YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Anti-oxidant and Anti-aging Activities of Essential Oils of Pinus densiflora Needles and Twigs

소나무 잎과 가지의 정유와 그 성분들의 항산화 및 항노화 작용

  • Kim, Youn-Joung (Division of Pathophysiology College of Pharmacy Chung-Ang University) ;
  • Cho, Bong-Jae (Division of Pathophysiology College of Pharmacy Chung-Ang University) ;
  • Ko, Myung-Soo (Division of Pathophysiology College of Pharmacy Chung-Ang University) ;
  • Jung, Jae-Min (Division of Pathophysiology College of Pharmacy Chung-Ang University) ;
  • Kim, Hee-Rae (Division of Pathophysiology College of Pharmacy Chung-Ang University) ;
  • Song, Ho-Sun (Division of Pathophysiology College of Pharmacy Chung-Ang University) ;
  • Lee, Ji-Yun (Division of Pathophysiology College of Pharmacy Chung-Ang University) ;
  • Sim, Sang-Soo (Division of Pathophysiology College of Pharmacy Chung-Ang University) ;
  • Kim, Chang-Jong (Division of Pathophysiology College of Pharmacy Chung-Ang University)
  • 김연정 (중앙대학교 약학대학 병태생리학교실) ;
  • 조봉재 (중앙대학교 약학대학 병태생리학교실) ;
  • 고명수 (중앙대학교 약학대학 병태생리학교실) ;
  • 정재민 (중앙대학교 약학대학 병태생리학교실) ;
  • 김희래 (중앙대학교 약학대학 병태생리학교실) ;
  • 송호선 (중앙대학교 약학대학 병태생리학교실) ;
  • 이지윤 (중앙대학교 약학대학 병태생리학교실) ;
  • 심상수 (중앙대학교 약학대학 병태생리학교실) ;
  • 김창종 (중앙대학교 약학대학 병태생리학교실)
  • Received : 2010.01.20
  • Accepted : 2010.06.10
  • Published : 2010.08.31
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Abstract

To investigate effects of pine essential oil isolated by steam distillation from Pinus densiflora needles (PN) and twigs (PT) on anti-oxidant and anti-aging activities, the experiments were carried out to determine anti-oxidant and antiaging activities on DPPH radical scavenging activity, NBT/xanthine oxidase-superoxide scavenging activity, silica-induced intracellular $H_2O_2$ and hydroxyl radical generation in RAW264.7 cells, hyaluronidase and elastase activities in vitro. Essential oil of PN and PT were contained 0.225 and 0.176 (w/v) %, respectively. PN was contained with 30 kinds of essential oil and its major constituent is $\alpha$-pinene (21.5%). Further PT was contained with 40 kinds of essential oil and its major constituent is $\beta$-pinene (22.4%) in GC/MS assay. Other essential oils of PN were $\beta$-pinene, camphene, myrcene, limonene, terpinolene, $\alpha$-terpineol, bornyl acetate, $\alpha$-copaene, caryophellene and humulene and PT were $\alpha$-pinene, camphene, phellandrene, limonene, terpinolene, $\alpha$-terpineol, bornyl acetate, $\alpha$-copaene, caryophellene and humulene. The essential oil of PT have more active than that of PN in anti-oxidant activity which has significant DPPH radical and superoxide scavenging activity, and significant inhibitory activities on silica-induced intracellular $H_2O_2$ and hydroxyl radical generation, as well as, significantly inhibited elastase and hyaluronidase activities. Further, phellandrene, myrcene and $\alpha$-pinene have DPPH radical and superoxide scavenging activities, $\beta$-pinene, terpinolene, myrcene and phellandrene inhibited silica-induced intracellular $H_2O_2$ and hydroxyl radical generations. And also phellandrene and $\beta$-pinene inhibited hyaluronidase and elastase activities. In conclusion, the essential oils isolated from PN and PT have anti-oxidant and anti-aging activities.

Keywords

References

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