Plant Biotechnology Reports

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Sll0396 regulates transcription of the phycocyanin genes in Synechocystis sp. PCC 6803

  • Oh, In-Hye (Department of Biology and Medicinal Science, PaiChai University) ;
  • Kim, Ho-San (Mass Spectrometer Research Center, Korea Basic Science Institute) ;
  • Chung, Young-Ho (Mass Spectrometer Research Center, Korea Basic Science Institute) ;
  • Kim, Young-Hye (Mass Spectrometer Research Center, Korea Basic Science Institute) ;
  • Park, Young-Mok (Mass Spectrometer Research Center, Korea Basic Science Institute)
  • Received : 2009.12.03
  • Accepted : 2010.02.17
  • Published : 2010.09.30
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Abstract

An olive-green mutant was generated in Synechocystis sp. strain PCC 6803 by inactivation of the sll0396 gene. Whole-cell absorption spectra of the mutant revealed the missing of phycocyanin peak. An investigation of the low-temperature fluorescence emission spectra revealed that the $sll0396{\Omega}$ mutant has a reduced amount of phycocyanin. Western blot analysis showed that the mutant contained less phycocyanin ${\beta}$- and ${\alpha}$-subunits and lacked the 30- and 32-kDa linker polypeptides, and northern blot analysis revealed that the transcription of the 1.4-kb cpcBA gene encoding the phycocyanin ${\beta}$- and ${\alpha}$-subunits was lower in the mutant. The Sll0396 protein has a DNA-binding motif and shares homology with known response regulators. Our results indicate that Sll0396 plays a regulatory role in the transcription of the phycocyanin genes during phycobilisome synthesis.

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References

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