Stimulation of the Immune Response by Herbal Formulas for Wind-Cold and Heat Pain Symptom

대표적인 풍한열(風寒熱)에 의한 통증 치료 처방의 면역 활성화 비교 연구

  • Jung, Da-Young (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Ha, Hye-Kyung (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Ho-Young (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Jin-Ah (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Jun-Kyoung (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Huang, Dae-Sun (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
  • 정다영 (한국한의학연구원 한약EBM연구센터) ;
  • 하혜경 (한국한의학연구원 한약EBM연구센터) ;
  • 이호영 (한국한의학연구원 한약EBM연구센터) ;
  • 이진아 (한국한의학연구원 한약EBM연구센터) ;
  • 이준경 (한국한의학연구원 한약EBM연구센터) ;
  • 황대선 (한국한의학연구원 한약EBM연구센터) ;
  • 신현규 (한국한의학연구원 한약EBM연구센터)
  • Received : 2010.06.14
  • Accepted : 2010.08.02
  • Published : 2010.08.25

Abstract

Three herbal formulas (Bangpungtongsung-san, Ohyaksungi-san, and Ojeok-san) for wind-cold and heat pain symptom were applied to investigate the immunological activities on antigen (Ag)-specific or Ag-non-specific immune responses in murine macrophage cell line (RAW 264.7) and ovalbumin (OVA)-immunized mice. This study was carried out in nitric oxide (NO) synthesis in RAW 264.7 cells and cellular proliferation in mouse splenocytes according to three herbal formulas. C57BL/6 mice were immunized intraperitonially with OVA/aluminium ($100\;{\mu}g/200\;{\mu}g$/mouse) on day 1, 8, and 15. Three herbal formulas were administrated to mice orally for 3 weeks from day 1. On day 22, OVA-, lipopolysaccharide (LPS)-, and concanavalin A (Con A)-stimulated splenocyte proliferation and antibodies (OVA-specific antibodies of the IgG, lgG1, and total IgM classes) in plasma were measured. Ohyaksungi-san increased NO synthesis in RAW 264.7 cells. Ojeok-san and Ohyaksungi-san significantly enhanced cellular proliferation by LPS and Con A in splenocytes from OVA-immunized mice (p<0.001). Three herbal formulas for wind-cold and heat pain symptom also significantly enhanced plasma OVA-specific IgG, IgG1, and total IgM levels compared with the OVA/Alum group. These results suggested that three herbal formulas for wind-cold and heat pain symptom could be used as stimulator of immune response.

Keywords

Acknowledgement

Supported by : 한국한의학연구원

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