Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Characterization of Triterpenoids, Flavonoids and Phenolic Acids in Eclipta prostrata by High-performance Liquid Chromatography/diode-array Detector/electrospray Ionization with Multi-stage Tandem Mass Spectroscopy

  • Lee, Ki-Yong (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University) ;
  • Ha, Na-Ry (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University) ;
  • Kim, Tae-Bum (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University) ;
  • Kim, Young-Choong (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University) ;
  • Sung, Sang-Hyun (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University)
  • Received : 2010.07.26
  • Accepted : 2010.09.04
  • Published : 2010.09.30
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Abstract

High-performance liquid chromatographic method with diode-array detector and electrospray ionization with multi-stage tandem mass spectroscopy (HPLC/DAD/ESI-$MS^n$) was used to identify the major constituents in a methanolic extract of Eclipta prostrata. The chromatographic separation was performed on a C18 column. Acetonitrile-water was used as a mobile phase. HPLC/DAD/ESI-$MS^n$ allowed the characterization of constituents of E. prostrata, mainly triterpenoids (eclalbasaponin I, II, III, IV, VI), flavonoids (luteolin 7-O-glucoside, demethylwedelolactone, wedelolactone, luteolin, demetylwedelolactone sulfate, luteolin sulfate, apigenin sulfate) and phenolic acids (5-O-caffeoylquinic acid, 3, 4-O-dicaffeoylquinic acid, 3, 5-O-dicaffeoylquinic acid, 4, 5-Odicaffeoylquinic acid).

Keywords

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