대한한방내과학회지 (The Journal of Internal Korean Medicine)

대한한방내과학회 (The Society of Internal Korean Medicine)
권호 표지

$MPP^+$로 유도된 SH-SY5Y신경세포 사멸에 대한 고분자성분제거 봉독약침액의 신경보호 효과 연구

Neuroprotective Effects of Bee Venom, which Removes High Molecular Elements against $MPP^+$-induced Human Neuroblastoma SH-SY5Y Cell Death

  • 배광록 (경희대학교 동서의학대학원 동서의학과) ;
  • 두아름 (경희대학교 한의과대학 경혈학교실, 침구경락과학연구센터) ;
  • 김승남 (경희대학교 한의과대학 경혈학교실, 침구경락과학연구센터) ;
  • 박지연 (경희대학교 한의과대학 경혈학교실, 침구경락과학연구센터) ;
  • 박히준 (경희대학교 한의과대학 경혈학교실, 침구경락과학연구센터) ;
  • 이혜정 (경희대학교 한의과대학 경혈학교실, 침구경락과학연구센터) ;
  • 권기록 (상지대학교 한의과대학)
  • Bae, Kwang-Rok (Dept. of East-West Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University) ;
  • Doo, Ah-Reum (Dept. of Meridian & Acupuncture, College of Korean Medicine, Kyung-Hee University, Acupuncture and Meridian Science Research Center) ;
  • Kim, Seung-Nam (Dept. of Meridian & Acupuncture, College of Korean Medicine, Kyung-Hee University, Acupuncture and Meridian Science Research Center) ;
  • Park, Ji-Yeon (Dept. of Meridian & Acupuncture, College of Korean Medicine, Kyung-Hee University, Acupuncture and Meridian Science Research Center) ;
  • Park, Hi-Joon (Dept. of Meridian & Acupuncture, College of Korean Medicine, Kyung-Hee University, Acupuncture and Meridian Science Research Center) ;
  • Lee, Hye-Jung (Dept. of Meridian & Acupuncture, College of Korean Medicine, Kyung-Hee University, Acupuncture and Meridian Science Research Center) ;
  • Kwon, Ki-Rok (Dept. of Acupuncture & Moxibustion, College of Korean Medicine, Sang-Ji University)
  • 발행 : 2010.06.30
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초록

Objectives : The neuroprotective effects of bee venom (BV) have been demonstrated in many studies, but bee venom has many side effects. So we used sweet bee venom (SBV), which has high molecular elements removed to reduce the side effects. I examined the neuroprotective effect of sweet bee venom in 1-methyl-4-phenylpyridine ($MPP^+$)-induced human neuroblastoma SH-SY5Y cells. Methods : To observe the possible toxicity of SBV itself, SH-SY5Y cells were treated with SBV in various concentrations for 3 h and $MPP^+$ in concentrations (1 and 5mM) for 24h. To investigate the protective effect of SBV against $MPP^+$ toxicity, SH-SY5Y cells were pretreated with vehicle or nontoxic concentrations of SBV for 3h and the cells were not washed, followed by incubation with respective concentrations of SBV and 1 mM $MPP^+$ for 24h. To investigate the protective effect of SBV against $MPP^+$ toxicity, SH-SY5Y cells were pretreated with vehicle or nontoxic concentrations of SBV for 3h and the cells were not washed, followed by incubation with respective of SBV(0.5%), 1 mM $MPP^+$, 5uM AKT inhibitor(LY984002) and 10uM ERK inhibitor(PD98059) for 24 h. The protective effect was measured by cell viability assay. To investigate the degree of apoptosis, caspase-3 enzyme activity was measured in control, $MPP^+$, SBV+$MPP^+$. Results : SBV (0.5%) pretreatment protected the SH-SY5Y cells against $MPP^+$-induced apoptotic cell death. The cell viability was higher in the SH-SY5Y cells that were pretreated with vehicle or nontoxic concentrations of SBV than those not pretreated. The caspase-3 activity was lower in the pretreated groups than these not pretreated. ERK and AKT enzymes have a role in the neuroprotective effects of the sweet bee venom. Conclusions : The results demonstrate that SBV has a protective effect on dopaminergic neurons against $MPP^+$ toxicity. This data suggest that SBV could be a potential therapeutic tool for neurodegenerative diseases such as Parkinson's disease(PD).

키워드

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