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The Korean Society of Plant Pathology (한국식물병리학회)
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Genetic Diversity of Fusarium proliferatum Populations from Maize, Onion, Rice and Sugarcane in Iran Based on Vegetative Compatibility Grouping

  • Received : 2010.05.05
  • Accepted : 2010.05.26
  • Published : 2010.09.01
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Abstract

Fusarium proliferatum is the causal agent of stalk and root rot disease of maize, foot rot disease of rice, basal and root rot disease of onion and knife cut disease of sugarcane in Iran. In recent years, incidence and severity of these diseases have been increased in Iran. Fifty seven F. proliferatum single-spore isolates collected from diseased maize, rice, onion and sugarcane plants at different areas were used to study genetic diversity by determination of vegetative compatibility groups (VCGs). Chlorate-resistant nitrate non-utilizing (nit) mutants were recovered from selected isolates of F. proliferatum and used in complementation tests. All isolates in which both nit1 and NitM (or nit3) mutants were recovered, demonstrated self-compatibility. Vegetative compatibility tests by pairing nit mutants identified 30 VCGs among 57 isolates. Twenty-three isolates belonged to singlemember VCGs and the remaining 34 isolates, belonged to other seven multimember VCGs. Segregation of F. proliferatum isolates obtained from various area and host plants into different VCGs in Iran is reported for the first time. In this study, none of isolates obtained from rice complemented with any other isolates from onion and sugarcane and, non complementation occurred between onion and sugarcane isolates. Also, only one complementation occurred between one isolate of maize and one isolate of sugarcane and rice. Thus, a correlation between VCGs grouping and host preferences was founded. It is concluded that natural populations of F. proliferatum in Iran are probably genetically divergent and include isolates representing a potential risk for disease development.

