The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anti-oxidative effects of fractionated Pulsatilla koreana $N_{AKAI}$ extracts

백두옹 분획층의 항산화 효과

  • Cho, Hyun-Jin (Dept. of Prescriptionology, College of Oriental Medicine, Dongguk University.) ;
  • Yun, Hyun-Jeong (Dept. of Prescriptionology, College of Oriental Medicine, Dongguk University.) ;
  • Yi, Hyo-Seung (Dept. of Prescriptionology, College of Oriental Medicine, Dongguk University.) ;
  • Park, Sun-Dong (Dept. of Prescriptionology, College of Oriental Medicine, Dongguk University.)
  • 조현진 (동국대학교 한의과대학 방제학교실) ;
  • 윤현정 (동국대학교 한의과대학 방제학교실) ;
  • 이효승 (동국대학교 한의과대학 방제학교실) ;
  • 박선동 (동국대학교 한의과대학 방제학교실)
  • Received : 2010.05.01
  • Accepted : 2010.06.22
  • Published : 2010.06.30
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Abstract

Objectives : This study was designed to investigate the effects of anti-oxidation of fractionated Pulsatilla koreana $N_{AKAI}$ (PK) extracts. And we examined to determine that a certain fractionated extract has the best anti-oxidative effects between the fractionated PK extracts. Methods : Anti-oxidative effects of fractionated PK extracts was measured by scavenging activities of DPPH, superoxide, nitric oxide (NO) and peroxynitrite radicals. And also scavenging activities of anti-oxidation in lipopolysaccharide (LPS)-treated RAW 264.7 cell was measured. After these examination, we determined a fraction that has best anti-oxidative effects. Results : Fractionated PK extracts inhibited radicals effectively. Also in RAW 264.7 cell, intracellular oxidation has inhibited by PK extracts. In these tests, ethyl acetate (EA) fraction has the best anti-oxidative effects among PK extracts. Conclusions : This results demonstrate that PK extracts exhibit anti-oxidative effects. And EA fraction has the best inhibition effects among the six fractions of PK.

Keywords

References

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