YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Antimicrobial Activities of Eight Compounds Purified from the Roots of Polygala tenuifolia Willdenow and the Aerial Bulbils of Dioscorea batatas Decene

원지와 영여자로부터 분리한 8종 화합물의 항균효과

  • Chin, Hwi-Seung (R&D Center, Jung San Bio Technology) ;
  • Son, Rak-Ho (R&D Center, Jung San Bio Technology) ;
  • Lee, Yong-Hwa (R&D Center, Jung San Bio Technology) ;
  • Ham, Ah-Rom (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Mar, Woong-Chon (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Kim, Won-Ki (Department of Neuroscience, College of Medicine, Korea University) ;
  • Nam, Kung-Woo (R&D Center, Jung San Bio Technology)
  • Received : 2009.09.09
  • Accepted : 2009.12.08
  • Published : 2010.04.30
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Abstract

The chemical structures of eight compounds purified from two plants (Polygala tenuifolia Willdenow and Dioscorea batatas Decene) were determined and their anti-microbial activity against three microbial strains (Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans) was tested. The three micro organisms were cultured in 96-well plates or Petri dishes without (control) or with the eight compounds added at concentrations of 100 to 0.01 ${\mu}M$ (wt/vol). The growth of the microorganisms in the medium was examined after a 24-h incubation. The inhibitory effect of each compound on the growth of the microorganisms was calculated from the optical density measured at 595 nm, turbidity, and size of the inhibition zone around the treated paper disc. The minimum inhiitory concentration (MIC) of compounds 4 to 7 against S. aureus was 0.08, 0.05, 1.3 and 0.02 ${\mu}M$, respectively, and 0.09, 0.1, 0.2 and 100 ${\mu}M$ against C. albicans. The $IC_{50}$ (50% inhibition) values of compounds 5 and 6 were 3.1 and 6.4 ${\mu}M$ against S. aureus, respectively, and 10 and 2.4 ${\mu}M$ against C. albicans. Therefore, compounds 4 to 6 were the most potent anti-microbial agents among the eight compounds tested.

Keywords

References

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