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References

  1. Abbaszadeh, M., Nikkhah, M. J., Padasht Dehkayi, F., Mousanejad, S. 2007. Sexual fertility and mating types of Gibberella fujikuroi species complex, the cause of Bakanae disease and foot rot in Guilan province, Iran. Iran. J. Agri. Sci. 38:685-692.
  2. Abd-Elsalam, K. A., Schnieder, F., Asran-Amal, A., Khalil, M. S. and Vereet, J. A. 2003. Intra-species genomic groups in Fusarium semitectum and their correlation with origin and cultural characteristics. Z Pflanzenkr Pflanzenschutz 110:409-418.
  3. Alian, A., Javan-Nikkhah, M., Aminian, H. and Khosravi, V. 2005. Study on Genetic Diversity of Gibberella intermedia, The Common Causal Agent of Rice Bakanae Disease in Mazandaran Province, based on Vegetative Compatibility Groups. Iran. J. Agri. Sci. 38:685-692.
  4. Alizadeh, A. 2010. Study on genetic diversity of Gibberella intermedia isolates recovered from maize, rice and sugarcane using AFLPs molecular marker and vegetative compatibility groups (VCGs). M.Sc. thesis, University of Tehran, Iran.
  5. Alizadeh, A., Javan-Nikkhah, M., Fotouhifar, Kh., Mohammadian, E., Rabiee Motlagh, E. and Ghazanfari, K. 2009. Fertility status and PCR based identification of mating type alleles in Gibberella intermedia populations from maize, rice, onion and sugarcane in Iran. Asian Mycological Congress (AMC 2009), Taiwan.
  6. Bargen, V. S., Martinez, O., Schadock, I., Eisold, A. M., Gossmann, M. and Buttner, C. 2009. Genetic Variability of Phytopathogenic Fusarium proliferatum Associated with Crown Rot in Asparagus officinalis. J. Phytopathol. 157: 446-456. https://doi.org/10.1111/j.1439-0434.2008.01525.x
  7. Bujari, J., Ershad, D., 1993. An investigation on corn seed mycoflora. Iran. J. Plant Pathol. 29:13-17.
  8. Campbell, C. L., Leslie, J. F. and Farrokhi-Nejad, R. 1992. Genetic diversity of Fusarium moniliforme in seed from two maize cultivars. Phytopathology 82:1082.
  9. Correll, J. C., Klittich, C. J. R. and Leslie, J. F. 1987. Nitrate nonutilizing mutants of Fusarium oxysporum and their use in vegetative compatibility test. Phytopathology 77:1640-1646. https://doi.org/10.1094/Phyto-77-1640
  10. Chulze, S. N., Ramirez, M. L., Torres, A. and Leslie, J. F. 2000. Genetic variation in Fusarium section Liseola from no-till maize in Argentina. Appl. Environ. Microbiol. 66:5312-5315. https://doi.org/10.1128/AEM.66.12.5312-5315.2000
  11. Cumagun, C. J. R., Bowden, R. L. and Miedaner, T. 2002. Segregation of aggressiveness in a crossing population of Fusarium graminearum. J. Appl. Genet. 43:39-44.
  12. Danielsen, S., Meyer, U. M. and Funck Jensen, D. 1998. Genetic characteristics of Fusarium verticillioides isolates from maize in Costa Rica. Plant Pathol. 44:615-622.
  13. Desjardins, A. E. 2003. Giberella from A(venaceae to Z(eae). Annu. Rev. Phytopathol. 41:177-198. https://doi.org/10.1146/annurev.phyto.41.011703.115501
  14. Farrokhi-Nejad, R. and Leslie, J. F. 1990. Vegetative compatibility group diversity with populations of Fusarium moniliforme isolates from corn seed. Phytopathology 80:1043.
  15. Galvan, G. A., Koning-Boucoiran, C. F. S., Koopman, W. J. M., Burger-Meijer, K., Gonzalez, P. H., Waalwijk, C., Kik, C. and Scholten, O. E. 2008. Genetic variation among Fusarium isolates from onion and resistance to Fusarium basal rot in related Allium species. Eur. J. Plant Pathol. 121:499-512. https://doi.org/10.1007/s10658-008-9270-9
  16. Ghiasian, S. A., Kord-Bacheh, P., Rezayat, S. M., Maghsood, A. H. and Taherkhani, H. 2004. Mycoflora of Iranian maize harvested in the main production areas in 2000. Mycopathologia 158:113-121. https://doi.org/10.1023/B:MYCO.0000038425.95049.03
  17. Klittich, C. J. R. and Leslie, J. F. 1988a. Nitrate reduction mutants of Fusarium moniliforme (Gibberella fujikuroi). Genetics 118: 417-423.
  18. Klittich, C. J. R. and Leslie, J. F. 1988b. Multiwell plates for complementation tests of Fusarium. Fungal Gen. Newsletter 35:21-22.
  19. Leslie, J. F. 1991. Mating populations in Gibberella fujikuroi (Fusarium section Liseola). Phytopathology 81:1058-1060.
  20. Leslie, J. F. 1993. Fungal vegetative compatibility. Annu. Rev. Phytopathol. 31:127-151. https://doi.org/10.1146/annurev.py.31.090193.001015
  21. Leslie, J. F. 1995. Gibberella fujikuroi: available populations and variable traits. Can. J. Bot. 73:282-291. https://doi.org/10.1139/b95-258
  22. Leslie, J. F. and Klein, K. K. 1996. Female fertility and matingtype effects on effective population size in filamentous fungi. Genetics 144:557-576.
  23. Leslie, J. F. and Summerell, B. A. 2006. The Fusarium Laboratory Manual. Blackwell Publishing Professional, Ames, USA. 388 pp.
  24. McDonald, B. A. 2004. Population genetics of plant pathogens. The Plant Health Instructor DOI, 10.1094/PHI-A-2004-0524-01.
  25. McDonald, B. A. and McDermott, J. M. 1993. Population genetics of plant pathogenic fungi. Bioscience 43:311-319. https://doi.org/10.2307/1312063
  26. Milgroom, M. G. 1996. Recombination and the multilocus structure of fungal populations. Annu. Rev. Phytopathol. 34:457-477. https://doi.org/10.1146/annurev.phyto.34.1.457
  27. Mohammadian, E. 2008. Study on genetic diversity of Gibberella moniliformis and G. intermedia from corn and rice and identification of frequency of MAT locus alleles based on PCR. M.Sc. thesis, University of Tehran, Karaj, Iran.
  28. Nelson, P. E., Toussoun, T. A. and Marasas, W. F. O. 1983. Fusarium species: an illustrated manual for identification. The Pennsylvania State University Press, University Park, USA. 203 pp.
  29. Nitzan, N., Hazanovsky, M., Tal, M. and Tsror, L. 2002. Vegetative Compatibility Groups in Colletotrichum coccodes, the Causal Agent of Black Dot on Potato. Phytopathology 92: 827-832 https://doi.org/10.1094/PHYTO.2002.92.8.827
  30. Puhalla, J. E. 1985. Classification of strains of Fusarium oxysporum on the basis of vegetative compatibility. Can. J. Bot. 63:179-183 https://doi.org/10.1139/b85-020
  31. Rabiee motlagh, E. 2009. Study on phytopatpgenic fungi of root and bulb of onion in Razavi and north Khorasan provinces. M.Sc. thesis, ferdowsi University of Mashhad, Mashhad, Iran.
  32. Rahjoo, V., Zad, J., Javan-Nikkhah, M., Mirzadi Gohari, A., Okhovvat, S. M., Bihamta, M. R., Razzaghian, J. and Klemsdal, S. S. 2008. Molecular and morphological identification of fusarium isolated from maize ears in Iran. J. Plant Pathol. 90:463-468.
  33. Solomonson, L. P. and Vennesland, B. 1972. Nitrate reductase and chlorate toxicity in Chlorella vulgaris Beijerinck. Plant physiol. 50:421-424. https://doi.org/10.1104/pp.50.4.421
  34. Taherkhani, K. 1995. Study of sugarcane fusariosis diseases in khouzestan province. M.Sc. thesis, University of Tarbiat Modarres, Tehran, Iran.

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