Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Monitoring Methylmercury in Abyssal Fish

심해성 어류 중 메틸수은 모니터링

  • Kim, Seong-Cheol (Imported Food Analysis Division, Center for Food and Drug analysis, Busan Regional Korea Food and Drug Administration) ;
  • Jang, Jin-Wook (Imported Food Analysis Division, Center for Food and Drug analysis, Busan Regional Korea Food and Drug Administration) ;
  • Kim, Hyun-Ah (Imported Food Analysis Division, Center for Food and Drug analysis, Busan Regional Korea Food and Drug Administration) ;
  • Lee, Sang-Ho (Imported Food Analysis Division, Center for Food and Drug analysis, Busan Regional Korea Food and Drug Administration) ;
  • Jung, Young-Ji (Imported Food Analysis Division, Center for Food and Drug analysis, Busan Regional Korea Food and Drug Administration) ;
  • Kim, Ji-Yeon (Imported Food Analysis Division, Center for Food and Drug analysis, Busan Regional Korea Food and Drug Administration) ;
  • Ahn, Jong-Hoon (Imported Food Analysis Division, Center for Food and Drug analysis, Busan Regional Korea Food and Drug Administration) ;
  • Park, Eun-Hye (Imported Food Analysis Division, Center for Food and Drug analysis, Busan Regional Korea Food and Drug Administration) ;
  • Ko, Yong-Seok (Cardiovascula and Neuropharmacological Drugs Division, Drug Evaluation Department, Korea Food and Drug Administration) ;
  • Kim, Dong-Sul (Food Investigation Science Team, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation) ;
  • Kim, Sang-Yub (Imported Food Analysis Division, Center for Food and Drug analysis, Busan Regional Korea Food and Drug Administration) ;
  • Jang, Young-Mi (Imported Food Analysis Division, Center for Food and Drug analysis, Busan Regional Korea Food and Drug Administration) ;
  • Kang, Chan-Soon (Imported Food Analysis Division, Center for Food and Drug analysis, Busan Regional Korea Food and Drug Administration)
  • 김성철 (부산지방식품의약품안전청 수입식품분석과) ;
  • 장진욱 (부산지방식품의약품안전청 수입식품분석과) ;
  • 김현아 (부산지방식품의약품안전청 수입식품분석과) ;
  • 이상호 (부산지방식품의약품안전청 수입식품분석과) ;
  • 정영지 (부산지방식품의약품안전청 수입식품분석과) ;
  • 김지연 (부산지방식품의약품안전청 수입식품분석과) ;
  • 안종훈 (부산지방식품의약품안전청 수입식품분석과) ;
  • 박은혜 (부산지방식품의약품안전청 수입식품분석과) ;
  • 고용석 (의약품안전국 순환계약품과) ;
  • 김동술 (식품의약품안전평가원 오염물질과) ;
  • 김상엽 (부산지방식품의약품안전청 수입식품분석과) ;
  • 장영미 (부산지방식품의약품안전청 수입식품분석과) ;
  • 강찬순 (부산지방식품의약품안전청 수입식품분석과)
  • Received : 2009.11.02
  • Accepted : 2010.04.12
  • Published : 2010.08.31
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Abstract

The aim of this study was to determine the methylmercury (MeHg) levels in abyssal fish species. The MeHg in the fishes was extracted with hydrochloric acid and toluene and then purified using an L-cysteine solution. The extract was analyzed with a gas chromatography-electron capture detector (GC-${\mu}ECD$) with a thermon Hg-capillary column. The detection limit and the recovery of the method were 0.002 and 84.2-98.5% (mean, 93.4%), respectively. The MeHg content in 492 abyssal fishes ranged from 0.037 to 2.009 mg/kg. The levels of MeHg [range, mg/kg (mean)] were significantly dependent on fish species and presented as the following; 0.157-2.009 (0.546) in Scalloped hammerhead shark, 0.211-0.878 (0.501) in Blue shark, 0.121-0.993 (0.482) in Spiny dogfish, 0.243-0.658 (0.397) in Salmon shark, 0.074-1.958 (0.353) in Blacktip shark, 0.038-0.807 (0.302) in Southern hake, 0.099-0.511 (0.300) in Scorpion fish, and 0.037-0.133 (0.067) in Ling. The monitoring results showed that the estimated weekly intake of MeHg from sharks, Southern hake, and Ling were lower than the provisional tolerable weekly intake recommended by the Joint FAO/WHO expert committee on food additives.

심해성 어류 등에 대한 메틸수은 기준규격 설정에 따른 사후 관리 차원의 모니터링을 실시하였다. 본 연구에 사용된 어종은 귀상어, 흑기흉상어, 곱상어, 청새리상어, 악상어, 적어, 은민대 및 체장메기 등 총 492건을 수거하여 메틸수은 함량을 조사하였다. 메틸수은 분석을 위한 분석법 타당성을 검토한 결과 GC-${\mu}ECD$의 검출한계와 정량한계는 0.002 mg/kg과 0.005 mg/kg으로 나타났으며 메틸수은의 검량선은 상관계수가 0.9992로 우수한 직선성을 보였고, 회수율은 84.2-98.5%(평균 93.4%)로 나타났다. 심해성 어류의 메틸수은 함량은 각 어종 별로 유의적인 차이가 있었으며, 메틸수은 함량[최소값-최대값(평균$\pm$표준편차), 단위: mg/kg]은 귀상어 0.157-2.009($\0.546{pm}0.311$), 청새리상어 0.211-0.878($0.501{\pm}0.154$) 및 곱상어 0.121-0.993($0.482{\pm}0.189$) 등에서 높게 나타났으며, 악상어 0.243-0.658($0.397{\pm}0.090$), 흑기흉상어 0.074-1.958($0.353{\pm}0.418$), 은민대구 0.038-0.807($0.302{\pm}0.201$) 및 적어 0.099-0.511($0.300{\pm}0.094$) 등에서는 낮은 것으로 나타났고, 체장메기의 경우 0.037-0.133($0.067{\pm}0.016$)으로 나타나 조사된 어종 중유의적으로 가장 낮은 함량을 나타내었다. 본 연구에서 조사된 심해성 어류 중 메틸수은 함량의 노출량 평가를 위해 JECFA의 PTWI와 비교 검토한 결과 상어류 0.7%, 은민대구 1.1% 및 체장 메기 0.3% 수준으로 나타났다. 또한 극단적인 노출량 평가를 위해서 어종별 메틸수은 최대 함량을 이용하여 산출한 결과도 상어류 2.9%, 은민대구 2.9% 및 체장메기 0.6% 수준으로 나타나 심해성 어류의 섭취로 인한 메틸수은 노출량은 안전한 수준인 것으로 판단된다.

Keywords

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