Laboratory of Aquatic Animal Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Shin, Sang-Phil (Laboratory of Aquatic Animal Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Kim, Ji-Hyung (Laboratory of Aquatic Animal Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Jun, Jin-Woo (Laboratory of Aquatic Animal Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Park, Se-Chang (Laboratory of Aquatic Animal Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University)
  • Accepted : 2010.12.20
  • Published : 2010.12.30

Abstract

Moribund albino catfish, Clarias sp., displayed from an indoor private commercial aquarium were submitted in the laboratory for diagnostic examination. Dense culture of bacteria was recovered from the kidney and was characterized using Vitek System 2 and showed 98% probability to Aeromonas (A.) hydrophila. PCR result showed positive using A. hydrophila extracellular hemolysin gene ahh1 (130 bp) and aerolysin gene aerA (309 bp). The 16S rRNA gene was identical and exhibited 97% sequence similarity with the other known isolates of A. hydrophila available in the GenBank. In this paper, we reported the isolation and molecular detection of A. hydrophila from an albino catfish.

Keywords

References

  1. Agger WA, McCormick JD, Gurwith MJ. Clinical and microbiological features of Aeromonas hydrophila-associated diarrhea. J Clin Microbiol 1985, 21, 909-913.
  2. Angka SL, Lam TJ, Sin YM. Some virulence characteristics of Aeromonas hydrophila in walking catfish (Clarias gariepinus). Aquaculture 1995, 130, 103-112. https://doi.org/10.1016/0044-8486(94)00216-B
  3. Bauer AW, Kirby WM, Sherris JC, Truck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966, 45, 493-496.
  4. Chu WH, Lu CP. Multiplex PCR assay for the detection of pathogenic Aeromonas hydrophila. J Fish Dis, 2005, 28,437-441. https://doi.org/10.1111/j.1365-2761.2005.00628.x
  5. Choresca CH Jr., Kim JH, Gomez DK, Jang H, Joh SJ, Park SC. Isolation of Serratia fonticola from pirarucu Arapaima gigas. Korean J Vet Res 2008, 48, 89-92.
  6. Clinical and Laboratory Standards Institute (CLSI). Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria; Approved guideline. CLSI document M45-A. CLSI, Wayne, 2006.
  7. Kodama H, Nakanishi Y, Yamamoto F, Mikami T, Izawa H, Imagawa T, Hashimoto Y, Kudo N. Salmonella arizonae isolated from a pirarucu, Arapaima gigas Cuvier, with septicaemia. J Fish Dis 1987, 10, 509-512. https://doi.org/10.1111/j.1365-2761.1987.tb01103.x
  8. Lewbart GA. Bacteria and ornamental fish. Semin Avian Exot Pet Med 2001, 10, 48-56. https://doi.org/10.1053/saep.2001.19543
  9. Thune RL, Stanley LA, Cooper RK. Pathogenesis of gram-negative bacterial infections in warmwater fish. Annu Rev Fish Dis 1993, 3, 37-68. https://doi.org/10.1016/0959-8030(93)90028-A
  10. Viveiros ATM, So N, Komen J. Sperm cryopreservation of african catfish, Clarias gariepinus: cryoprotectants, freezing rates and sperm:egg dilution ratio. Theriogenology 2000, 54, 1395-1408. https://doi.org/10.1016/S0093-691X(00)00462-3
  11. Wang G, Clark CG, Liu C, Pucknell C, Munro CK, Kruk TMAC, Caldeira R, Woodward DL, Rodgers FG. Detection and characterization of the hemolysin genes in Aeromonas hydrophila and Aeromonas sobria by multiplex PCR. J Clin Microbiol 2003, 41, 1048-1054. https://doi.org/10.1128/JCM.41.3.1048-1054.2003
  12. Xia C, Ma ZH, Rahman MH, Wu ZG. PCR cloning and identification of the ${\beta}$-haemolysin gene of Aeromonas hydrophila from freshwater fishes in China. Aquaculture 2004, 229, 45-53. https://doi.org/10.1016/S0044-8486(03)00398-3