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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Smad4 mediates malignant behaviors of human ovarian carcinoma cell through the effect on expressions of E-cadherin, plasminogen activator inhibitor-1 and VEGF

  • Chen, Chen (Key Laboratory for Proteomics of Liaoning Province, Dalian Medical University) ;
  • Sun, Ming-Zhong (Key Laboratory for Proteomics of Liaoning Province, Dalian Medical University) ;
  • Liu, Shuqing (Key Laboratory for Proteomics of Liaoning Province, Dalian Medical University) ;
  • Yeh, Dongmei (Key Laboratory for Proteomics of Liaoning Province, Dalian Medical University) ;
  • Yu, Lijun (Key Laboratory for Proteomics of Liaoning Province, Dalian Medical University) ;
  • Song, Yang (Key Laboratory for Proteomics of Liaoning Province, Dalian Medical University) ;
  • Gong, Linlin (Key Laboratory for Proteomics of Liaoning Province, Dalian Medical University) ;
  • Hao, Lihong (Key Laboratory for Proteomics of Liaoning Province, Dalian Medical University) ;
  • Hu, Jun (Key Laboratory for Proteomics of Liaoning Province, Dalian Medical University) ;
  • Shao, Shujuan (Key Laboratory for Proteomics of Liaoning Province, Dalian Medical University)
  • Received : 2010.04.08
  • Accepted : 2010.07.20
  • Published : 2010.08.31
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Abstract

Smad4 is involved in cancer progression and metastasis. Using a pair of human syngeneic epithelial ovarian cancer cells with low (HO-8910) and high (HO-8910PM) metastatic abilities, we aimed to reveal the role of Smad4 in ovarian cancer metastasis in vitro. Smad4 was down-regulated in HO-8910PM cell line relative to HO-8910 by implicating Smad4 was probably a potential tumor suppressor gene for ovarian cancer. Re-expression of Smad4 decreased the migration ability and inhibited the invasion capacity of HO-8910PM, while promoted the cell adhesion capacity for HO-8910PM. The stable expression of Smad4 increased the expression of E-cadherin, reduced the expression of plasminogen activator inhibitor-1 (PAI-1) and slightly down-regulated the expression of VEGF. Smad4 suppresses human ovarian cancer cell metastasis potential through its effect on the expressions of PAI-1, E-cadherin and VEGF. Results from current work implicate Smad4 might suppress the invasion and metastasis of human ovarian tumor cells through a TGF-$\beta$/Smad-mediated pathway.

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References

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